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. 1997 Apr;190(Pt 3):377–395. doi: 10.1046/j.1469-7580.1997.19030377.x

Spermiogenesis and spermiation in a marsupial, the tammar wallaby (Macropus eugenii)

MINJIE LIN 1, AMANDA HARMAN 1, JOHN C RODGER 1
PMCID: PMC1467618  PMID: 9147224

Abstract

Fourteen steps of spermatid development in the tammar wallaby (Macropus eugenii), from the newly formed spermatid to the release of the spermatozoon into the lumen of the seminiferous tubules, were recognised at the ultrastructural level using transmission and scanning electron microscopy. This study confirmed that although the main events are generally similar, the process of the differentiation of the spermatid in marsupials is notably different and relatively more complex than that in most studied eutherian mammals and birds. For example, the sperm head rotated twice in the late stage of spermiogenesis: the shape of the spermatid changed from a T-shape at step 10 into a streamlined shape in step 14, and then back to T-shape in the testicular spermatozoa. Some unique figures occurring during the spermiogenesis in other marsupial species, such as the presence of Sertoli cell spurs, the nuclear ring and the subacrosomal space, were also found in the tammar wallaby. However, an important new finding of this study was the development of the postacrosome complex (PAC), a special structure that was first evident as a line of electron dense material on the nuclear membrane of the step 7 spermatid. Subsequently it became a discontinuous line of electron particles, and migrated from the ventral side of the nucleus to the area just behind the posterior end of the acrosome, which was closely located to the sperm–egg fusion site proposed for Monodelphis domestica (Taggart et al. 1993). The PAC and its possible role in both American and Australian marsupials requires detailed examination. Distinct immature features were discovered in the wallaby testicular spermatozoa. A scoop shape of the acrosome was found on the testicular spermatozoa of the tammar wallaby, which was completely different to the compact button shape of acrosome in ejaculated spermatozoa. The fibre network found beneath the cytoplasm membrane of the midpiece of the ejaculated sperm also did not occur in the testicular spermatozoa, although the structure of the principal piece was fully formed and had no obvious morphological difference from that of the epididymal and ejaculated spermatozoa. The time frame of the formation of morphologically mature spermatozoa in the epididymis of the tammar wallaby needs to be determined by further studies.

Keywords: Marsupial spermiogenesis, spermatogenesis, spermatids, testicular spermatozoa, acrosome, tammar wallaby

