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. 1998 Mar;148(3):1031–1041. doi: 10.1093/genetics/148.3.1031

The Neurospora rca-1 gene complements an Aspergillus flbD sporulation mutant but has no identifiable role in Neurospora sporulation.

W C Shen 1, J Wieser 1, T H Adams 1, D J Ebbole 1
PMCID: PMC1460021  PMID: 9539422

Abstract

The Aspergillus nidulans flbD gene encodes a protein with a Myb-like DNA-binding domain that is proposed to act in concert with other developmental regulators to control initiation of conidiophore development. We have identified a Neurospora crassa gene called rca-1 (regulator of conidiation in Aspergillus) based on its sequence similarity to flbD. We found that N. crassa rca-1 can complement the conidiation defect of an A. nidulans flbD mutant and that induced expression of rca-1 caused conidiation in submerged A. nidulans cultures just as was previously observed for overexpression of flbD. Thus, the N. crassa gene appears to be a functional homologue of A. nidulans flbD and this is the first demonstration of functional complementation of an A. nidulans sporulation defect using a gene from an evolutionarily distant fungus. However, deletion of the rca-1 gene in N. crassa had no major effect on growth rate, macroconidiation, microconidiation, or ascospore formation. The only phenotype displayed by the rca-1 mutant was straight or counterclockwise hyphal growth rather than the clockwise spiral growth observed for wild type. Thus, if rca-1 is involved in N. crassa development, its role is subtle or redundant.

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Selected References

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  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Käfer E. Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet. 1977;19:33–131. doi: 10.1016/s0065-2660(08)60245-x. [DOI] [PubMed] [Google Scholar]
  4. Lee B. N., Adams T. H. FluG and flbA function interdependently to initiate conidiophore development in Aspergillus nidulans through brlA beta activation. EMBO J. 1996 Jan 15;15(2):299–309. [PMC free article] [PubMed] [Google Scholar]
  5. Lee B. N., Adams T. H. Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development. Mol Microbiol. 1994 Oct;14(2):323–334. doi: 10.1111/j.1365-2958.1994.tb01293.x. [DOI] [PubMed] [Google Scholar]
  6. Lüscher B., Eisenman R. N. New light on Myc and Myb. Part II. Myb. Genes Dev. 1990 Dec;4(12B):2235–2241. doi: 10.1101/gad.4.12b.2235. [DOI] [PubMed] [Google Scholar]
  7. Mirabito P. M., Adams T. H., Timberlake W. E. Interactions of three sequentially expressed genes control temporal and spatial specificity in Aspergillus development. Cell. 1989 Jun 2;57(5):859–868. doi: 10.1016/0092-8674(89)90800-3. [DOI] [PubMed] [Google Scholar]
  8. Myrset A. H., Bostad A., Jamin N., Lirsac P. N., Toma F., Gabrielsen O. S. DNA and redox state induced conformational changes in the DNA-binding domain of the Myb oncoprotein. EMBO J. 1993 Dec;12(12):4625–4633. doi: 10.1002/j.1460-2075.1993.tb06151.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ogata K., Hojo H., Aimoto S., Nakai T., Nakamura H., Sarai A., Ishii S., Nishimura Y. Solution structure of a DNA-binding unit of Myb: a helix-turn-helix-related motif with conserved tryptophans forming a hydrophobic core. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6428–6432. doi: 10.1073/pnas.89.14.6428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ogata K., Morikawa S., Nakamura H., Sekikawa A., Inoue T., Kanai H., Sarai A., Ishii S., Nishimura Y. Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices. Cell. 1994 Nov 18;79(4):639–648. doi: 10.1016/0092-8674(94)90549-5. [DOI] [PubMed] [Google Scholar]
  11. Oppenheimer D. G., Herman P. L., Sivakumaran S., Esch J., Marks M. D. A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell. 1991 Nov 1;67(3):483–493. doi: 10.1016/0092-8674(91)90523-2. [DOI] [PubMed] [Google Scholar]
  12. PONTECORVO G., ROPER J. A., HEMMONS L. M., MACDONALD K. D., BUFTON A. W. J. The genetics of Aspergillus nidulans. Adv Genet. 1953;5:141–238. doi: 10.1016/s0065-2660(08)60408-3. [DOI] [PubMed] [Google Scholar]
  13. Paz-Ares J., Ghosal D., Wienand U., Peterson P. A., Saedler H. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J. 1987 Dec 1;6(12):3553–3558. doi: 10.1002/j.1460-2075.1987.tb02684.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perkins D. D., Radford A., Newmeyer D., Björkman M. Chromosomal loci of Neurospora crassa. Microbiol Rev. 1982 Dec;46(4):426–570. doi: 10.1128/mr.46.4.426-570.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roux K. H. Using mismatched primer-template pairs in touchdown PCR. Biotechniques. 1994 May;16(5):812–814. [PubMed] [Google Scholar]
  16. Sachs M. S., Yanofsky C. Developmental expression of genes involved in conidiation and amino acid biosynthesis in Neurospora crassa. Dev Biol. 1991 Nov;148(1):117–128. doi: 10.1016/0012-1606(91)90322-t. [DOI] [PubMed] [Google Scholar]
  17. Springer M. L. Genetic control of fungal differentiation: the three sporulation pathways of Neurospora crassa. Bioessays. 1993 Jun;15(6):365–374. doi: 10.1002/bies.950150602. [DOI] [PubMed] [Google Scholar]
  18. Springer M. L., Yanofsky C. A morphological and genetic analysis of conidiophore development in Neurospora crassa. Genes Dev. 1989 Apr;3(4):559–571. doi: 10.1101/gad.3.4.559. [DOI] [PubMed] [Google Scholar]
  19. Vollmer S. J., Yanofsky C. Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4869–4873. doi: 10.1073/pnas.83.13.4869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wieser J., Adams T. H. flbD encodes a Myb-like DNA-binding protein that coordinates initiation of Aspergillus nidulans conidiophore development. Genes Dev. 1995 Feb 15;9(4):491–502. doi: 10.1101/gad.9.4.491. [DOI] [PubMed] [Google Scholar]
  21. Wieser J., Lee B. N., Fondon J. w., 3rd, Adams T. H. Genetic requirements for initiating asexual development in Aspergillus nidulans. Curr Genet. 1994 Dec;27(1):62–69. doi: 10.1007/BF00326580. [DOI] [PubMed] [Google Scholar]
  22. Yamashiro C. T., Ebbole D. J., Lee B. U., Brown R. E., Bourland C., Madi L., Yanofsky C. Characterization of rco-1 of Neurospora crassa, a pleiotropic gene affecting growth and development that encodes a homolog of Tup1 of Saccharomyces cerevisiae. Mol Cell Biol. 1996 Nov;16(11):6218–6228. doi: 10.1128/mcb.16.11.6218. [DOI] [PMC free article] [PubMed] [Google Scholar]

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