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. 2002 Apr;160(4):1661–1671. doi: 10.1093/genetics/160.4.1661

Isolation and characterization of broad-spectrum disease-resistant Arabidopsis mutants.

Klaus Maleck 1, Urs Neuenschwander 1, Rebecca M Cade 1, Robert A Dietrich 1, Jeffery L Dangl 1, John A Ryals 1
PMCID: PMC1462068  PMID: 11973319

Abstract

To identify Arabidopsis mutants that constitutively express systemic acquired resistance (SAR), we constructed reporter lines expressing the firefly luciferase gene under the control of the SAR-inducible PR-1 promoter (PR-1/luc). After EMS mutagenesis of a well-characterized transgenic line, we screened 250,000 M(2) plants for constitutive expression of the reporter gene in vivo. From a mutant collection containing several hundred putative mutants, we concentrated on 16 mutants lacking spontaneous hypersensitive response (HR) cell death. We mapped 4 of these constitutive immunity (cim) mutants to chromosome arms. Constitutive expression of disease resistance was established by analyzing responses to virulent Peronospora parasitica and Pseudomonas syringae strains, by RNA blot analysis for endogenous marker genes, and by determination of salicylic acid levels in the mutants. The variety of the cim phenotypes allowed us to define distinct steps in both the canonical SAR signaling pathway and a separate pathway for resistance to Erysiphe cichoracearum, active in only a subset of the mutants.

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Selected References

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