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. Author manuscript; available in PMC: 2006 May 23.
Published in final edited form as: Nature. 2005 Aug 4;436(7051):714–719. doi: 10.1038/nature03863

Figure 3.

Figure 3

Molecular analysis of tga1. a. Top panel is a Northern blot showing that tga1 is expressed in immature ears of W22 maize (M) and W22:tga1 (T) which carries the teosinte allele. Weak expression is also visible in the husks (HK). No expression of tga1 is seen in root (RT), prop root (PR), unexpanded leaf (LF) or immature tassel (TS). Lower panel is the ethidium bromide stained gel with the visible rRNAs confirming approximately equal loading of total RNA in each lane. b. Western blots showing that the protein encoded by the teosinte allele of tga1 accumulates at a higher level than the protein of the maize allele. Blots probed with either anti-TGA1 or anti-ACTIN (control) antibodies. Protein was extracted from individual ears of W22 maize (M) carrying a maize allele and W22:tga1 (T) carrying a teosinte allele. Immature ears were staged by their lengths which are indicated in mm.