Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1999 Mar;151(3):1005–1013. doi: 10.1093/genetics/151.3.1005

Transposition of the autonomous Fot1 element in the filamentous fungus Fusarium oxysporum.

Q Migheli 1, R Laugé 1, J M Davière 1, C Gerlinger 1, F Kaper 1, T Langin 1, M J Daboussi 1
PMCID: PMC1460518  PMID: 10049918

Abstract

Autonomous mobility of different copies of the Fot1 element was determined for several strains of the fungal plant pathogen Fusarium oxysporum to develop a transposon tagging system. Two Fot1 copies inserted into the third intron of the nitrate reductase structural gene (niaD) were separately introduced into two genetic backgrounds devoid of endogenous Fot1 elements. Mobility of these copies was observed through a phenotypic assay for excision based on the restoration of nitrate reductase activity. Inactivation of the Fot1 transposase open reading frame (frameshift, deletion, or disruption) prevented excision in strains free of Fot1 elements. Molecular analysis of the Nia+ revertant strains showed that the Fot1 element reintegrated frequently into new genomic sites after excision and that it can transpose from the introduced niaD gene into a different chromosome. Sequence analysis of several Fot1 excision sites revealed the so-called footprint left by this transposable element. Three reinserted Fot1 elements were cloned and the DNA sequences flanking the transposon were determined using inverse polymerase chain reaction. In all cases, the transposon was inserted into a TA dinucleotide and created the characteristic TA target site duplication. The availability of autonomous Fot1 copies will now permit the development of an efficient two-component transposon tagging system comprising a trans-activator element supplying transposase and a cis-responsive marked element.

