Skip to main content
PMC home page
Genetics logoLink to Genetics
. 2003 Oct;165(2):929–934. doi: 10.1093/genetics/165.2.929

A function for subtelomeric DNA in Saccharomyces cerevisiae.

Arnold B Barton 1, Yuping Su 1, Jacque Lamb 1, Dianna Barber 1, David B Kaback 1
PMCID: PMC1462788  PMID: 14573499

Abstract

The subtelomeric DNA sequences from chromosome I of Saccharomyces cerevisiae are shown to be inherently poor substrates for meiotic recombination. On the basis of these results and prior observations that crossovers near telomeres do not promote efficient meiosis I segregation, we suggest that subtelomeric sequences evolved to prevent recombination from occurring where it cannot promote efficient segregation.

Full Text

The Full Text of this article is available as a PDF (156.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barton A. B., Bussey H., Storms R. K., Kaback D. B. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: characterization of the 54 kb right terminal CDC15-FLO1-PHO11 region. Yeast. 1997 Oct;13(13):1251–1263. doi: 10.1002/(sici)1097-0061(199710)13:13<1251::aid-yea174>3.3.co;2-6. [DOI] [PubMed] [Google Scholar]
  2. Carpenter A. T. A meiotic mutant defective in distributive disjunction in Drosophila melanogaster. Genetics. 1973 Mar;73(3):393–428. doi: 10.1093/genetics/73.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ferguson B. M., Fangman W. L. A position effect on the time of replication origin activation in yeast. Cell. 1992 Jan 24;68(2):333–339. doi: 10.1016/0092-8674(92)90474-q. [DOI] [PubMed] [Google Scholar]
  4. Gerton J. L., DeRisi J., Shroff R., Lichten M., Brown P. O., Petes T. D. Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11383–11390. doi: 10.1073/pnas.97.21.11383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldman A. S., Lichten M. The efficiency of meiotic recombination between dispersed sequences in Saccharomyces cerevisiae depends upon their chromosomal location. Genetics. 1996 Sep;144(1):43–55. doi: 10.1093/genetics/144.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gottschling D. E., Aparicio O. M., Billington B. L., Zakian V. A. Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription. Cell. 1990 Nov 16;63(4):751–762. doi: 10.1016/0092-8674(90)90141-z. [DOI] [PubMed] [Google Scholar]
  7. Kaback D. B., Steensma H. Y., de Jonge P. Enhanced meiotic recombination on the smallest chromosome of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1989 May;86(10):3694–3698. doi: 10.1073/pnas.86.10.3694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klein S., Zenvirth D., Dror V., Barton A. B., Kaback D. B., Simchen G. Patterns of meiotic double-strand breakage on native and artificial yeast chromosomes. Chromosoma. 1996 Dec;105(5):276–284. doi: 10.1007/BF02524645. [DOI] [PubMed] [Google Scholar]
  9. Louis E. J. The chromosome ends of Saccharomyces cerevisiae. Yeast. 1995 Dec;11(16):1553–1573. doi: 10.1002/yea.320111604. [DOI] [PubMed] [Google Scholar]
  10. Moore D. P., Miyazaki W. Y., Tomkiel J. E., Orr-Weaver T. L. Double or nothing: a Drosophila mutation affecting meiotic chromosome segregation in both females and males. Genetics. 1994 Mar;136(3):953–964. doi: 10.1093/genetics/136.3.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mortimer R. K., Schild D., Contopoulou C. R., Kans J. A. Genetic map of Saccharomyces cerevisiae, edition 10. Yeast. 1989 Sep-Oct;5(5):321–403. doi: 10.1002/yea.320050503. [DOI] [PubMed] [Google Scholar]
  12. Mortimer R. K., Schild D. Genetic map of Saccharomyces cerevisiae, edition 9. Microbiol Rev. 1985 Sep;49(3):181–213. doi: 10.1128/mr.49.3.181-213.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mortimer R. K., Schild D. Genetic map of Saccharomyces cerevisiae. Microbiol Rev. 1980 Dec;44(4):519–571. doi: 10.1128/mr.44.4.519-571.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pryde F. E., Louis E. J. Saccharomyces cerevisiae telomeres. A review. Biochemistry (Mosc) 1997 Nov;62(11):1232–1241. [PubMed] [Google Scholar]
  15. Rasooly R. S., New C. M., Zhang P., Hawley R. S., Baker B. S. The lethal(1)TW-6cs mutation of Drosophila melanogaster is a dominant antimorphic allele of nod and is associated with a single base change in the putative ATP-binding domain. Genetics. 1991 Oct;129(2):409–422. doi: 10.1093/genetics/129.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Renauld H., Aparicio O. M., Zierath P. D., Billington B. L., Chhablani S. K., Gottschling D. E. Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage. Genes Dev. 1993 Jul;7(7A):1133–1145. doi: 10.1101/gad.7.7a.1133. [DOI] [PubMed] [Google Scholar]
  17. Ross L. O., Maxfield R., Dawson D. Exchanges are not equally able to enhance meiotic chromosome segregation in yeast. Proc Natl Acad Sci U S A. 1996 May 14;93(10):4979–4983. doi: 10.1073/pnas.93.10.4979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Stavenhagen J. B., Zakian V. A. Yeast telomeres exert a position effect on recombination between internal tracts of yeast telomeric DNA. Genes Dev. 1998 Oct 1;12(19):3044–3058. doi: 10.1101/gad.12.19.3044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Trelles-Sticken E., Loidl J., Scherthan H. Bouquet formation in budding yeast: initiation of recombination is not required for meiotic telomere clustering. J Cell Sci. 1999 Mar;112(Pt 5):651–658. doi: 10.1242/jcs.112.5.651. [DOI] [PubMed] [Google Scholar]
  22. Wyrick J. J., Holstege F. C., Jennings E. G., Causton H. C., Shore D., Grunstein M., Lander E. S., Young R. A. Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast. Nature. 1999 Nov 25;402(6760):418–421. doi: 10.1038/46567. [DOI] [PubMed] [Google Scholar]
  23. Yang C., Theis J. F., Newlon C. S. Conservation of ARS elements and chromosomal DNA replication origins on chromosomes III of Saccharomyces cerevisiae and S. carlsbergensis. Genetics. 1999 Jul;152(3):933–941. doi: 10.1093/genetics/152.3.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. de Steensma H. Y., de Jonge P., Kaptein A., Kaback D. B. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: localization of a repeated sequence containing an acid phosphatase gene near a telomere of chromosome I and chromosome VIII. Curr Genet. 1989 Sep;16(3):131–137. doi: 10.1007/BF00391468. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES