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. 2003 Jun;164(2):665–672. doi: 10.1093/genetics/164.2.665

A centromeric tandem repeat family originating from a part of Ty3/gypsy-retroelement in wheat and its relatives.

Zhi-Jun Cheng 1, Minoru Murata 1
PMCID: PMC1462596  PMID: 12807787

Abstract

From a wild diploid species that is a relative of wheat, Aegilops speltoides, a 301-bp repeat containing 16 copies of a CAA microsatellite was isolated. Southern blot and fluorescence in situ hybridization revealed that approximately 250 bp of the sequence is tandemly arrayed at the centromere regions of A- and B-genome chromosomes of common wheat and rye chromosomes. Although the DNA sequence of this 250-bp repeat showed no notable homology in the databases, the flanking or intervening sequences between the repeats showed high homologies (>82%) to two separate sequences of the gag gene and its upstream region in cereba, a Ty3/gypsy-like retroelement of Hordeum vulgare. Since the amino acid sequence deduced from the 250 bp with seven CAAs showed some similarity ( approximately 53%) to that of the gag gene, we concluded that the 250-bp repeats had also originated from the cereba-like retroelements in diploid wheat such as Ae. speltoides and had formed tandem arrays, whereas the 300-bp repeats were dispersed as a part of cereba-like retroelements. This suggests that some tandem repeats localized at the centromeric regions of cereals and other plant species originated from parts of retrotransposons.

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Selected References

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  1. Ananiev E. V., Phillips R. L., Rines H. W. Chromosome-specific molecular organization of maize (Zea mays L.) centromeric regions. Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13073–13078. doi: 10.1073/pnas.95.22.13073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arabidopsis Genome Initiative Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature. 2000 Dec 14;408(6814):796–815. doi: 10.1038/35048692. [DOI] [PubMed] [Google Scholar]
  3. Aragón-Alcaide L., Miller T., Schwarzacher T., Reader S., Moore G. A cereal centromeric sequence. Chromosoma. 1996 Dec;105(5):261–268. doi: 10.1007/BF02524643. [DOI] [PubMed] [Google Scholar]
  4. Bedbrook J. R., Jones J., O'Dell M., Thompson R. D., Flavell R. B. A molecular description of telometic heterochromatin in secale species. Cell. 1980 Feb;19(2):545–560. doi: 10.1016/0092-8674(80)90529-2. [DOI] [PubMed] [Google Scholar]
  5. Brandes A., Thompson H., Dean C., Heslop-Harrison J. S. Multiple repetitive DNA sequences in the paracentromeric regions of Arabidopsis thaliana L. Chromosome Res. 1997 Jun;5(4):238–246. doi: 10.1023/a:1018415502795. [DOI] [PubMed] [Google Scholar]
  6. Cheng Zhi-Jun, Murata Minoru. Loss of chromosomes 2R and 5RS in octoploid triticale selected for agronomic traits. Genes Genet Syst. 2002 Feb;77(1):23–29. doi: 10.1266/ggs.77.23. [DOI] [PubMed] [Google Scholar]
  7. Cheng Zhukuan, Dong Fenggao, Langdon Tim, Ouyang Shu, Buell C. Robin, Gu Minghong, Blattner Frederick R., Jiang Jiming. Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon. Plant Cell. 2002 Aug;14(8):1691–1704. doi: 10.1105/tpc.003079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Copenhaver G. P., Nickel K., Kuromori T., Benito M. I., Kaul S., Lin X., Bevan M., Murphy G., Harris B., Parnell L. D. Genetic definition and sequence analysis of Arabidopsis centromeres. Science. 1999 Dec 24;286(5449):2468–2474. doi: 10.1126/science.286.5449.2468. [DOI] [PubMed] [Google Scholar]
  9. Dong F., Miller J. T., Jackson S. A., Wang G. L., Ronald P. C., Jiang J. Rice (Oryza sativa) centromeric regions consist of complex DNA. Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8135–8140. doi: 10.1073/pnas.95.14.8135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dvorák J., Zhang H. B. Variation in repeated nucleotide sequences sheds light on the phylogeny of the wheat B and G genomes. Proc Natl Acad Sci U S A. 1990 Dec 15;87(24):9640–9644. doi: 10.1073/pnas.87.24.9640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fukui K. N., Suzuki G., Lagudah E. S., Rahman S., Appels R., Yamamoto M., Mukai Y. Physical arrangement of retrotransposon-related repeats in centromeric regions of wheat. Plant Cell Physiol. 2001 Feb;42(2):189–196. doi: 10.1093/pcp/pce026. [DOI] [PubMed] [Google Scholar]
  12. Hudakova S., Michalek W., Presting G. G., ten Hoopen R., dos Santos K., Jasencakova Z., Schubert I. Sequence organization of barley centromeres. Nucleic Acids Res. 2001 Dec 15;29(24):5029–5035. doi: 10.1093/nar/29.24.5029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jiang J., Nasuda S., Dong F., Scherrer C. W., Woo S. S., Wing R. A., Gill B. S., Ward D. C. A conserved repetitive DNA element located in the centromeres of cereal chromosomes. Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):14210–14213. doi: 10.1073/pnas.93.24.14210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kishii M., Nagaki K., Tsujimoto H. A tandem repetitive sequence located in the centromeric region of common wheat (Triticum aestivum) chromosomes. Chromosome Res. 2001;9(5):417–428. doi: 10.1023/a:1016739719421. [DOI] [PubMed] [Google Scholar]
  15. Langdon T., Seago C., Mende M., Leggett M., Thomas H., Forster J. W., Jones R. N., Jenkins G. Retrotransposon evolution in diverse plant genomes. Genetics. 2000 Sep;156(1):313–325. doi: 10.1093/genetics/156.1.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miller J. T., Dong F., Jackson S. A., Song J., Jiang J. Retrotransposon-related DNA sequences in the centromeres of grass chromosomes. Genetics. 1998 Dec;150(4):1615–1623. doi: 10.1093/genetics/150.4.1615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Murata M., Ogura Y., Motoyoshi F. Centromeric repetitive sequences in Arabidopsis thaliana. Jpn J Genet. 1994 Aug;69(4):361–370. doi: 10.1266/jjg.69.361. [DOI] [PubMed] [Google Scholar]
  18. Nagaki K., Tsujimoto H., Sasakuma T. A novel repetitive sequence of sugar cane, SCEN family, locating on centromeric regions. Chromosome Res. 1998 Jun;6(4):295–302. doi: 10.1023/a:1009270824142. [DOI] [PubMed] [Google Scholar]
  19. Presting G. G., Malysheva L., Fuchs J., Schubert I. A Ty3/gypsy retrotransposon-like sequence localizes to the centromeric regions of cereal chromosomes. Plant J. 1998 Dec;16(6):721–728. doi: 10.1046/j.1365-313x.1998.00341.x. [DOI] [PubMed] [Google Scholar]
  20. Schlötterer C. Evolutionary dynamics of microsatellite DNA. Chromosoma. 2000 Sep;109(6):365–371. doi: 10.1007/s004120000089. [DOI] [PubMed] [Google Scholar]
  21. Schmidt T., Heslop-Harrison J. S. The physical and genomic organization of microsatellites in sugar beet. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8761–8765. doi: 10.1073/pnas.93.16.8761. [DOI] [PMC free article] [PubMed] [Google Scholar]

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