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Complementation of a git1-1 mutation by plasmid-expressed git1+. Strains FWP72 (git1+) and CHP568 (git1-1) were transformed to Leu+ with pNMT41 (empty vector), pRSK3 (git1+), pRSK4 (git1-V5his6), or pRSK8 (git1ΔC2). Three independent transformants of each host strain and plasmid combination indicated in the figure were spotted to PM–leu and then replica plated after 2 days to either PM–leu or 5-FOA media. Plates were photographed after 3 days at 30°.
