Abstract
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.
Full Text
The Full Text of this article is available as a PDF (46.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Golic K. G., Lindquist S. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell. 1989 Nov 3;59(3):499–509. doi: 10.1016/0092-8674(89)90033-0. [DOI] [PubMed] [Google Scholar]
- Gu H., Marth J. D., Orban P. C., Mossmann H., Rajewsky K. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science. 1994 Jul 1;265(5168):103–106. doi: 10.1126/science.8016642. [DOI] [PubMed] [Google Scholar]
- Gu H., Zou Y. R., Rajewsky K. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 1993 Jun 18;73(6):1155–1164. doi: 10.1016/0092-8674(93)90644-6. [DOI] [PubMed] [Google Scholar]
- Hamilton C. M., Aldea M., Washburn B. K., Babitzke P., Kushner S. R. New method for generating deletions and gene replacements in Escherichia coli. J Bacteriol. 1989 Sep;171(9):4617–4622. doi: 10.1128/jb.171.9.4617-4622.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jayaram M. Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5875–5879. doi: 10.1073/pnas.82.17.5875. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilby N. J., Snaith M. R., Murray J. A. Site-specific recombinases: tools for genome engineering. Trends Genet. 1993 Dec;9(12):413–421. doi: 10.1016/0168-9525(93)90104-p. [DOI] [PubMed] [Google Scholar]
- Kühn R., Schwenk F., Aguet M., Rajewsky K. Inducible gene targeting in mice. Science. 1995 Sep 8;269(5229):1427–1429. doi: 10.1126/science.7660125. [DOI] [PubMed] [Google Scholar]
- Logie C., Stewart A. F. Ligand-regulated site-specific recombination. Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):5940–5944. doi: 10.1073/pnas.92.13.5940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramírez-Solis R., Liu P., Bradley A. Chromosome engineering in mice. Nature. 1995 Dec 14;378(6558):720–724. doi: 10.1038/378720a0. [DOI] [PubMed] [Google Scholar]
- Rossant J., Nagy A. Genome engineering: the new mouse genetics. Nat Med. 1995 Jun;1(6):592–594. doi: 10.1038/nm0695-592. [DOI] [PubMed] [Google Scholar]
- Smith A. J., De Sousa M. A., Kwabi-Addo B., Heppell-Parton A., Impey H., Rabbitts P. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nat Genet. 1995 Apr;9(4):376–385. doi: 10.1038/ng0495-376. [DOI] [PubMed] [Google Scholar]