Abstract
We cloned a major aliphatic glucosinolate (GSL) gene, BoGSL-ELONG in Brassica oleracea, using the Arabidopsis sequence database. We based our work on an Arabidopsis candidate gene forming part of a gene family coding for isopropyl malate synthetase-like enzymes (IPMS). This gene is presumably responsible for synthesis of GSL possessing side chains consisting of four carbons (4C). The similarity of the Brassica homolog IPMS-Bo from broccoli to its Arabidopsis counterpart IPMS-At was on the order of 78%, both sharing the same number of exons. A nonfunctional allele of the BoGSL-ELONG gene from white cauliflower, based on the absence of 4C GSL in this crop, displayed a 30-bp deletion, which allowed us to develop a codominant marker for 4C-GSL. Gene expression analysis based on RT-PCR revealed a splicing site mutation in the white cauliflower allele. This resulted in a longer transcript containing intron 3, which failed to excise. Perfect cosegregation was observed for broccoli and cauliflower alleles at the IPMS-Bo gene and 4C-GSL content, strongly indicating that this gene indeed corresponds to BoGSL-ELONG. Cloning of two other major genes, BoGSL-ALK and BoGSL-PRO, is underway. The availability of these genes and BoGSL-ELONG is essential for the manipulation of the aliphatic GSL profile of B. oleracea.
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Selected References
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- Faulkner K., Mithen R., Williamson G. Selective increase of the potential anticarcinogen 4-methylsulphinylbutyl glucosinolate in broccoli. Carcinogenesis. 1998 Apr;19(4):605–609. doi: 10.1093/carcin/19.4.605. [DOI] [PubMed] [Google Scholar]
- Haughn G. W., Davin L., Giblin M., Underhill E. W. Biochemical Genetics of Plant Secondary Metabolites in Arabidopsis thaliana: The Glucosinolates. Plant Physiol. 1991 Sep;97(1):217–226. doi: 10.1104/pp.97.1.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kushad M. M., Brown A. F., Kurilich A. C., Juvik J. A., Klein B. P., Wallig M. A., Jeffery E. H. Variation of glucosinolates in vegetable crops of Brassica oleracea. J Agric Food Chem. 1999 Apr;47(4):1541–1548. doi: 10.1021/jf980985s. [DOI] [PubMed] [Google Scholar]
- Quiros C. F., Grellet F., Sadowski J., Suzuki T., Li G., Wroblewski T. Arabidopsis and Brassica comparative genomics: sequence, structure and gene content in the ABI-Rps2-Ck1 chromosomal segment and related regions. Genetics. 2001 Mar;157(3):1321–1330. doi: 10.1093/genetics/157.3.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]