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Git1 protein levels are regulated by glucose conditions. Western blot analysis of protein extracts from FWP72 (Git+) transformants carrying plasmid pRSK4 (Git1+-V5) or plasmid pRSK9 (Git1ΔC2-V5). Cells were grown under glucose-repressing (R) or derepressing (D; glucose-starved) conditions. Immunoblots were carried out using either α-V5 or α-Cdc2 (as a loading control) antibody as described in materials and methods.
