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. 1997 Feb 15;25(4):713–718. doi: 10.1093/nar/25.4.713

DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter.

D Nègre 1, C Bonod-Bidaud 1, C Oudot 1, J F Prost 1, A Kolb 1, A Ishihama 1, A J Cozzone 1, J C Cortay 1
PMCID: PMC146490  PMID: 9016619

Abstract

The specific interaction of the upstream element-containing promoter of the Escherichia coli acetate operon with either the RNA polymerase holoenzyme or its alpha subunit has been analyzed by the base removal method. Our results indicate that: (i) direct and specific base contacts can be detected in the acetate promoter-alpha subunit complex; (ii) base elimination in the upstream element of the acetate promoter enhances the binding of RNA polymerase. A similar effect is observed when studying the interactions between RNA polymerase and the rrnB ribosomal operon P1 promoter.

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Selected References

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