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. 1999 Mar;151(3):1165–1172. doi: 10.1093/genetics/151.3.1165

Temporal and multiple quantitative trait loci analyses of resistance to bacterial wilt in tomato permit the resolution of linked loci.

B Mangin 1, P Thoquet 1, J Olivier 1, N H Grimsley 1
PMCID: PMC1460533  PMID: 10049932

Abstract

Ralstonia solanacearum is a soil-borne bacterium that causes the serious disease known as bacterial wilt in many plant species. In tomato, several QTL controlling resistance have been found, but in different studies, markers spanning a large region of chromosome 6 showed strong association with the resistance. By using two different approaches to analyze the data from a field test F3 population, we show that at least two separate loci approximately 30 cM apart on this chromosome are most likely involved in the resistance. First, a temporal analysis of the progression of symptoms reveals a distal locus early in the development of the disease. As the disease progresses, the maximum LOD peak observed shifts toward the proximal end of the chromosome, obscuring the distal locus. Second, although classical interval mapping could only detect the presence of one locus, a statistical "two-QTL model" test, specifically adapted for the resolution of linked QTL, strongly supported the hypothesis for the presence of two loci. These results are discussed in the context of current molecular knowledge about disease resistance genes on chromosome 6 and observations made by tomato breeders during the production of bacterial wilt-resistant varieties.

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Selected References

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  1. Arlat M., Van Gijsegem F., Huet J. C., Pernollet J. C., Boucher C. A. PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum. EMBO J. 1994 Feb 1;13(3):543–553. doi: 10.1002/j.1460-2075.1994.tb06292.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker B., Zambryski P., Staskawicz B., Dinesh-Kumar S. P. Signaling in plant-microbe interactions. Science. 1997 May 2;276(5313):726–733. doi: 10.1126/science.276.5313.726. [DOI] [PubMed] [Google Scholar]
  3. Carney B. F., Denny T. P. A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level. J Bacteriol. 1990 Sep;172(9):4836–4843. doi: 10.1128/jb.172.9.4836-4843.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Danesh D., Aarons S., McGill G. E., Young N. D. Genetic dissection of oligogenic resistance to bacterial wilt in tomato. Mol Plant Microbe Interact. 1994 Jul-Aug;7(4):464–471. doi: 10.1094/mpmi-7-0464. [DOI] [PubMed] [Google Scholar]
  5. Dixon M. S., Jones D. A., Hatzixanthis K., Ganal M. W., Tanksley S. D., Jones J. D. High-resolution mapping of the physical location of the tomato Cf-2 gene. Mol Plant Microbe Interact. 1995 Mar-Apr;8(2):200–206. doi: 10.1094/mpmi-8-0200. [DOI] [PubMed] [Google Scholar]
  6. Dixon M. S., Jones D. A., Keddie J. S., Thomas C. M., Harrison K., Jones J. D. The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins. Cell. 1996 Feb 9;84(3):451–459. doi: 10.1016/s0092-8674(00)81290-8. [DOI] [PubMed] [Google Scholar]
  7. Doerge R. W., Churchill G. A. Permutation tests for multiple loci affecting a quantitative character. Genetics. 1996 Jan;142(1):285–294. doi: 10.1093/genetics/142.1.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haley C. S., Knott S. A. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity (Edinb) 1992 Oct;69(4):315–324. doi: 10.1038/hdy.1992.131. [DOI] [PubMed] [Google Scholar]
  9. Hammond-Kosack Kim E., Jones Jonathan D. G. PLANT DISEASE RESISTANCE GENES. Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48(NaN):575–607. doi: 10.1146/annurev.arplant.48.1.575. [DOI] [PubMed] [Google Scholar]
  10. Ho J. Y., Weide R., Ma H. M., van Wordragen M. F., Lambert K. N., Koornneef M., Zabel P., Williamson V. M. The root-knot nematode resistance gene (Mi) in tomato: construction of a molecular linkage map and identification of dominant cDNA markers in resistant genotypes. Plant J. 1992 Nov;2(6):971–982. [PubMed] [Google Scholar]
  11. Jansen R. C., Stam P. High resolution of quantitative traits into multiple loci via interval mapping. Genetics. 1994 Apr;136(4):1447–1455. doi: 10.1093/genetics/136.4.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kaloshian I., Lange W. H., Williamson V. M. An aphid-resistance locus is tightly linked to the nematode-resistance gene, Mi, in tomato. Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):622–625. doi: 10.1073/pnas.92.2.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  14. Mangin B., Goffinet B., Rebaï A. Constructing confidence intervals for QTL location. Genetics. 1994 Dec;138(4):1301–1308. doi: 10.1093/genetics/138.4.1301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Parniske M., Hammond-Kosack K. E., Golstein C., Thomas C. M., Jones D. A., Harrison K., Wulff B. B., Jones J. D. Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf-4/9 locus of tomato. Cell. 1997 Dec 12;91(6):821–832. doi: 10.1016/s0092-8674(00)80470-5. [DOI] [PubMed] [Google Scholar]
  16. Rebaï A., Goffinet B., Mangin B. Approximate thresholds of interval mapping tests for QTL detection. Genetics. 1994 Sep;138(1):235–240. doi: 10.1093/genetics/138.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Song W. Y., Pi L. Y., Wang G. L., Gardner J., Holsten T., Ronald P. C. Evolution of the rice Xa21 disease resistance gene family. Plant Cell. 1997 Aug;9(8):1279–1287. doi: 10.1105/tpc.9.8.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tanksley S. D., Ganal M. W., Prince J. P., de Vicente M. C., Bonierbale M. W., Broun P., Fulton T. M., Giovannoni J. J., Grandillo S., Martin G. B. High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992 Dec;132(4):1141–1160. doi: 10.1093/genetics/132.4.1141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weide R., van Wordragen M. F., Lankhorst R. K., Verkerk R., Hanhart C., Liharska T., Pap E., Stam P., Zabel P., Koornneef M. Integration of the classical and molecular linkage maps of tomato chromosome 6. Genetics. 1993 Dec;135(4):1175–1186. doi: 10.1093/genetics/135.4.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]

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