Skip to main content
PMC home page
Genetics logoLink to Genetics
. 2001 Oct;159(2):727–735. doi: 10.1093/genetics/159.2.727

Multiple quantitative trait locus analysis of bovine chromosome 6 in the Israeli Holstein population by a daughter design.

M Ron 1, D Kliger 1, E Feldmesser 1, E Seroussi 1, E Ezra 1, J I Weller 1
PMCID: PMC1461848  PMID: 11606547

Abstract

Nine Israeli Holstein sire families with 2978 daughters were analyzed for quantitative trait loci effects on chromosome 6 for five milk production traits by a daughter design. All animals were genotyped for 2 markers. The three families with significant effects were genotyped for up to 10 additional markers spanning positions 0-122 cM of BTA6. Two sires were segregating for a locus affecting protein and fat percentage near position 55 cM with an estimated substitution effect of 0.18% protein, which is equivalent to one phenotypic standard deviation. This locus was localized to a confidence interval of 4 cM. One of these sires was also heterozygous for a locus affecting milk, fat, and protein production near the centromere. The hypothesis of two segregating loci was verified by multiple regression analysis. A third sire was heterozygous for a locus affecting milk and protein percentage near the telomeric end of the chromosome. Possible candidates for the major quantitative gene near position 55 cM were determined by comparative mapping. IBSP and SSP1 were used as anchors for the orthologous region on human chromosome 4. Twelve genes were detected within a 2-Mbp sequence. None of these genes have been previously associated with lactogenesis.

Full Text

The Full Text of this article is available as a PDF (99.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Band M. R., Larson J. H., Rebeiz M., Green C. A., Heyen D. W., Donovan J., Windish R., Steining C., Mahyuddin P., Womack J. E. An ordered comparative map of the cattle and human genomes. Genome Res. 2000 Sep;10(9):1359–1368. doi: 10.1101/gr.145900. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darvasi A., Soller M. A simple method to calculate resolving power and confidence interval of QTL map location. Behav Genet. 1997 Mar;27(2):125–132. doi: 10.1023/a:1025685324830. [DOI] [PubMed] [Google Scholar]
  3. Georges M., Nielsen D., Mackinnon M., Mishra A., Okimoto R., Pasquino A. T., Sargeant L. S., Sorensen A., Steele M. R., Zhao X. Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing. Genetics. 1995 Feb;139(2):907–920. doi: 10.1093/genetics/139.2.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kühn C., Freyer G., Weikard R., Goldammer T., Schwerin M. Detection of QTL for milk production traits in cattle by application of a specifically developed marker map of BTA6. Anim Genet. 1999 Oct;30(5):333–340. doi: 10.1046/j.1365-2052.1999.00487.x. [DOI] [PubMed] [Google Scholar]
  5. Lipkin E., Mosig M. O., Darvasi A., Ezra E., Shalom A., Friedmann A., Soller M. Quantitative trait locus mapping in dairy cattle by means of selective milk DNA pooling using dinucleotide microsatellite markers: analysis of milk protein percentage. Genetics. 1998 Jul;149(3):1557–1567. doi: 10.1093/genetics/149.3.1557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ma R. Z., Beever J. E., Da Y., Green C. A., Russ I., Park C., Heyen D. W., Everts R. E., Fisher S. R., Overton K. M. A male linkage map of the cattle (Bos taurus) genome. J Hered. 1996 Jul-Aug;87(4):261–271. doi: 10.1093/oxfordjournals.jhered.a022999. [DOI] [PubMed] [Google Scholar]
  7. Nadesalingam J., Plante Y., Gibson J. P. Detection of QTL for milk production on Chromosomes 1 and 6 of Holstein cattle. Mamm Genome. 2001 Jan;12(1):27–31. doi: 10.1007/s003350010232. [DOI] [PubMed] [Google Scholar]
  8. Riquet J., Coppieters W., Cambisano N., Arranz J. J., Berzi P., Davis S. K., Grisart B., Farnir F., Karim L., Mni M. Fine-mapping of quantitative trait loci by identity by descent in outbred populations: application to milk production in dairy cattle. Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9252–9257. doi: 10.1073/pnas.96.16.9252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ron M., Blanc Y., Band M., Ezra E., Weller J. I. Misidentification rate in the Israeli dairy cattle population and its implications for genetic improvement. J Dairy Sci. 1996 Apr;79(4):676–681. doi: 10.3168/jds.S0022-0302(96)76413-5. [DOI] [PubMed] [Google Scholar]
  10. Rowe P. S., de Zoysa P. A., Dong R., Wang H. R., White K. E., Econs M. J., Oudet C. L. MEPE, a new gene expressed in bone marrow and tumors causing osteomalacia. Genomics. 2000 Jul 1;67(1):54–68. doi: 10.1006/geno.2000.6235. [DOI] [PubMed] [Google Scholar]
  11. Spelman R. J., Coppieters W., Karim L., van Arendonk J. A., Bovenhuis H. Quantitative trait loci analysis for five milk production traits on chromosome six in the Dutch Holstein-Friesian population. Genetics. 1996 Dec;144(4):1799–1808. doi: 10.1093/genetics/144.4.1799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Staden R., Beal K. F., Bonfield J. K. The Staden package, 1998. Methods Mol Biol. 2000;132:115–130. doi: 10.1385/1-59259-192-2:115. [DOI] [PubMed] [Google Scholar]
  13. Velmala R. J., Vilkki H. J., Elo K. T., de Koning D. J., Mäki-Tanila A. V. A search for quantitative trait loci for milk production traits on chromosome 6 in Finnish Ayrshire cattle. Anim Genet. 1999 Apr;30(2):136–143. doi: 10.1046/j.1365-2052.1999.00435.x. [DOI] [PubMed] [Google Scholar]
  14. Venter J. C., Adams M. D., Myers E. W., Li P. W., Mural R. J., Sutton G. G., Smith H. O., Yandell M., Evans C. A., Holt R. A. The sequence of the human genome. Science. 2001 Feb 16;291(5507):1304–1351. doi: 10.1126/science.1058040. [DOI] [PubMed] [Google Scholar]
  15. Visscher P. M., Thompson R., Haley C. S. Confidence intervals in QTL mapping by bootstrapping. Genetics. 1996 Jun;143(2):1013–1020. doi: 10.1093/genetics/143.2.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weller J. I., Kashi Y., Soller M. Power of daughter and granddaughter designs for determining linkage between marker loci and quantitative trait loci in dairy cattle. J Dairy Sci. 1990 Sep;73(9):2525–2537. doi: 10.3168/jds.S0022-0302(90)78938-2. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES