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. 1999 Jan;151(1):331–341. doi: 10.1093/genetics/151.1.331

Functional analysis of deletion derivatives of the maize transposon MuDR delineates roles for the MURA and MURB proteins.

D Lisch 1, L Girard 1, M Donlin 1, M Freeling 1
PMCID: PMC1460458  PMID: 9872971

Abstract

The regulatory transposon of the Mutator system of transposable elements in maize is MuDR. MuDR elements produce two transcripts, from genes mudrA and mudrB, encoding proteins MURA and MURB, respectively. Like many other transposons, MuDR elements often undergo deletions, usually of internal sequences. Analysis of a deletion that is restricted to the region encoding MURB demonstrates that this gene is not required to cause excisions of a reporter element, although it may be required for transposition or suppression of suppressible alleles. Conversely, a derivative that lacks the region encoding MURA but that produces MURB is nonfunctional for all aspects of Mutator activity. Northern analysis of these derivatives reveals that each of the two transcripts can be independently transcribed, and analysis using an antibody specific for MURB reveals that mudrB transcript can also be successfully translated and its product appropriately localized in the absence of mudrA. A third deletion derivative provides evidence for a source of previously reported antisense transcript.

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Selected References

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  1. Alleman M., Freeling M. The Mu transposable elements of maize: evidence for transposition and copy number regulation during development. Genetics. 1986 Jan;112(1):107–119. doi: 10.1093/genetics/112.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benito M. I., Walbot V. Characterization of the maize Mutator transposable element MURA transposase as a DNA-binding protein. Mol Cell Biol. 1997 Sep;17(9):5165–5175. doi: 10.1128/mcb.17.9.5165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Black D. M., Jackson M. S., Kidwell M. G., Dover G. A. KP elements repress P-induced hybrid dysgenesis in Drosophila melanogaster. EMBO J. 1987 Dec 20;6(13):4125–4135. doi: 10.1002/j.1460-2075.1987.tb02758.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandler V. L., Hardeman K. J. The Mu elements of Zea mays. Adv Genet. 1992;30:77–122. doi: 10.1016/s0065-2660(08)60319-3. [DOI] [PubMed] [Google Scholar]
  5. Chomet P., Lisch D., Hardeman K. J., Chandler V. L., Freeling M. Identification of a regulatory transposon that controls the Mutator transposable element system in maize. Genetics. 1991 Sep;129(1):261–270. doi: 10.1093/genetics/129.1.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cocciolone S. M., Cone K. C. Pl-Bh, an anthocyanin regulatory gene of maize that leads to variegated pigmentation. Genetics. 1993 Oct;135(2):575–588. doi: 10.1093/genetics/135.2.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cuypers H., Dash S., Peterson P. A., Saedler H., Gierl A. The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays. EMBO J. 1988 Oct;7(10):2953–2960. doi: 10.1002/j.1460-2075.1988.tb03157.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Donlin M. J., Lisch D., Freeling M. Tissue-specific accumulation of MURB, a protein encoded by MuDR, the autonomous regulator of the Mutator transposable element family. Plant Cell. 1995 Dec;7(12):1989–2000. doi: 10.1105/tpc.7.12.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eisen J. A., Benito M. I., Walbot V. Sequence similarity of putative transposases links the maize Mutator autonomous element and a group of bacterial insertion sequences. Nucleic Acids Res. 1994 Jul 11;22(13):2634–2636. doi: 10.1093/nar/22.13.2634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fedoroff N., Wessler S., Shure M. Isolation of the transposable maize controlling elements Ac and Ds. Cell. 1983 Nov;35(1):235–242. doi: 10.1016/0092-8674(83)90226-x. [DOI] [PubMed] [Google Scholar]
