Abstract
Mutations affecting the self-incompatibility response of Nicotiana alata were generated by irradiation. Mutants in the M1 generation were selected on the basis of pollen tube growth through an otherwise incompatible pistil. Twelve of the 18 M1 plants obtained from the mutagenesis screen were self-compatible. Eleven self-compatible plants had mutations affecting only the pollen function of the S locus (pollen-part mutants). The remaining self-compatible plant had a mutation affecting only the style function of the S locus (style-part mutant). Cytological examination of the pollen-part mutant plants revealed that 8 had an extra chromosome (2n + 1) and 3 did not. The pollen-part mutation in 7 M1 plants was followed in a series of crosses. DNA blot analysis using probes for S-RNase genes (encoding the style function of the S locus) indicated that the pollen-part mutation was associated with an extra S allele in 4 M1 plants. In 3 of these plants, the extra S allele was located on the additional chromosome. There was no evidence of an extra S allele in the 3 remaining M1 plants. The breakdown of self-incompatibility in plants with an extra S allele is discussed with reference to current models of the molecular basis of self-incompatibility.
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Selected References
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- Anderson M. A., McFadden G. I., Bernatzky R., Atkinson A., Orpin T., Dedman H., Tregear G., Fernley R., Clarke A. E. Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata. Plant Cell. 1989 May;1(5):483–491. doi: 10.1105/tpc.1.5.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
- Brewbaker J L, Natarajan A T. Centric Fragments and Pollen-Part Mutation of Incompatibility Alleles in Petunia. Genetics. 1960 Jun;45(6):699–704. doi: 10.1093/genetics/45.6.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dodds P. N., Bönig I., Du H., Rödin J., Anderson M. A., Newbigin E., Clarke A. E. S-RNase gene of Nicotiana alata is expressed in developing pollen. Plant Cell. 1993 Dec;5(12):1771–1782. doi: 10.1105/tpc.5.12.1771. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lee H. S., Huang S., Kao T. S proteins control rejection of incompatible pollen in Petunia inflata. Nature. 1994 Feb 10;367(6463):560–563. doi: 10.1038/367560a0. [DOI] [PubMed] [Google Scholar]
- McClure B. A., Haring V., Ebert P. R., Anderson M. A., Simpson R. J., Sakiyama F., Clarke A. E. Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature. 1989 Dec 21;342(6252):955–957. doi: 10.1038/342955a0. [DOI] [PubMed] [Google Scholar]
- Thompson R. D., Kirch H. H. The S locus of flowering plants: when self-rejection is self-interest. Trends Genet. 1992 Nov;8(11):381–387. doi: 10.1016/0168-9525(92)90299-j. [DOI] [PubMed] [Google Scholar]
- Thompson R. D., Uhrig H., Hermsen J. G., Salamini F., Kaufmann H. Investigation of a self-compatible mutation in Solanum tuberosum clones inhibiting S-allele activity in pollen differentially. Mol Gen Genet. 1991 Apr;226(1-2):283–288. doi: 10.1007/BF00273614. [DOI] [PubMed] [Google Scholar]