Skip to main content
PMC home page
Journal of Anatomy logoLink to Journal of Anatomy
. 1997 Nov;191(Pt 4):561–570. doi: 10.1046/j.1469-7580.1997.19140561.x

Immunohistochemistry of collagen types II and X, and enzyme-histochemistry of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible

SHUNICHI SHIBATA 1 ,✉, KENJI FUKADA 2 , SHOICHI SUZUKI 2 , YASUO YAMASHITA 1
PMCID: PMC1467723  PMID: 9449075

Abstract

We investigated the immunohistochemical localisation of types II and X collagen as well as the cytochemical localisation of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible on d 14–16 of pregnancy. On d 14 of pregnancy, although no immunostaining for types II and X collagen was observed, alkaline phosphatase activity was detected in all cells in the anlage of the future condylar process. On d 15 of pregnancy, immunostaining for both collagen types was simultaneously detected in the primarily formed condylar cartilage. Alkaline phosphatase activity was also detected in chondrocytes at this stage. By d 16 of pregnancy, the hypertrophic cell zone rapidly increased in size. These findings strongly support a periosteal origin for the condylar cartilage of the fetal mouse mandible, and show that progenitor cells for condylar cartilage rapidly or directly differentiate into hypertrophic chondrocytes.

Keywords: Bone, periosteum, chondrocytes

Full Text

The Full Text of this article is available as a PDF (1.5 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURSTONE M. S. Histochemical observations on enzymatic processes in bones and teeth. Ann N Y Acad Sci. 1960 Mar 29;85:431–444. doi: 10.1111/j.1749-6632.1960.tb49972.x. [DOI] [PubMed] [Google Scholar]
  2. Doty S. B., Schofield B. H. Enzyme histochemistry of bone and cartilage cells. Prog Histochem Cytochem. 1976;8(1):1–38. doi: 10.1016/s0079-6336(76)80010-1. [DOI] [PubMed] [Google Scholar]
  3. Fischer G. Die Verteilung und Aktivität von Enzymen in der Humerusepiphyse von Albinoratten bestimmten Alters. Acta Anat (Basel) 1980;106(1):150–157. [PubMed] [Google Scholar]
  4. Gibson G. J., Flint M. H. Type X collagen synthesis by chick sternal cartilage and its relationship to endochondral development. J Cell Biol. 1985 Jul;101(1):277–284. doi: 10.1083/jcb.101.1.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Iyama K., Ninomiya Y., Olsen B. R., Linsenmayer T. F., Trelstad R. L., Hayashi M. Spatiotemporal pattern of type X collagen gene expression and collagen deposition in embryonic chick vertebrae undergoing endochondral ossification. Anat Rec. 1991 Apr;229(4):462–472. doi: 10.1002/ar.1092290405. [DOI] [PubMed] [Google Scholar]
  6. Luder H. U., Leblond C. P., von der Mark K. Cellular stages in cartilage formation as revealed by morphometry, radioautography and type II collagen immunostaining of the mandibular condyle from weanling rats. Am J Anat. 1988 Jul;182(3):197–214. doi: 10.1002/aja.1001820302. [DOI] [PubMed] [Google Scholar]
  7. Miyake T., Cameron A. M., Hall B. K. Stage-specific expression patterns of alkaline phosphatase during development of the first arch skeleton in inbred C57BL/6 mouse embryos. J Anat. 1997 Feb;190(Pt 2):239–260. doi: 10.1046/j.1469-7580.1997.19020239.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mizoguchi I., Nakamura M., Takahashi I., Kagayama M., Mitani H. An immunohistochemical study of localization of type I and type II collagens in mandibular condylar cartilage compared with tibial growth plate. Histochemistry. 1990;93(6):593–599. doi: 10.1007/BF00272201. [DOI] [PubMed] [Google Scholar]
  9. Mizoguchi I., Takahashi I., Nakamura M., Sasano Y., Sato S., Kagayama M., Mitani H. An immunohistochemical study of regional differences in the distribution of type I and type II collagens in rat mandibular condylar cartilage. Arch Oral Biol. 1996 Aug-Sep;41(8-9):863–869. doi: 10.1016/s0003-9969(96)00021-0. [DOI] [PubMed] [Google Scholar]
