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. 1997 Mar 15;25(6):1313–1314. doi: 10.1093/nar/25.6.1313

Colorimetric detection of immobilised PCR products generated on a solid support.

A K Lockley 1, C G Jones 1, J S Bruce 1, S J Franklin 1, R G Bardsley 1
PMCID: PMC146568  PMID: 9092648

Abstract

By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associated with porcine malignant hyperthermia.

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Selected References

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  1. Fujii J., Otsu K., Zorzato F., de Leon S., Khanna V. K., Weiler J. E., O'Brien P. J., MacLennan D. H. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science. 1991 Jul 26;253(5018):448–451. doi: 10.1126/science.1862346. [DOI] [PubMed] [Google Scholar]
  2. Holloway B., Erdman D. D., Durigon E. L., Murtagh J. J., Jr An exonuclease-amplification coupled capture technique improves detection of PCR product. Nucleic Acids Res. 1993 Aug 11;21(16):3905–3906. doi: 10.1093/nar/21.16.3905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hornes E., Wasteson Y., Olsvik O. Detection of Escherichia coli heat-stable enterotoxin genes in pig stool specimens by an immobilized, colorimetric, nested polymerase chain reaction. J Clin Microbiol. 1991 Nov;29(11):2375–2379. doi: 10.1128/jcm.29.11.2375-2379.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. O'Brien P. J., Shen H., Cory C. R., Zhang X. Use of a DNA-based test for the mutation associated with porcine stress syndrome (malignant hyperthermia) in 10,000 breeding swine. J Am Vet Med Assoc. 1993 Sep 15;203(6):842–851. [PubMed] [Google Scholar]
  5. Rust S., Funke H., Assmann G. Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection. Nucleic Acids Res. 1993 Aug 11;21(16):3623–3629. doi: 10.1093/nar/21.16.3623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  7. Saiki R. K., Walsh P. S., Levenson C. H., Erlich H. A. Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6230–6234. doi: 10.1073/pnas.86.16.6230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Triglia T., Argyropoulos V. P., Davidson B. E., Kemp D. J. Colourimetric detection of PCR products using the DNA-binding protein TyrR. Nucleic Acids Res. 1990 Feb 25;18(4):1080–1080. doi: 10.1093/nar/18.4.1080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ugozzoli L., Wahlqvist J. M., Ehsani A., Kaplan B. E., Wallace R. B. Detection of specific alleles by using allele-specific primer extension followed by capture on solid support. Genet Anal Tech Appl. 1992 Aug;9(4):107–112. doi: 10.1016/1050-3862(92)90049-b. [DOI] [PubMed] [Google Scholar]
  10. Zhang Y., Coyne M. Y., Will S. G., Levenson C. H., Kawasaki E. S. Single-base mutational analysis of cancer and genetic diseases using membrane bound modified oligonucleotides. Nucleic Acids Res. 1991 Jul 25;19(14):3929–3933. doi: 10.1093/nar/19.14.3929. [DOI] [PMC free article] [PubMed] [Google Scholar]

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