  11. Fowler J. E., Muehlbauer G. J., Freeling M. Mosaic analysis of the liguleless3 mutant phenotype in maize by coordinate suppression of mutator-insertion alleles. Genetics. 1996 May;143(1):489–503. doi: 10.1093/genetics/143.1.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gloor G. B., Preston C. R., Johnson-Schlitz D. M., Nassif N. A., Phillis R. W., Benz W. K., Robertson H. M., Engels W. R. Type I repressors of P element mobility. Genetics. 1993 Sep;135(1):81–95. doi: 10.1093/genetics/135.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Greene B., Walko R., Hake S. Mutator insertions in an intron of the maize knotted1 gene result in dominant suppressible mutations. Genetics. 1994 Dec;138(4):1275–1285. doi: 10.1093/genetics/138.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hershberger R. J., Benito M. I., Hardeman K. J., Warren C., Chandler V. L., Walbot V. Characterization of the major transcripts encoded by the regulatory MuDR transposable element of maize. Genetics. 1995 Jul;140(3):1087–1098. doi: 10.1093/genetics/140.3.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hershberger R. J., Warren C. A., Walbot V. Mutator activity in maize correlates with the presence and expression of the Mu transposable element Mu9. Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10198–10202. doi: 10.1073/pnas.88.22.10198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hicks G. R., Smith H. M., Shieh M., Raikhel N. V. Three classes of nuclear import signals bind to plant nuclei. Plant Physiol. 1995 Apr;107(4):1055–1058. doi: 10.1104/pp.107.4.1055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hsia A. P., Schnable P. S. DNA sequence analyses support the role of interrupted gap repair in the origin of internal deletions of the maize transposon, MuDR. Genetics. 1996 Feb;142(2):603–618. doi: 10.1093/genetics/142.2.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. James M. G., Scanlon M. J., Qin M., Robertson D. S., Myers A. M. DNA sequence and transcript analysis of transposon MuA2, a regulator of Mutator transposable element activity in maize. Plant Mol Biol. 1993 Mar;21(6):1181–1185. doi: 10.1007/BF00023614. [DOI] [PubMed] [Google Scholar]
  19. Joanin P., Hershberger R. J., Benito M. I., Walbot V. Sense and antisense transcripts of the maize MuDR regulatory transposon localized by in situ hybridization. Plant Mol Biol. 1997 Jan;33(1):23–36. doi: 10.1023/a:1005790129668. [DOI] [PubMed] [Google Scholar]
  20. Kloeckener-Gruissem B., Vogel J. M., Freeling M. The TATA box promoter region of maize Adh1 affects its organ-specific expression. EMBO J. 1992 Jan;11(1):157–166. doi: 10.1002/j.1460-2075.1992.tb05038.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lisch D., Chomet P., Freeling M. Genetic characterization of the Mutator system in maize: behavior and regulation of Mu transposons in a minimal line. Genetics. 1995 Apr;139(4):1777–1796. doi: 10.1093/genetics/139.4.1777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Martienssen R., Barkan A., Taylor W. C., Freeling M. Somatically heritable switches in the DNA modification of Mu transposable elements monitored with a suppressible mutant in maize. Genes Dev. 1990 Mar;4(3):331–343. doi: 10.1101/gad.4.3.331. [DOI] [PubMed] [Google Scholar]
  23. Pohlman R. F., Fedoroff N. V., Messing J. The nucleotide sequence of the maize controlling element Activator. Cell. 1984 Jun;37(2):635–643. doi: 10.1016/0092-8674(84)90395-7. [DOI] [PubMed] [Google Scholar]
  24. Qin M. M., Robertson D. S., Ellingboe A. H. Cloning of the Mutator transposable element MuA2, a putative regulator of somatic mutability of the a1-Mum2 allele in maize. Genetics. 1991 Nov;129(3):845–854. doi: 10.1093/genetics/129.3.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rasmusson K. E., Raymond J. D., Simmons M. J. Repression of hybrid dysgenesis in Drosophila melanogaster by individual naturally occurring P elements. Genetics. 1993 Mar;133(3):605–622. doi: 10.1093/genetics/133.3.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Robertson H. M., Engels W. R. Modified P elements that mimic the P cytotype in Drosophila melanogaster. Genetics. 1989 Dec;123(4):815–824. doi: 10.1093/genetics/123.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Walbot V. The Mutator transposable element family of maize. Genet Eng (N Y) 1991;13:1–37. doi: 10.1007/978-1-4615-3760-1_1. [DOI] [PubMed] [Google Scholar]

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