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. 2001 Jan;157(1):361–368. doi: 10.1093/genetics/157.1.361

Development and applications of a complete set of rice telotrisomics.

Z Cheng 1, H Yan 1, H Yu 1, S Tang 1, J Jiang 1, M Gu 1, L Zhu 1
PMCID: PMC1461484  PMID: 11139516

Abstract

We previously isolated a complete set of primary trisomics along with many other aneuploids from triploid plants derived from an indica rice variety "Zhongxian 3037." About 30,000 progeny from these trisomic and aneuploid plants were grown each year from 1994 to 1999. The variants that differed morphologically from both the diploids and the original primary trisomics were collected for cytological identification. From these variants, a complete set of telotrisomics covering all 24 rice chromosome arms was obtained. The identities of the extra chromosomes were further confirmed by dosage analysis of the RFLP markers on extra chromosome arms. The telocentric nature of the extra chromosomes in these stocks was verified by fluorescence in situ hybridization (FISH) using a rice centromeric BAC clone as a marker probe. In general, the shorter the extra chromosome arm of a telotrisomic, the stronger the resemblance it bears to the diploid; the longer the extra chromosome arm, the stronger the resemblance to the corresponding primary trisomic. We demonstrated that DNA clones can be rapidly assigned to specific chromosome arms by dosage analysis with the telotrisomics. We also showed that telotrisomics are valuable tools for chromosome microdissection and for developing chromosome-specific DNA markers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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