Full Text

The Full Text of this article is available as a PDF (260.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aarts M. G., Dirkse W. G., Stiekema W. J., Pereira A. Transposon tagging of a male sterility gene in Arabidopsis. Nature. 1993 Jun 24;363(6431):715–717. doi: 10.1038/363715a0. [DOI] [PubMed] [Google Scholar]
  2. Atkinson P. W., Warren W. D., O'Brochta D. A. The hobo transposable element of Drosophila can be cross-mobilized in houseflies and excises like the Ac element of maize. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9693–9697. doi: 10.1073/pnas.90.20.9693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bancroft I., Bhatt A. M., Sjodin C., Scofield S., Jones J. D., Dean C. Development of an efficient two-element transposon tagging system in Arabidopsis thaliana. Mol Gen Genet. 1992 Jun;233(3):449–461. doi: 10.1007/BF00265443. [DOI] [PubMed] [Google Scholar]
  4. Bancroft I., Dean C. Transposition pattern of the maize element Ds in Arabidopsis thaliana. Genetics. 1993 Aug;134(4):1221–1229. doi: 10.1093/genetics/134.4.1221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bancroft I., Jones J. D., Dean C. Heterologous transposon tagging of the DRL1 locus in Arabidopsis. Plant Cell. 1993 Jun;5(6):631–638. doi: 10.1105/tpc.5.6.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chuck G., Robbins T., Nijjar C., Ralston E., Courtney-Gutterson N., Dooner H. K. Tagging and Cloning of a Petunia Flower Color Gene with the Maize Transposable Element Activator. Plant Cell. 1993 Apr;5(4):371–378. doi: 10.1105/tpc.5.4.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cooley L., Kelley R., Spradling A. Insertional mutagenesis of the Drosophila genome with single P elements. Science. 1988 Mar 4;239(4844):1121–1128. doi: 10.1126/science.2830671. [DOI] [PubMed] [Google Scholar]
  8. Cove D. J. Cholorate toxicity in Aspergillus nidulans: the selection and characterisation of chlorate resistant mutants. Heredity (Edinb) 1976 Apr;36(2):191–203. doi: 10.1038/hdy.1976.24. [DOI] [PubMed] [Google Scholar]
  9. Daboussi M. J., Djeballi A., Gerlinger C., Blaiseau P. L., Bouvier I., Cassan M., Lebrun M. H., Parisot D., Brygoo Y. Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans. Curr Genet. 1989 Jun;15(6):453–456. doi: 10.1007/BF00376803. [DOI] [PubMed] [Google Scholar]
  10. Daboussi M. J., Langin T., Brygoo Y. Fot1, a new family of fungal transposable elements. Mol Gen Genet. 1992 Mar;232(1):12–16. doi: 10.1007/BF00299131. [DOI] [PubMed] [Google Scholar]
  11. Dooner H. K., Belachew A. Transposition Pattern of the Maize Element Ac from the Bz-M2(ac) Allele. Genetics. 1989 Jun;122(2):447–457. doi: 10.1093/genetics/122.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dooner H. K., Keller J., Harper E., Ralston E. Variable Patterns of Transposition of the Maize Element Activator in Tobacco. Plant Cell. 1991 May;3(5):473–482. doi: 10.1105/tpc.3.5.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Eide D., Anderson P. Insertion and excision of Caenorhabditis elegans transposable element Tc1. Mol Cell Biol. 1988 Feb;8(2):737–746. doi: 10.1128/mcb.8.2.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Engels W. R., Johnson-Schlitz D. M., Eggleston W. B., Sved J. High-frequency P element loss in Drosophila is homolog dependent. Cell. 1990 Aug 10;62(3):515–525. doi: 10.1016/0092-8674(90)90016-8. [DOI] [PubMed] [Google Scholar]
  15. Farman M. L., Taura S., Leong S. A. The Magnaporthe grisea DNA fingerprinting probe MGR586 contains the 3' end of an inverted repeat transposon. Mol Gen Genet. 1996 Jul 26;251(6):675–681. doi: 10.1007/BF02174116. [DOI] [PubMed] [Google Scholar]
  16. Gierl A., Saedler H. Plant-transposable elements and gene tagging. Plant Mol Biol. 1992 May;19(1):39–49. doi: 10.1007/BF00015605. [DOI] [PubMed] [Google Scholar]
  17. Gloor G. B., Nassif N. A., Johnson-Schlitz D. M., Preston C. R., Engels W. R. Targeted gene replacement in Drosophila via P element-induced gap repair. Science. 1991 Sep 6;253(5024):1110–1117. doi: 10.1126/science.1653452. [DOI] [PubMed] [Google Scholar]
  18. Handler A. M., Gomez S. P. The hobo transposable element has transposase-dependent and -independent excision activity in drosophilid species. Mol Gen Genet. 1995 May 20;247(4):399–408. doi: 10.1007/BF00293140. [DOI] [PubMed] [Google Scholar]
  19. Honma M. A., Baker B. J., Waddell C. S. High-frequency germinal transposition of DsALS in Arabidopsis. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6242–6246. doi: 10.1073/pnas.90.13.6242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hyrien O., Debatisse M., Buttin G., de Saint Vincent B. R. The multicopy appearance of a large inverted duplication and the sequence at the inversion joint suggest a new model for gene amplification. EMBO J. 1988 Feb;7(2):407–417. doi: 10.1002/j.1460-2075.1988.tb02828.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Jones J. D., Carland F., Lim E., Ralston E., Dooner H. K. Preferential transposition of the maize element Activator to linked chromosomal locations in tobacco. Plant Cell. 1990 Aug;2(8):701–707. doi: 10.1105/tpc.2.8.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kachroo P., Leong S. A., Chattoo B. B. Pot2, an inverted repeat transposon from the rice blast fungus Magnaporthe grisea. Mol Gen Genet. 1994 Nov 1;245(3):339–348. doi: 10.1007/BF00290114. [DOI] [PubMed] [Google Scholar]
  23. Kinsey J. A., Helber J. Isolation of a transposable element from Neurospora crassa. Proc Natl Acad Sci U S A. 1989 Mar;86(6):1929–1933. doi: 10.1073/pnas.86.6.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Korswagen H. C., Durbin R. M., Smits M. T., Plasterk R. H. Transposon Tc1-derived, sequence-tagged sites in Caenorhabditis elegans as markers for gene mapping. Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14680–14685. doi: 10.1073/pnas.93.25.14680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Long D., Martin M., Sundberg E., Swinburne J., Puangsomlee P., Coupland G. The maize transposable element system Ac/Ds as a mutagen in Arabidopsis: identification of an albino mutation induced by Ds insertion. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10370–10374. doi: 10.1073/pnas.90.21.10370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Moerman D. G., Benian G. M., Waterston R. H. Molecular cloning of the muscle gene unc-22 in Caenorhabditis elegans by Tc1 transposon tagging. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2579–2583. doi: 10.1073/pnas.83.8.2579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Mäntylä A. L., Rossi K. H., Vanhanen S. A., Penttilä M. E., Suominen P. L., Nevalainen K. M. Electrophoretic karyotyping of wild-type and mutant Trichoderma longibrachiatum (reesei) strains. Curr Genet. 1992 May;21(6):471–477. doi: 10.1007/BF00351657. [DOI] [PubMed] [Google Scholar]
  28. Nyyssönen E., Amutan M., Enfield L., Stubbs J., Dunn-Coleman N. S. The transposable element Tan1 of Aspergillus niger var. awamori, a new member of the Fot1 family. Mol Gen Genet. 1996 Nov 27;253(1-2):50–56. doi: 10.1007/s004380050295. [DOI] [PubMed] [Google Scholar]
  29. Osborne B. I., Corr C. A., Prince J. P., Hehl R., Tanksley S. D., McCormick S., Baker B. Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome. Genetics. 1991 Nov;129(3):833–844. doi: 10.1093/genetics/129.3.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Panaccione D. G., Pitkin J. W., Walton J. D., Annis S. L. Transposon-like sequences at the TOX2 locus of the plant-pathogenic fungus Cochliobolus carbonum. Gene. 1996 Oct 17;176(1-2):103–109. doi: 10.1016/0378-1119(96)00228-4. [DOI] [PubMed] [Google Scholar]
  31. Paquin C. E., Dorsey M., Crable S., Sprinkel K., Sondej M., Williamson V. M. A spontaneous chromosomal amplification of the ADH2 gene in Saccharomyces cerevisiae. Genetics. 1992 Feb;130(2):263–271. doi: 10.1093/genetics/130.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Plasterk R. H., Groenen J. T. Targeted alterations of the Caenorhabditis elegans genome by transgene instructed DNA double strand break repair following Tc1 excision. EMBO J. 1992 Jan;11(1):287–290. doi: 10.1002/j.1460-2075.1992.tb05051.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Plasterk R. H. The origin of footprints of the Tc1 transposon of Caenorhabditis elegans. EMBO J. 1991 Jul;10(7):1919–1925. doi: 10.1002/j.1460-2075.1991.tb07718.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Punt P. J., Oliver R. P., Dingemanse M. A., Pouwels P. H., van den Hondel C. A. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene. 1987;56(1):117–124. doi: 10.1016/0378-1119(87)90164-8. [DOI] [PubMed] [Google Scholar]
  35. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Smit A. F., Riggs A. D. Tiggers and DNA transposon fossils in the human genome. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1443–1448. doi: 10.1073/pnas.93.4.1443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. van Luenen H. G., Plasterk R. H. Target site choice of the related transposable elements Tc1 and Tc3 of Caenorhabditis elegans. Nucleic Acids Res. 1994 Feb 11;22(3):262–269. doi: 10.1093/nar/22.3.262. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES