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. 2002 Jan;160(1):25–36. doi: 10.1093/genetics/160.1.25

Novel non-Mendelian determinant involved in the control of translation accuracy in Saccharomyces cerevisiae.

Kirill V Volkov 1, Anna Yu Aksenova 1, Malle J Soom 1, Kirill V Osipov 1, Anton V Svitin 1, Cornelia Kurischko 1, Irina S Shkundina 1, Michael D Ter-Avanesyan 1, Sergey G Inge-Vechtomov 1, Ludmila N Mironova 1
PMCID: PMC1461950  PMID: 11805042

Abstract

Two cytoplasmically inherited determinants related by their manifestation to the control of translation accuracy were previously described in yeast. Cells carrying one of them, [PSI(+)], display a nonsense suppressor phenotype and contain a prion form of the Sup35 protein. Another element, [PIN(+)], determines the probability of de novo generation of [PSI(+)] and results from a prion form of several proteins, which can be functionally unrelated to Sup35p. Here we describe a novel nonchromosomal determinant related to the SUP35 gene. This determinant, designated [ISP(+)], was identified as an antisuppressor of certain sup35 mutations. We observed its loss upon growth on guanidine hydrochloride and subsequent spontaneous reappearance with high frequency. The reversible curability of [ISP(+)] resembles the behavior of yeast prions. However, in contrast to known prions, [ISP(+)] does not depend on the chaperone protein Hsp104. Though manifestation of both [ISP(+)] and [PSI(+)] is related to the SUP35 gene, the maintenance of [ISP(+)] does not depend on the prionogenic N-terminal domain of Sup35p and Sup35p is not aggregated in [ISP(+)] cells, thus ruling out the possibility that [ISP(+)] is a specific form of [PSI(+)]. We hypothesize that [ISP(+)] is a novel prion involved in the control of translation accuracy in yeast.

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