Abstract
With the phosphatase inhibitor, okadaic acid, we induce the precocious onset of the chiasmate stage and under those conditions show that the recombination nodules, MLH1 and MLH3 foci, are localized to the chiasmata. It is concluded that MLH1/3 foci are appropriate markers for the studies of crossovers/chiasmata development and distribution at late meiotic prophase.
Full Text
The Full Text of this article is available as a PDF (303.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Albini S. M., Jones G. H. Synaptonemal complex-associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique. Exp Cell Res. 1984 Dec;155(2):588–592. doi: 10.1016/0014-4827(84)90219-2. [DOI] [PubMed] [Google Scholar]
- Anderson L. K., Reeves A., Webb L. M., Ashley T. Distribution of crossing over on mouse synaptonemal complexes using immunofluorescent localization of MLH1 protein. Genetics. 1999 Apr;151(4):1569–1579. doi: 10.1093/genetics/151.4.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baker S. M., Plug A. W., Prolla T. A., Bronner C. E., Harris A. C., Yao X., Christie D. M., Monell C., Arnheim N., Bradley A. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat Genet. 1996 Jul;13(3):336–342. doi: 10.1038/ng0796-336. [DOI] [PubMed] [Google Scholar]
- Barlow A. L., Hultén M. A. Crossing over analysis at pachytene in man. Eur J Hum Genet. 1998 Jul-Aug;6(4):350–358. doi: 10.1038/sj.ejhg.5200200. [DOI] [PubMed] [Google Scholar]
- Carpenter A. T. Electron microscopy of meiosis in Drosophila melanogaster females: II. The recombination nodule--a recombination-associated structure at pachytene? Proc Natl Acad Sci U S A. 1975 Aug;72(8):3186–3189. doi: 10.1073/pnas.72.8.3186. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carpenter A. T. Recombination nodules and synaptonemal complex in recombination-defective females of Drosophila melanogaster. Chromosoma. 1979;75(3):259–292. doi: 10.1007/BF00293472. [DOI] [PubMed] [Google Scholar]
- Carpenter A. T. Synaptonemal complex and recombination nodules in wild-type Drosophila melanogaster females. Genetics. 1979 Jun;92(2):511–541. doi: 10.1093/genetics/92.2.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dobson M. J., Pearlman R. E., Karaiskakis A., Spyropoulos B., Moens P. B. Synaptonemal complex proteins: occurrence, epitope mapping and chromosome disjunction. J Cell Sci. 1994 Oct;107(Pt 10):2749–2760. doi: 10.1242/jcs.107.10.2749. [DOI] [PubMed] [Google Scholar]
- Gillies C. B. The Relationship between Synaptinemal Complexes, Recombination Nodules and Crossing over in NEUROSPORA CRASSA Bivalents and Translocation Quadrivalents. Genetics. 1979 Jan;91(1):1–17. doi: 10.1093/genetics/91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hassold T., Sherman S., Hunt P. Counting cross-overs: characterizing meiotic recombination in mammals. Hum Mol Genet. 2000 Oct;9(16):2409–2419. doi: 10.1093/hmg/9.16.2409. [DOI] [PubMed] [Google Scholar]
- Heyting C., Dietrich A. J., Redeker E. J., Vink A. C. Structure and composition of synaptonemal complexes, isolated from rat spermatocytes. Eur J Cell Biol. 1985 Mar;36(2):307–314. [PubMed] [Google Scholar]
- Kanda N., Kato H. Analysis of crossing over in mouse meiotic cells by BrdU labelling technique. Chromosoma. 1980;78(1):113–121. doi: 10.1007/BF00291910. [DOI] [PubMed] [Google Scholar]
- Koehler Kara E., Cherry Jonathan P., Lynn Audrey, Hunt Patricia A., Hassold Terry J. Genetic control of mammalian meiotic recombination. I. Variation in exchange frequencies among males from inbred mouse strains. Genetics. 2002 Sep;162(1):297–306. doi: 10.1093/genetics/162.1.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipkin Steven M., Moens Peter B., Wang Victoria, Lenzi Michelle, Shanmugarajah Dakshine, Gilgeous Abigail, Thomas James, Cheng Jun, Touchman Jeffrey W., Green Eric D. Meiotic arrest and aneuploidy in MLH3-deficient mice. Nat Genet. 2002 Jul 1;31(4):385–390. doi: 10.1038/ng931. [DOI] [PubMed] [Google Scholar]
- Lynn Audrey, Koehler Kara E., Judis LuAnn, Chan Ernest R., Cherry Jonathan P., Schwartz Stuart, Seftel Allen, Hunt Patricia A., Hassold Terry J. Covariation of synaptonemal complex length and mammalian meiotic exchange rates. Science. 2002 Jun 6;296(5576):2222–2225. doi: 10.1126/science.1071220. [DOI] [PubMed] [Google Scholar]
- Moens P. B., Spyropoulos B. Immunocytology of chiasmata and chromosomal disjunction at mouse meiosis. Chromosoma. 1995 Nov;104(3):175–182. doi: 10.1007/BF00352182. [DOI] [PubMed] [Google Scholar]
- Moens Peter B., Kolas Nadine K., Tarsounas Madalena, Marcon Edyta, Cohen Paula E., Spyropoulos Barbara. The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination. J Cell Sci. 2002 Apr 15;115(Pt 8):1611–1622. doi: 10.1242/jcs.115.8.1611. [DOI] [PubMed] [Google Scholar]
- Tarsounas M., Pearlman R. E., Moens P. B. Meiotic activation of rat pachytene spermatocytes with okadaic acid: the behaviour of synaptonemal complex components SYN1/SCP1 and COR1/SCP3. J Cell Sci. 1999 Feb;112(Pt 4):423–434. doi: 10.1242/jcs.112.4.423. [DOI] [PubMed] [Google Scholar]
- Wiltshire T., Park C., Caldwell K. A., Handel M. A. Induced premature G2/M-phase transition in pachytene spermatocytes includes events unique to meiosis. Dev Biol. 1995 Jun;169(2):557–567. doi: 10.1006/dbio.1995.1169. [DOI] [PubMed] [Google Scholar]
- Zickler D. Development of the synaptonemal complex and the "recombination nodules" during meiotic prophase in the seven bivalents of the fungus Sordaria macrospora Auersw. Chromosoma. 1977 Jun 23;61(4):289–316. doi: 10.1007/BF00288615. [DOI] [PubMed] [Google Scholar]