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. 1997 Jan;190(Pt 1):85–92. doi: 10.1046/j.1469-7580.1997.19010085.x

Stromal cell organisation in the mouse lymph node. A light and electron microscopic investigation using the zinc iodide-osmium technique

ENRICO CRIVELLATO 1,✉, FRANCO MALLARDI 1
PMCID: PMC1467586  PMID: 9034884

Abstract

The organisation of the stromal cell compartment in the mouse lymph node was studied by light and electron microscopy after tissue impregnation by the zinc iodide-osmium (ZIO) method. Fibroblastic reticular cells (FRCs) represented the main stromal cell population. These cells were located both in the cortical region and in the medulla and exhibited various configurations. In the cortex, FRCs were fusiform in shape and came into close proximity with the floor of the subcapsular sinus. In the medulla, the FRCs were shaped like irregular dendritic cells which formed a complex 3-dimensional network. The FRCs surrounded vascular structures such as capillaries and/or high endothelial venules; in these instances they were organised in a discontinuous sheath-like fashion around the vessel wall. By light and electron microscopy, FRCs have been observed to come in close spatial relationship with a number of cells in the lymph node, including sinus endothelial cells, the endothelium of high endothelial venules and capillaries, various types of lymphocytes, follicular dendritic cells and interdigitating cells. These microanatomical features are consistent with the proposal that FRCs may be involved in the communicative networks between the different lymph node compartments. In particular, the FRCs may be involved in the transport of molecules from the sinus compartment to the high endothelial venules or to the distinct cell populations in the lymphoid parenchyma.

Keywords: Fibroblastic reticular cells, osmium-zinc iodide stain

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Selected References

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  1. Anderson A. O., Anderson N. D. Lymphocyte emigration from high endothelial venules in rat lymph nodes. Immunology. 1976 Nov;31(5):731–748. [PMC free article] [PubMed] [Google Scholar]
  2. Anderson A. O., Anderson N. D. Studies on the structure and permeability of the microvasculature in normal rat lymph nodes. Am J Pathol. 1975 Sep;80(3):387–418. [PMC free article] [PubMed] [Google Scholar]
  3. Anderson A. O., Shaw S. T cell adhesion to endothelium: the FRC conduit system and other anatomic and molecular features which facilitate the adhesion cascade in lymph node. Semin Immunol. 1993 Aug;5(4):271–282. doi: 10.1006/smim.1993.1031. [DOI] [PubMed] [Google Scholar]
  4. BAIRATI A., AMANTE L., DEPETRIS S., PERNIS B. STUDIES ON THE ULTRASTRUCTURE OF THE LYMPH NODES. I. THE RETICULAR NETWORK. Z Zellforsch Mikrosk Anat. 1964 Aug 18;63:644–672. doi: 10.1007/BF00339912. [DOI] [PubMed] [Google Scholar]
  5. CLARK S. L., Jr The reticulum of lymph nodes in mice studied with the electron microscope. Am J Anat. 1962 May;110:217–257. doi: 10.1002/aja.1001100303. [DOI] [PubMed] [Google Scholar]
  6. Compton C. C., Raviola E. Structure of the sinus-lining cells in the popliteal lymph node of the rabbit. Anat Rec. 1985 Aug;212(4):408–423. doi: 10.1002/ar.1092120412. [DOI] [PubMed] [Google Scholar]
  7. Crocker J., Hopkins M. Histiocytic and dendritic reticulum cells shown by a zinc iodide-osmium technique. J Clin Pathol. 1984 Jun;37(6):620–627. doi: 10.1136/jcp.37.6.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dağdeviren A., Alp H., Ors U. New applications for the zinc iodide-osmium tetroxide technique. J Anat. 1994 Feb;184(Pt 1):83–91. [PMC free article] [PubMed] [Google Scholar]
  9. Degrassi A., Hilbert D. M., Rudikoff S., Anderson A. O., Potter M., Coon H. G. In vitro culture of primary plasmacytomas requires stromal cell feeder layers. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):2060–2064. doi: 10.1073/pnas.90.5.2060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Farr A. G., Berry M. L., Kim A., Nelson A. J., Welch M. P., Aruffo A. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues. J Exp Med. 1992 Nov 1;176(5):1477–1482. doi: 10.1084/jem.176.5.1477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fossum S., Vaaland J. L. The architecture of rat lymph nodes. I. Combined light and electron microscopy of lymph node cell types. Anat Embryol (Berl) 1983;167(2):229–246. doi: 10.1007/BF00298513. [DOI] [PubMed] [Google Scholar]
  12. Hanaoka K., Fujita N., Lee S. H., Seimiya H., Naito M., Tsuruo T. Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cells. Cancer Res. 1995 May 15;55(10):2186–2190. [PubMed] [Google Scholar]
  13. Kataoka S., Naito M., Fujita N., Ishii H., Ishii S., Yamori T., Nakajima M., Tsuruo T. Control of apoptosis and growth of malignant T lymphoma cells by lymph node stromal cells. Exp Cell Res. 1993 Aug;207(2):271–276. doi: 10.1006/excr.1993.1193. [DOI] [PubMed] [Google Scholar]
  14. MAILLET M. LE R'EACTIF AU TETRAOXYDE D'OSMIUM-IODURE DU ZINC. Z Mikrosk Anat Forsch. 1963;70:397–425. [PubMed] [Google Scholar]
  15. Markgraf R., von Gaudecker B., Müller-Hermelink H. K. The development of the human lymph node. Cell Tissue Res. 1982;225(2):387–413. doi: 10.1007/BF00214691. [DOI] [PubMed] [Google Scholar]
  16. Tykocinski M., Schinella R. A., Greco M. A. Fibroblastic reticulum cells in human lymph nodes. An ultrastructural study. Arch Pathol Lab Med. 1983 Aug;107(8):418–422. [PubMed] [Google Scholar]

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