Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Plasmid pAF1 (his3+) insertion mutation is in the git1+ complementation group. Diploid strains were constructed using intragenically complementing ade6-M210 and ade6-M216 mutant alleles to form Ade+ diploids. Diploid strains were spotted onto 5-FOA medium and grown for 3 days at 30°. 5-FOAR growth reflects the wild-type ability to glucose repress transcription of an fbp1-ura4+ reporter gene.
