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. 1997 Feb 1;25(3):568–574. doi: 10.1093/nar/25.3.568

Evaluation of pyrimidine PNA binding to ssDNA targets from nonequilibrium melting experiments.

E A Lesnik 1, L M Risen 1, D A Driver 1, M C Griffith 1, K Sprankle 1, S M Freier 1
PMCID: PMC146474  PMID: 9016597

Abstract

Slow kinetics of homopyrimidine PNA binding to single stranded DNA and RNA targets is manifested in significant hysteresis in thermal UV absorption experiments. We have compared temperatures of dissociation (Tdis) and reassociation (Tass) for triplexes formed by DNA and single or bis PNAs with K50 derived from gel mobility experiments. Results indicated there was no correlation between Tdis and K50 while reasonable correlation between Tass and K50 was found. This correlation enabled use of easy thermal UV absorption experiments for evaluation of PNA binding to DNA/RNA targets.

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Selected References

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