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. 2002 Dec;162(4):1945–1959. doi: 10.1093/genetics/162.4.1945

Marker-assisted introgression of favorable alleles at quantitative trait loci between maize elite lines.

Agnès Bouchez 1, Frédéric Hospital 1, Mathilde Causse 1, André Gallais 1, Alain Charcosset 1
PMCID: PMC1462377  PMID: 12524362

Abstract

This article reports the marker-assisted introgression of favorable alleles at three quantitative trait loci (QTL) for earliness and grain yield among maize elite lines. The QTL were originally detected in 1992 by means of ANOVA in a population of 96 recombinant inbred lines (RILs). Introgression started from a selected RIL, which was crossed three times to one of the original parents and then self-fertilized, leading to BC(3)S(1) progenies. Markers were used to assist both foreground and background selection at each generation. At the end of the program, the effect of introgression was assessed phenotypically in agronomic trials, and QTL detection was performed by composite interval mapping among BC(3)S(1) progenies. The marker-assisted introgression proved successful at the genotypic level, as analyzed by precision graphical genotypes, although no emphasis was put on the reduction of linkage drag around QTL. Also, QTL positions were generally sustained in the introgression background. For earliness, the magnitude and sign of the QTL effects were in good agreement with those expected from initial RIL analyses. Conversely, for yield, important discrepancies were observed in the magnitude and sign of the QTL effects observed after introgression, when compared to those expected from initial RIL analyses. These discrepancies are probably due to important genotype-by-environment interactions.

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Selected References

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  1. Causse M., Santoni S., Damerval C., Maurice A., Charcosset A., Deatrick J., Vienne D. A composite map of expressed sequences in maize. Genome. 1996 Apr;39(2):418–432. doi: 10.1139/g96-053. [DOI] [PubMed] [Google Scholar]
  2. Dekkers Jack C. M., Hospital Frédéric. The use of molecular genetics in the improvement of agricultural populations. Nat Rev Genet. 2002 Jan;3(1):22–32. doi: 10.1038/nrg701. [DOI] [PubMed] [Google Scholar]
  3. Haley C. S., Knott S. A. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity (Edinb) 1992 Oct;69(4):315–324. doi: 10.1038/hdy.1992.131. [DOI] [PubMed] [Google Scholar]
  4. Hospital F., Charcosset A. Marker-assisted introgression of quantitative trait loci. Genetics. 1997 Nov;147(3):1469–1485. doi: 10.1093/genetics/147.3.1469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hospital F., Chevalet C., Mulsant P. Using markers in gene introgression breeding programs. Genetics. 1992 Dec;132(4):1199–1210. doi: 10.1093/genetics/132.4.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hospital F. Size of donor chromosome segments around introgressed loci and reduction of linkage drag in marker-assisted backcross programs. Genetics. 2001 Jul;158(3):1363–1379. doi: 10.1093/genetics/158.3.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jansen R. C., Stam P. High resolution of quantitative traits into multiple loci via interval mapping. Genetics. 1994 Apr;136(4):1447–1455. doi: 10.1093/genetics/136.4.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koudandé O. D., Iraqi F., Thomson P. C., Teale A. J., van Arendonk J. A. Strategies to optimize marker-assisted introgression of multiple unlinked QTL. Mamm Genome. 2000 Feb;11(2):145–150. doi: 10.1007/s003350010028. [DOI] [PubMed] [Google Scholar]
  9. Lander E. S., Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics. 1989 Jan;121(1):185–199. doi: 10.1093/genetics/121.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  11. Paterson A. H., DeVerna J. W., Lanini B., Tanksley S. D. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. Genetics. 1990 Mar;124(3):735–742. doi: 10.1093/genetics/124.3.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Servin Bertrand, Dillmann C., Decoux G., Hospital F. MDM: a program to compute fully informative genotype frequencies in complex breeding schemes. J Hered. 2002 May-Jun;93(3):227–228. doi: 10.1093/jhered/93.3.227. [DOI] [PubMed] [Google Scholar]
  13. Stuber C. W., Lincoln S. E., Wolff D. W., Helentjaris T., Lander E. S. Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics. 1992 Nov;132(3):823–839. doi: 10.1093/genetics/132.3.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Utz HF, Melchinger AE, Schön CC. Bias and Sampling Error of the Estimated Proportion of Genotypic Variance Explained by Quantitative Trait Loci Determined From Experimental Data in Maize Using Cross Validation and Validation With Independent Samples. Genetics. 2000 Apr;154(3):1839–1849. [PMC free article] [PubMed] [Google Scholar]
  15. Visscher P. M., Haley C. S., Thompson R. Marker-assisted introgression in backcross breeding programs. Genetics. 1996 Dec;144(4):1923–1932. doi: 10.1093/genetics/144.4.1923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vlăduţu C., McLaughlin J., Phillips R. L. Fine mapping and characterization of linked quantitative trait loci involved in the transition of the maize apical meristem from vegetative to generative structures. Genetics. 1999 Oct;153(2):993–1007. doi: 10.1093/genetics/153.2.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zeng Z. B. Precision mapping of quantitative trait loci. Genetics. 1994 Apr;136(4):1457–1468. doi: 10.1093/genetics/136.4.1457. [DOI] [PMC free article] [PubMed] [Google Scholar]

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