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. 1996 Dec 1;24(23):4838–4840. doi: 10.1093/nar/24.23.4838

A tri-hybrid system for the analysis and detection of RNA--protein interactions.

U Putz 1, P Skehel 1, D Kuhl 1
PMCID: PMC146291  PMID: 8972875

Abstract

A modification of the two-hybrid system is described for the in vivo reconstruction of specific RNA-protein interactions. In this tri-hybrid system, the DNA binding and transcription activation domains of the yeast transcriptional activator GAL4 are brought together via the interaction of recombinant fusion proteins with a recombinant RNA. The method provides a system for studying RNA-protein interactions with the genetic advantages of the two-hybrid system. It may be used to detect specific RNA-binding proteins or target RNAs from a library of cDNAs, or to analyse the structural specificity of identified RNA-protein interactions.

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Selected References

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