Skip to main content
PMC home page
Journal of Anatomy logoLink to Journal of Anatomy
. 1997 Jul;191(Pt 1):57–64. doi: 10.1046/j.1469-7580.1997.19110057.x

Analysis of fused maxillary incisor dentition in p53-deficient exencephalic mice

M H KAUFMAN 1 , D B KAUFMAN 1 , R M BRUNE 1 , M STARK 3 , J F ARMSTRONG 2 , A R CLARKE 2
PMCID: PMC1467659  PMID: 9279659

Abstract

Out of a total of 21 exencephalic p53-deficient embryonic and newborn mice, 6 (28.6%) possessed fused maxillary incisor teeth. On histological analysis of the 5 examples seen on day 19.5 of gestation and newborn mice, 3 varieties were observed: an example of ‘simple’ fusion, 3 examples of simple fusion each of which contained a ‘dens in dente’ (‘tooth within a tooth’), and a single example in which the fused teeth were associated with a median supernumerary incisor tooth which, while deeply indenting the labial surface of the fused teeth, was in all locations a completely separate unit. 3-D reconstructions of the fused teeth demonstrated that they were all of the fusio subtotalis variety. No gross abnormalities were observed in the other dentition in these mice. It is noted that in mice fused maxillary incisor teeth are relatively commonly associated with both hypervitaminosis A-induced and trypan blue-induced exencephaly. It is believed that the presence of dens in dente within fused maxillary incisor teeth has only once been reported in mice, and the association between fused maxillary incisor teeth and a median supernumerary incisor tooth has not previously been reported in this species.

Keywords: Exencephaly, fused maxillary incisor teeth, supernumerary incisor tooth, 3-D reconstruction