  10. Mizoguchi I., Takahashi I., Sasano Y., Kagayama M., Mitani H. Localization of types I, II and III collagen and glycosaminoglycans in the mandibular condyle of growing monkeys: an immunohistochemical study. Anat Embryol (Berl) 1997 Feb;195(2):127–135. doi: 10.1007/s004290050031. [DOI] [PubMed] [Google Scholar]
  11. Pirttiniemi P., Kantomaa T., Salo L., Tuominen M. Effect of reduced articular function on deposition of type I and type II collagens in the mandibular condylar cartilage of the rat. Arch Oral Biol. 1996 Jan;41(1):127–131. doi: 10.1016/0003-9969(95)00102-6. [DOI] [PubMed] [Google Scholar]
  12. Poole A. R., Pidoux I. Immunoelectron microscopic studies of type X collagen in endochondral ossification. J Cell Biol. 1989 Nov;109(5):2547–2554. doi: 10.1083/jcb.109.5.2547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Salo L. A., Hoyland J., Ayad S., Kielty C. M., Freemont A., Pirttiniemi P., Kantomaa T., Grant M. E., Thomas J. T. The expression of types X and VI collagen and fibrillin in rat mandibular condylar cartilage. Response to mastication forces. Acta Odontol Scand. 1996 Oct;54(5):295–302. doi: 10.3109/00016359609003541. [DOI] [PubMed] [Google Scholar]
  14. Salo L., Kantomaa T. Type II collagen expression in the mandibular condyle during growth adaptation: an experimental study in the rabbit. Calcif Tissue Int. 1993 Jun;52(6):465–469. doi: 10.1007/BF00571338. [DOI] [PubMed] [Google Scholar]
  15. Schmid T. M., Linsenmayer T. F. Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues. J Cell Biol. 1985 Feb;100(2):598–605. doi: 10.1083/jcb.100.2.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shibata S., Suzuki S., Tengan T., Ishii M., Kuroda T. A histological study of the developing condylar cartilage of the fetal mouse mandible using coronal sections. Arch Oral Biol. 1996 Jan;41(1):47–54. doi: 10.1016/0003-9969(95)00105-0. [DOI] [PubMed] [Google Scholar]
  17. Silbermann M., Frommer J. Phosphatases within the cartilage of the mandibular condyle of the mouse. J Anat. 1973 Dec;116(Pt 3):335–345. [PMC free article] [PubMed] [Google Scholar]
  18. Silbermann M., Reddi A. H., Hand A. R., Leapman R. D., Von der Mark K., Franzen A. Further characterisation of the extracellular matrix in the mandibular condyle in neonatal mice. J Anat. 1987 Apr;151:169–188. [PMC free article] [PubMed] [Google Scholar]
  19. Silbermann M., von der Mark K. An immunohistochemical study of the distribution of matrical proteins in the mandibular condyle of neonatal mice. I. Collagens. J Anat. 1990 Jun;170:11–22. [PMC free article] [PubMed] [Google Scholar]
  20. Takahashi I., Mizoguchi I., Nakamura M., Kagayama M., Mitani H. Effects of lateral pterygoid muscle hyperactivity on differentiation of mandibular condyles in rats. Anat Rec. 1995 Mar;241(3):328–336. doi: 10.1002/ar.1092410306. [DOI] [PubMed] [Google Scholar]
  21. Ustünel I., Demir R. A histochemical study on the enzymatic activity in the proximal epiphysis of the humerus during the prenatal and postnatal periods in rats. Ann Anat. 1995 Jan;177(1):73–83. doi: 10.1016/S0940-9602(11)80134-2. [DOI] [PubMed] [Google Scholar]
  22. Vilmann A., Vilmann H. Os penis of the rat. IV. The proximal growth cartilage. Acta Anat (Basel) 1983;117(2):136–144. doi: 10.1159/000145779. [DOI] [PubMed] [Google Scholar]
  23. Vinkka-Puhakka H., Thesleff I. Initiation of secondary cartilage in the mandible of the Syrian hamster in the absence of muscle function. Arch Oral Biol. 1993 Jan;38(1):49–54. doi: 10.1016/0003-9969(93)90154-e. [DOI] [PubMed] [Google Scholar]
  24. von der Mark K., von der Mark H., Gay S. Study of differential collagen synthesis during development of the chick embryo by immunofluroescence. II. Localization of type I and type II collagen during long bone development. Dev Biol. 1976 Oct 15;53(2):153–170. doi: 10.1016/0012-1606(76)90220-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Anatomy are provided here courtesy of Anatomical Society of Great Britain and Ireland

RESOURCES