Abstract
S. latifolia is a dioecious plant with morphologically distinct sex chromosomes. To genetically map the sex determination loci on the male-specific Y chromosome, we identified X-ray-induced sex determination mutants that had lost male traits. We used male-specific AFLP markers to characterize the extent of deletions in the Y chromosomes of the mutants. We then compared overlapping deletions to predict the order of the AFLP markers and to locate the mutated sex-determining genes. We found three regions on the Y chromosome where frequent deletions were significantly associated with loss of male traits. One was associated with hermaphroditic mutants. A second was associated with asexual mutants that lack genes needed for early stamen development and a third was associated with asexual mutants that lack genes for late stages of stamen development. Our observations confirmed a classical genetic prediction that S. latifolia has three dispersed male-determining loci on the Y chromosome, one for carpel suppression, one for early stamen development, and another for late stamen development. This AFLP map provides a framework for locating genes on the Y chromosome and for characterizing deletions on the Y chromosomes of potentially interesting mutants.
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Selected References
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- Boehnke M., Lange K., Cox D. R. Statistical methods for multipoint radiation hybrid mapping. Am J Hum Genet. 1991 Dec;49(6):1174–1188. [PMC free article] [PubMed] [Google Scholar]
- Bůzek J., Koutníková H., Houben A., Ríha K., Janousek B., Siroký J., Grant S., Vyskot B. Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album. Chromosome Res. 1997 Feb;5(1):57–65. doi: 10.1023/a:1011693603279. [DOI] [PubMed] [Google Scholar]
- Charlesworth B. Genome analysis: More Drosophila Y chromosome genes. Curr Biol. 2001 Mar 6;11(5):R182–R184. doi: 10.1016/s0960-9822(01)00089-6. [DOI] [PubMed] [Google Scholar]
- Charlesworth B. The evolution of sex chromosomes. Science. 1991 Mar 1;251(4997):1030–1033. doi: 10.1126/science.1998119. [DOI] [PubMed] [Google Scholar]
- Cox D. R., Burmeister M., Price E. R., Kim S., Myers R. M. Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. Science. 1990 Oct 12;250(4978):245–250. doi: 10.1126/science.2218528. [DOI] [PubMed] [Google Scholar]
- Desfeux C., Maurice S., Henry J. P., Lejeune B., Gouyon P. H. Evolution of reproductive systems in the genus Silene. Proc Biol Sci. 1996 Apr 22;263(1369):409–414. doi: 10.1098/rspb.1996.0062. [DOI] [PubMed] [Google Scholar]
- Donnison I. S., Siroky J., Vyskot B., Saedler H., Grant S. R. Isolation of Y chromosome-specific sequences from Silene latifolia and mapping of male sex-determining genes using representational difference analysis. Genetics. 1996 Dec;144(4):1893–1901. doi: 10.1093/genetics/144.4.1893. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Falk C. T. A simple method for ordering loci using data from radiation hybrids. Genomics. 1991 Jan;9(1):120–123. doi: 10.1016/0888-7543(91)90228-7. [DOI] [PubMed] [Google Scholar]
- Farbos I., Veuskens J., Vyskot B., Oliveira M., Hinnisdaels S., Aghmir A., Mouras A., Negrutiu I. Sexual dimorphism in white campion: deletion on the Y chromosome results in a floral asexual phenotype. Genetics. 1999 Mar;151(3):1187–1196. doi: 10.1093/genetics/151.3.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lahn B. T., Page D. C. Functional coherence of the human Y chromosome. Science. 1997 Oct 24;278(5338):675–680. doi: 10.1126/science.278.5338.675. [DOI] [PubMed] [Google Scholar]
- Lardon A., Georgiev S., Aghmir A., Le Merrer G., Negrutiu I. Sexual dimorphism in white campion: complex control of carpel number is revealed by y chromosome deletions. Genetics. 1999 Mar;151(3):1173–1185. doi: 10.1093/genetics/151.3.1173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lisitsyn N., Lisitsyn N., Wigler M. Cloning the differences between two complex genomes. Science. 1993 Feb 12;259(5097):946–951. doi: 10.1126/science.8438152. [DOI] [PubMed] [Google Scholar]
- Michelmore R. W., Paran I., Kesseli R. V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828–9832. doi: 10.1073/pnas.88.21.9828. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. doi: 10.1093/nar/23.21.4407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WESTERGAARD M. The mechanism of sex determination in dioecious flowering plants. Adv Genet. 1958;9:217–281. doi: 10.1016/s0065-2660(08)60163-7. [DOI] [PubMed] [Google Scholar]