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. 1998 Dec;150(4):1605–1614. doi: 10.1093/genetics/150.4.1605

Abundant mitochondrial genome diversity, population differentiation and convergent evolution in pines.

J Wu 1, K V Krutovskii 1, S H Strauss 1
PMCID: PMC1460421  PMID: 9832536

Abstract

We examined mitochondrial DNA polymorphisms via the analysis of restriction fragment length polymorphisms in three closely related species of pines from western North America: knobcone (Pinus attenuata Lemm.), Monterey (P. radiata D. Don), and bishop (P. muricata D. Don). A total of 343 trees derived from 13 populations were analyzed using 13 homologous mitochondrial gene probes amplified from three species by polymerase chain reaction. Twenty-eight distinct mitochondrial DNA haplotypes were detected and no common haplotypes were found among the species. All three species showed limited variability within populations, but strong differentiation among populations. Based on haplotype frequencies, genetic diversity within populations (HS) averaged 0.22, and population differentiation (GST and theta) exceeded 0.78. Analysis of molecular variance also revealed that >90% of the variation resided among populations. For the purposes of genetic conservation and breeding programs, species and populations could be readily distinguished by unique haplotypes, often using the combination of only a few probes. Neighbor-joining phenograms, however, strongly disagreed with those based on allozymes, chloroplast DNA, and morphological traits. Thus, despite its diagnostic haplotypes, the genome appears to evolve via the rearrangement of multiple, convergent subgenomic domains.

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Selected References

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  1. Deu M., Hamon P., Chantereau J., Dufour P., D'hont A., Lanaud C. Mitochondrial DNA diversity in wild and cultivated sorghum. Genome. 1995 Aug;38(4):635–645. doi: 10.1139/g95-081. [DOI] [PubMed] [Google Scholar]
  2. Dong J., Wagner D. B. Paternally inherited chloroplast polymorphism in Pinus: estimation of diversity and population subdivision, and tests of disequilibrium with a maternally inherited mitochondrial polymorphism. Genetics. 1994 Mar;136(3):1187–1194. doi: 10.1093/genetics/136.3.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dumolin-Lapègue S., Demesure B., Fineschi S., Le Corre V., Petit R. J. Phylogeographic structure of white oaks throughout the European continent. Genetics. 1997 Aug;146(4):1475–1487. doi: 10.1093/genetics/146.4.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Excoffier L., Smouse P. E., Quattro J. M. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics. 1992 Jun;131(2):479–491. doi: 10.1093/genetics/131.2.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hiesel R., Combettes B., Brennicke A. Evidence for RNA editing in mitochondria of all major groups of land plants except the Bryophyta. Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):629–633. doi: 10.1073/pnas.91.2.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hong Y. P., Hipkins V. D., Strauss S. H. Chloroplast DNA diversity among trees, populations and species in the California closed-cone pines (Pinus radiata, Pinus muricata and Pinus attenuata). Genetics. 1993 Dec;135(4):1187–1196. doi: 10.1093/genetics/135.4.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hong Y. P., Krupkin A. B., Strauss S. H. Chloroplast DNA transgresses species boundaries and evolves at variable rates in the California closed-cone pines (Pinus radiata, P. muricata, and P. attenuata). Mol Phylogenet Evol. 1993 Dec;2(4):322–329. doi: 10.1006/mpev.1993.1031. [DOI] [PubMed] [Google Scholar]
  8. Maruyama T., Birky C. W., Jr Effects of periodic selection on gene diversity in organelle genomes and other systems without recombination. Genetics. 1991 Feb;127(2):449–451. doi: 10.1093/genetics/127.2.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Palmer J. D. Contrasting modes and tempos of genome evolution in land plant organelles. Trends Genet. 1990 Apr;6(4):115–120. doi: 10.1016/0168-9525(90)90125-p. [DOI] [PubMed] [Google Scholar]
  11. Perrotta G., Regina T. M., Ceci L. R., Quagliariello C. Conservation of the organization of the mitochondrial nad3 and rps12 genes in evolutionarily distant angiosperms. Mol Gen Genet. 1996 Jun 12;251(3):326–337. doi: 10.1007/BF02172523. [DOI] [PubMed] [Google Scholar]
  12. Wagner D. B., Furnier G. R., Saghai-Maroof M. A., Williams S. M., Dancik B. P., Allard R. W. Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. Proc Natl Acad Sci U S A. 1987 Apr;84(7):2097–2100. doi: 10.1073/pnas.84.7.2097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wolfe K. H., Li W. H., Sharp P. M. Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9054–9058. doi: 10.1073/pnas.84.24.9054. [DOI] [PMC free article] [PubMed] [Google Scholar]

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