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. 1997 Mar 15;25(6):1317–1318. doi: 10.1093/nar/25.6.1317

B lymphocyte-specific, Cre-mediated mutagenesis in mice.

R C Rickert 1, J Roes 1, K Rajewsky 1
PMCID: PMC146582  PMID: 9092650

Abstract

Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.

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Selected References

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