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. 1998 Jul;149(3):1587–1597. doi: 10.1093/genetics/149.3.1587

Molecular characterization of S locus genes, SLG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica rapa L.

K Hatakeyama 1, T Takasaki 1, M Watanabe 1, K Hinata 1
PMCID: PMC1460250  PMID: 9649545

Abstract

In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.

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Selected References

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  1. Boutry M., Chua N. H. A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia. EMBO J. 1985 Sep;4(9):2159–2165. doi: 10.1002/j.1460-2075.1985.tb03910.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyes D. C., Chen C. H., Tantikanjana T., Esch J. J., Nasrallah J. B. Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci. Genetics. 1991 Jan;127(1):221–228. doi: 10.1093/genetics/127.1.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyes D. C., Nasrallah J. B. Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea. Mol Gen Genet. 1993 Jan;236(2-3):369–373. doi: 10.1007/BF00277135. [DOI] [PubMed] [Google Scholar]
  4. Cock J. M., Stanchev B., Delorme V., Croy R. R., Dumas C. SLR3: a modified receptor kinase gene that has been adapted to encode a putative secreted glycoprotein similar to the S locus glycoprotein. Mol Gen Genet. 1995 Jul 28;248(2):151–161. doi: 10.1007/BF02190796. [DOI] [PubMed] [Google Scholar]
  5. Delorme V., Giranton J. L., Hatzfeld Y., Friry A., Heizmann P., Ariza M. J., Dumas C., Gaude T., Cock J. M. Characterization of the S locus genes, SLG and SRK, of the Brassica S3 haplotype: identification of a membrane-localized protein encoded by the S locus receptor kinase gene. Plant J. 1995 Mar;7(3):429–440. doi: 10.1046/j.1365-313x.1995.7030429.x. [DOI] [PubMed] [Google Scholar]
  6. Dzelzkalns V. A., Thorsness M. K., Dwyer K. G., Baxter J. S., Balent M. A., Nasrallah M. E., Nasrallah J. B. Distinct cis-acting elements direct pistil-specific and pollen-specific activity of the Brassica S locus glycoprotein gene promoter. Plant Cell. 1993 Aug;5(8):855–863. doi: 10.1105/tpc.5.8.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hinata K., Watanabe M., Yamakawa S., Satta Y., Isogai A. Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system: synonymous and nonsynonymous base substitutions. Genetics. 1995 Jul;140(3):1099–1104. doi: 10.1093/genetics/140.3.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Isogai A., Yamakawa S., Shiozawa H., Takayama S., Tanaka H., Kono T., Watanabe M., Hinata K., Suzuki A. The cDNA sequence of NS1 glycoprotein of Brassica campestris and its homology to S-locus-related glycoproteins of B. oleracea. Plant Mol Biol. 1991 Aug;17(2):269–271. doi: 10.1007/BF00039503. [DOI] [PubMed] [Google Scholar]
  9. Lalonde B. A., Nasrallah M. E., Dwyer K. G., Chen C. H., Barlow B., Nasrallah J. B. A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene. Plant Cell. 1989 Feb;1(2):249–258. doi: 10.1105/tpc.1.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matsushita M., Watanabe M., Yamakawa S., Takayama S., Isogai A., Hinata K. The SLGs corresponding to the same S24-haplotype are perfectly conserved in three different self-incompatible Brassica campestris L. Genes Genet Syst. 1996 Aug;71(4):255–258. doi: 10.1266/ggs.71.255. [DOI] [PubMed] [Google Scholar]
