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. 2002 Aug;161(4):1661–1672. doi: 10.1093/genetics/161.4.1661

Chromosomal map of the model legume Lotus japonicus.

Andrea Pedrosa 1, Niels Sandal 1, Jens Stougaard 1, Dieter Schweizer 1, Andreas Bachmair 1
PMCID: PMC1462223  PMID: 12196409

Abstract

Lotus japonicus is a model plant for the legume family. To facilitate map-based cloning approaches and genome analysis, we performed an extensive characterization of the chromosome complement of the species. A detailed karyotype of L. japonicus Gifu was built and plasmid and BAC clones, corresponding to genetically mapped markers (see the accompanying article by Sandal et al. 2002, this issue), were used for FISH to correlate genetic and chromosomal maps. Hybridization of DNA clones from 32 different genomic regions enabled the assignment of linkage groups to chromosomes, the comparison between genetic and physical distances throughout the genome, and the partial characterization of different repetitive sequences, including telomeric and centromeric repeats. Additional analysis of L. filicaulis and its F(1) hybrid with L. japonicus demonstrated the occurrence of inversions between these closely related species, suggesting that these chromosome rearrangements are early events in speciation of this group.

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Selected References

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