Full Text

The Full Text of this article is available as a PDF (621.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. F., Kaufman M. H., Harrison D. J., Clarke A. R. High-frequency developmental abnormalities in p53-deficient mice. Curr Biol. 1995 Aug 1;5(8):931–936. doi: 10.1016/s0960-9822(95)00183-7. [DOI] [PubMed] [Google Scholar]
  2. Atkins C. O., Jr, Mourino A. P. Management of a supernumerary tooth fused to a permanent maxillary central incisor. Oral Surg Oral Med Oral Pathol. 1986 Feb;61(2):146–148. doi: 10.1016/0030-4220(86)90176-3. [DOI] [PubMed] [Google Scholar]
  3. Augsburger R. A., Brandebura J., Jr Bilateral dens invaginatus with associated radicular cysts. A case report. Oral Surg Oral Med Oral Pathol. 1978 Aug;46(2):260–264. doi: 10.1016/0030-4220(78)90201-3. [DOI] [PubMed] [Google Scholar]
  4. BOYD J. D., MILES A. E. W. An erupted tooth in a cyclops foetus. Br Dent J. 1951 Oct 2;91(7):173–181. [PubMed] [Google Scholar]
  5. Baldock R., Bard J., Kaufman M., Davidson D. A real mouse for your computer. Bioessays. 1992 Jul;14(7):501–502. doi: 10.1002/bies.950140713. [DOI] [PubMed] [Google Scholar]
  6. Bazan M. T. Fusion of maxillary incisors across the midline: clinical report. Pediatr Dent. 1983 Sep;5(3):220–221. [PubMed] [Google Scholar]
  7. Beynon A. D. Developing dens invaginatus (dens in dente). A quantitative microradiographic study and a reconsideration of the histogenesis of this condition. Br Dent J. 1982 Oct 5;153(7):255–260. doi: 10.1038/sj.bdj.4804912. [DOI] [PubMed] [Google Scholar]
  8. Blank B. S., Ogg R. R., Levy A. R. A fused central incisor. Periodontal considerations in comprehensive treatment. J Periodontol. 1985 Jan;56(1):21–24. doi: 10.1902/jop.1985.56.1.21. [DOI] [PubMed] [Google Scholar]
  9. Brook A. H., Winter G. B. Double teeth. A retrospective study of 'geminated' and 'fused' teeth in children. Br Dent J. 1970 Aug 4;129(3):123–130. doi: 10.1038/sj.bdj.4802533. [DOI] [PubMed] [Google Scholar]
  10. Buenviaje T. M., Rapp R. Dental anomalies in children: a clinical and radiographic survey. ASDC J Dent Child. 1984 Jan-Feb;51(1):42–46. [PubMed] [Google Scholar]
  11. Burton D. J., Saffos R. O., Scheffer R. B. Multiple bilateral dens in dente as a factor in the etiology of multiple periapical lesions. Oral Surg Oral Med Oral Pathol. 1980 Jun;49(6):496–499. doi: 10.1016/0030-4220(80)90069-9. [DOI] [PubMed] [Google Scholar]
  12. Clarke A. R., Maandag E. R., van Roon M., van der Lugt N. M., van der Valk M., Hooper M. L., Berns A., te Riele H. Requirement for a functional Rb-1 gene in murine development. Nature. 1992 Sep 24;359(6393):328–330. doi: 10.1038/359328a0. [DOI] [PubMed] [Google Scholar]
  13. Clarke A. R., Purdie C. A., Harrison D. J., Morris R. G., Bird C. C., Hooper M. L., Wyllie A. H. Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature. 1993 Apr 29;362(6423):849–852. doi: 10.1038/362849a0. [DOI] [PubMed] [Google Scholar]
  14. Curzon J. A., Curzon M. E. Congenital dental anomalies in a group of British Columbia children. J Can Dent Assoc (Tor) 1967 Oct;33(10):554–558. [PubMed] [Google Scholar]
  15. Donehower L. A., Harvey M., Slagle B. L., McArthur M. J., Montgomery C. A., Jr, Butel J. S., Bradley A. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. Nature. 1992 Mar 19;356(6366):215–221. doi: 10.1038/356215a0. [DOI] [PubMed] [Google Scholar]
  16. FORRESTER R. M., MILLER J. The dental changes associated with kernikterus. Arch Dis Child. 1955 Jun;30(151):224–231. doi: 10.1136/adc.30.151.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jacks T., Remington L., Williams B. O., Schmitt E. M., Halachmi S., Bronson R. T., Weinberg R. A. Tumor spectrum analysis in p53-mutant mice. Curr Biol. 1994 Jan 1;4(1):1–7. doi: 10.1016/s0960-9822(00)00002-6. [DOI] [PubMed] [Google Scholar]
  18. KALTER H., WARKANY J. Experimental production of congenital malformations in strains of inbred mice by maternal treatment with hypervitaminosis A. Am J Pathol. 1961 Jan;38:1–21. [PMC free article] [PubMed] [Google Scholar]
  19. KNUDSEN P. A. CONGENITAL MALFORMATIONS OF UPPER INCISORS IN EXENCEPHALIC MOUSE EMBRYOS, INDUCED BY HYPERVITAMINOSIS A. I. TYPES AND FREQUENCY. Acta Odontol Scand. 1965 Feb;23:71–89. doi: 10.3109/00016356509044310. [DOI] [PubMed] [Google Scholar]
  20. Knudsen P. A. Congenitalmalformations of upper incisors in exencephalic mouse embryos, induced by hypervitaminosis A. II. Morphology of fused upper incisors. Acta Odontol Scand. 1965 Aug;23(4):391–409. doi: 10.3109/00016356509007521. [DOI] [PubMed] [Google Scholar]
  21. Knudsen P. A. Malformations of upper incisors in mouse embryos with exencephaly, induced by trypan blue. Acta Odontol Scand. 1966 Dec;24(6):647–675. doi: 10.3109/00016356609028734. [DOI] [PubMed] [Google Scholar]
  22. LEVITAS T. C. GEMINATION, FUSION, TWINNING AND CONCRESCENCE. ASDC J Dent Child. 1965;32:93–100. [PubMed] [Google Scholar]
  23. Luongo C., Moser A. R., Gledhill S., Dove W. F. Loss of Apc+ in intestinal adenomas from Min mice. Cancer Res. 1994 Nov 15;54(22):5947–5952. [PubMed] [Google Scholar]
  24. MILES A. E. Malformations of the teeth. Proc R Soc Med. 1954 Oct;47(10):817–826. [PMC free article] [PubMed] [Google Scholar]
  25. NISWANDER J. D., SUJAKU C. CONGENITAL ANOMALIES OF TEETH IN JAPANESE CHILDREN. Am J Phys Anthropol. 1963 Dec;21:569–574. doi: 10.1002/ajpa.1330210413. [DOI] [PubMed] [Google Scholar]
  26. O'Reilly P. M. A structural and ultrastructural study of a fused tooth. J Endod. 1989 Sep;15(9):442–446. doi: 10.1016/S0099-2399(89)80180-3. [DOI] [PubMed] [Google Scholar]
  27. Purdie C. A., Harrison D. J., Peter A., Dobbie L., White S., Howie S. E., Salter D. M., Bird C. C., Wyllie A. H., Hooper M. L. Tumour incidence, spectrum and ploidy in mice with a large deletion in the p53 gene. Oncogene. 1994 Feb;9(2):603–609. [PubMed] [Google Scholar]
  28. Ringwald M., Baldock R., Bard J., Kaufman M., Eppig J. T., Richardson J. E., Nadeau J. H., Davidson D. A database for mouse development. Science. 1994 Sep 30;265(5181):2033–2034. doi: 10.1126/science.8091224. [DOI] [PubMed] [Google Scholar]
  29. Robins M. W., Rowlatt C. Dental abnormalities in aged mice. Gerontologia. 1971;17(5):261–272. doi: 10.1159/000211833. [DOI] [PubMed] [Google Scholar]
  30. Sah V. P., Attardi L. D., Mulligan G. J., Williams B. O., Bronson R. T., Jacks T. A subset of p53-deficient embryos exhibit exencephaly. Nat Genet. 1995 Jun;10(2):175–180. doi: 10.1038/ng0695-175. [DOI] [PubMed] [Google Scholar]
  31. Su L. K., Kinzler K. W., Vogelstein B., Preisinger A. C., Moser A. R., Luongo C., Gould K. A., Dove W. F. Multiple intestinal neoplasia caused by a mutation in the murine homolog of the APC gene. Science. 1992 May 1;256(5057):668–670. doi: 10.1126/science.1350108. [DOI] [PubMed] [Google Scholar]
  32. VIA W. F., Jr, CHURCHILL J. A. Relationships of cerebral disorder to faults in dental enamel. AMA J Dis Child. 1957 Aug;94(2):137–142. doi: 10.1001/archpedi.1957.04030030031006. [DOI] [PubMed] [Google Scholar]
  33. Vajrabhaya L. Nonsurgical endodontic treatment of a tooth with double dens in dente. J Endod. 1989 Jul;15(7):323–325. doi: 10.1016/s0099-2399(89)80056-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Anatomy are provided here courtesy of Anatomical Society of Great Britain and Ireland

RESOURCES