Abstract
The tetracycline-dependent expression system has gained increasing popularity for the expression of any gene of interest. Careful choice of the expression vector has been suggested to exploit the full potential of this system. A novel tetracycline-sensitive expression vector based on a modified mouse mammary tumor virus promoter achieved considerably improved regulatory properties in a series of cell lines tested under transient and stable conditions. Therefore, the applicability of the tetracycline-dependent expression system can be largely enhanced by careful adaptation of the expression vector to the host cell line.
Full Text
The Full Text of this article is available as a PDF (39.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gossen M., Bonin A. L., Bujard H. Control of gene activity in higher eukaryotic cells by prokaryotic regulatory elements. Trends Biochem Sci. 1993 Dec;18(12):471–475. doi: 10.1016/0968-0004(93)90009-c. [DOI] [PubMed] [Google Scholar]
- Gossen M., Bujard H. Efficacy of tetracycline-controlled gene expression is influenced by cell type: commentary. Biotechniques. 1995 Aug;19(2):213–217. [PubMed] [Google Scholar]
- Gossen M., Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547–5551. doi: 10.1073/pnas.89.12.5547. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spengler D., Waeber C., Pantaloni C., Holsboer F., Bockaert J., Seeburg P. H., Journot L. Differential signal transduction by five splice variants of the PACAP receptor. Nature. 1993 Sep 9;365(6442):170–175. doi: 10.1038/365170a0. [DOI] [PubMed] [Google Scholar]