  11. Murray M. G., Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980 Oct 10;8(19):4321–4325. doi: 10.1093/nar/8.19.4321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nasrallah J. B., Nasrallah M. E. Pollen[mdash]Stigma Signaling in the Sporophytic Self-Incompatibility Response. Plant Cell. 1993 Oct;5(10):1325–1335. doi: 10.1105/tpc.5.10.1325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Robert L. S., Allard S., Franklin T. M., Trick M. Sequence and expression of endogenous S-locus glycoprotein genes in self-compatible Brassica napus. Mol Gen Genet. 1994 Jan;242(2):209–216. doi: 10.1007/BF00391015. [DOI] [PubMed] [Google Scholar]
  15. Schierup M. H., Vekemans X., Christiansen F. B. Evolutionary dynamics of sporophytic self-incompatibility alleles in plants. Genetics. 1997 Oct;147(2):835–846. doi: 10.1093/genetics/147.2.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scutt C. P., Croy R. R. An S5 self-incompatibility allele-specific cDNA sequence from Brassica oleracea shows high homology to the SLR2 gene. Mol Gen Genet. 1992 Mar;232(2):240–246. doi: 10.1007/BF00280002. [DOI] [PubMed] [Google Scholar]
  17. Scutt C. P., Gates P. J., Gatehouse J. A., Boulter D., Croy R. R. A cDNA encoding an S-locus specific glycoprotein from Brassica oleracea plants containing the S5 self-incompatibility allele. Mol Gen Genet. 1990 Feb;220(3):409–413. doi: 10.1007/BF00391746. [DOI] [PubMed] [Google Scholar]
  18. Stein J. C., Howlett B., Boyes D. C., Nasrallah M. E., Nasrallah J. B. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8816–8820. doi: 10.1073/pnas.88.19.8816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Suzuki G., Watanabe M., Toriyama K., Isogai A., Hinata K. Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L. Plant Cell Physiol. 1995 Oct;36(7):1273–1280. [PubMed] [Google Scholar]
  20. Tantikanjana T., Nasrallah M. E., Stein J. C., Chen C. H., Nasrallah J. B. An alternative transcript of the S locus glycoprotein gene in a class II pollen-recessive self-incompatibility haplotype of Brassica oleracea encodes a membrane-anchored protein. Plant Cell. 1993 Jun;5(6):657–666. doi: 10.1105/tpc.5.6.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Trick M., Flavell R. B. A homozygous S genotype of Brassica oleracea expresses two S-like genes. Mol Gen Genet. 1989 Jul;218(1):112–117. doi: 10.1007/BF00330573. [DOI] [PubMed] [Google Scholar]
  22. Uyenoyama M. K. A generalized least-squares estimate for the origin of sporophytic self-incompatibility. Genetics. 1995 Feb;139(2):975–992. doi: 10.1093/genetics/139.2.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Watanabe M., Takasaki T., Toriyama K., Yamakawa S., Isogai A., Suzuki A., Hinata K. A high degree of homology exists between the protein encoded by SLG and the S receptor domain encoded by SRK in self-incompatible Brassica campestris L. Plant Cell Physiol. 1994 Dec;35(8):1221–1229. doi: 10.1093/oxfordjournals.pcp.a078716. [DOI] [PubMed] [Google Scholar]
  24. Yamakawa S., Shiba H., Watanabe M., Shiozawa H., Takayama S., Hinata K., Isogai A., Suzuki A. The sequences of S-glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae. Biosci Biotechnol Biochem. 1994 May;58(5):921–925. doi: 10.1271/bbb.58.921. [DOI] [PubMed] [Google Scholar]
  25. Yamakawa S., Watanabe M., Hinata K., Suzuki A., Isogai A. The sequences of S-receptor kinases (SRK) involved in self-incompatibility and their homologies to S-locus glycoproteins of Brassica campestris. Biosci Biotechnol Biochem. 1995 Jan;59(1):161–162. doi: 10.1271/bbb.59.161. [DOI] [PubMed] [Google Scholar]
  26. Yamakawa S., Watanabe M., Isogai A., Takayama S., Satoh S., Hinata K., Suzuki A. The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins. Plant Cell Physiol. 1993 Jan;34(1):173–175. [PubMed] [Google Scholar]

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