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. 2002 Sep;162(1):381–394. doi: 10.1093/genetics/162.1.381

Genetic and molecular characterization of the maize rp3 rust resistance locus.

Craig A Webb 1, Todd E Richter 1, Nicholas C Collins 1, Marie Nicolas 1, Harold N Trick 1, Tony Pryor 1, Scot H Hulbert 1
PMCID: PMC1462242  PMID: 12242248

Abstract

In maize, the Rp3 gene confers resistance to common rust caused by Puccinia sorghi. Flanking marker analysis of rust-susceptible rp3 variants suggested that most of them arose via unequal crossing over, indicating that rp3 is a complex locus like rp1. The PIC13 probe identifies a nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family that maps to the complex. Rp3 variants show losses of PIC13 family members relative to the resistant parents when probed with PIC13, indicating that the Rp3 gene is a member of this family. Gel blots and sequence analysis suggest that at least 9 family members are at the locus in most Rp3-carrying lines and that at least 5 of these are transcribed in the Rp3-A haplotype. The coding regions of 14 family members, isolated from three different Rp3-carrying haplotypes, had DNA sequence identities from 93 to 99%. Partial sequencing of clones of a BAC contig spanning the rp3 locus in the maize inbred line B73 identified five different PIC13 paralogues in a region of approximately 140 kb.

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Selected References

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