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Selected References

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  1. Bedford J. M., Moore H. D., Franklin L. E. Significance of the equatorial segment of the acrosome of the spermatozoon in eutherian mammals. Exp Cell Res. 1979 Mar 1;119(1):119–126. doi: 10.1016/0014-4827(79)90341-0. [DOI] [PubMed] [Google Scholar]
  2. CLELAND K. W. Acrosome formation in bandicoot spermiogenesis. Nature. 1956 Feb 25;177(4504):387–388. doi: 10.1038/177387a0. [DOI] [PubMed] [Google Scholar]
  3. CLELAND K. W., ROTHSCHILD L. The bandicoot spermatozoon: an electron microscope study of the tail. Proc R Soc Lond B Biol Sci. 1959 Jan 27;150(938):24–42. doi: 10.1098/rspb.1959.0005. [DOI] [PubMed] [Google Scholar]
  4. Fawcett D. W., Anderson W. A., Phillips D. M. Morphogenetic factors influencing the shape of the sperm head. Dev Biol. 1971 Oct;26(2):220–251. doi: 10.1016/0012-1606(71)90124-2. [DOI] [PubMed] [Google Scholar]
  5. Gunawardana V. K., Scott M. G. Ultrastructural studies on the differentiation of spermatids in the domestic fowl. J Anat. 1977 Dec;124(Pt 3):741–755. [PMC free article] [PubMed] [Google Scholar]
  6. Harding H. R., Carrick F. N., Shorey C. D. Spermiogenesis in the brush-tailed possum, Trichosurus vulpecula (Marsupialia). The development of the acrosome. Cell Tissue Res. 1976 Aug 16;171(1):75–90. doi: 10.1007/BF00219701. [DOI] [PubMed] [Google Scholar]
  7. Jones R. C., Lin M. Spermatogenesis in birds. Oxf Rev Reprod Biol. 1993;15:233–264. [PubMed] [Google Scholar]
  8. Lin M., Jones R. C. Spermiogenesis and spermiation in the Japanese quail (Coturnix coturnix japonica). J Anat. 1993 Dec;183(Pt 3):525–535. [PMC free article] [PubMed] [Google Scholar]
  9. Lin M., Thorne M. H., Martin I. C., Sheldon B. L., Jones R. C. Electron microscopy of the seminiferous epithelium in the triploid (ZZZ and ZZW) fowl, Gallus domesticus. J Anat. 1995 Jun;186(Pt 3):563–576. [PMC free article] [PubMed] [Google Scholar]
  10. Moore H. D., Bedford J. M. Ultrastructure of the equatorial segment of hamster spermatozoa during penetration of oocytes. J Ultrastruct Res. 1978 Feb;62(2):110–117. doi: 10.1016/s0022-5320(78)90013-8. [DOI] [PubMed] [Google Scholar]
  11. NAGANO T. Observations on the fine structure of the developing spermatid in the domestic chicken. J Cell Biol. 1962 Aug;14:193–205. doi: 10.1083/jcb.14.2.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okamura F., Nishiyama H. Penetration of spermatozoon into the ovum and transformation of the sperm nucleus into the male pronucleus in the domestic fowl, Gallus gallus. Cell Tissue Res. 1978 Jun 26;190(1):89–98. doi: 10.1007/BF00210039. [DOI] [PubMed] [Google Scholar]
  13. Okamura F., Nishiyama H. The early development of the tail and the transformation of the shape of the nucleus of the spermatid of the domestic fowl, Gallus gallus. Cell Tissue Res. 1976 Jun 28;169(3):345–359. doi: 10.1007/BF00219607. [DOI] [PubMed] [Google Scholar]
  14. Phillips D. M. Development of spermatozoa in the wooly opossum with special reference to the shaping of the sperm head. J Ultrastruct Res. 1970 Nov;33(3):369–380. doi: 10.1016/s0022-5320(70)90029-8. [DOI] [PubMed] [Google Scholar]
  15. Picheral B., Charbonneau M. Anuran fertilization: a morphological reinvestigation of some early events. J Ultrastruct Res. 1982 Dec;81(3):306–321. doi: 10.1016/s0022-5320(82)90059-4. [DOI] [PubMed] [Google Scholar]
  16. Rodger J. C., Bedford J. M. Separation of sperm pairs and sperm-egg interaction in the opossum, Didelphis virginiana. J Reprod Fertil. 1982 Jan;64(1):171–179. doi: 10.1530/jrf.0.0640171. [DOI] [PubMed] [Google Scholar]
  17. Russell L., Clermont Y. Anchoring device between Sertoli cells and late spermatids in rat seminiferous tubules. Anat Rec. 1976 Jul;185(3):259–278. doi: 10.1002/ar.1091850302. [DOI] [PubMed] [Google Scholar]
  18. Setiadi D., Lin M., Rodger J. C. Posttesticular development of spermatozoa of the tammar wallaby (Macropus eugenii). J Anat. 1997 Feb;190(Pt 2):275–288. doi: 10.1046/j.1469-7580.1997.19020275.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Taggart D. A., O'Brien H. P., Moore H. D. Ultrastructural characteristics of in vivo and in vitro fertilization in the grey short-tailed opossum, Monodelphis domestica. Anat Rec. 1993 Sep;237(1):21–37. doi: 10.1002/ar.1092370104. [DOI] [PubMed] [Google Scholar]
  20. Temple-Smith P. D., Bedford J. M. The features of sperm maturation in the epididymis of a marsupial, the brushtailed possum Trichosurus vulpecula. Am J Anat. 1976 Dec;147(4):471–499. doi: 10.1002/aja.1001470407. [DOI] [PubMed] [Google Scholar]

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