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. 2003 Mar;163(3):1221–1225. doi: 10.1093/genetics/163.3.1221

Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres.

Kiyotaka Nagaki 1, Paul B Talbert 1, Cathy Xiaoyan Zhong 1, R Kelly Dawe 1, Steven Henikoff 1, Jiming Jiang 1
PMCID: PMC1462492  PMID: 12663558

Abstract

The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

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Selected References

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