Skip to main content
Journal of Anatomy logoLink to Journal of Anatomy
. 1998 Feb;192(Pt 2):203–210. doi: 10.1046/j.1469-7580.1998.19220203.x

Muscle fibre types and their distribution in the biceps and triceps brachii of the rat and rabbit

I FUENTES 1, A R COBOS 1, L A G SEGADE 1,
PMCID: PMC1467754  PMID: 9643421

Abstract

Muscle fibre type composition and distribution in the biceps brachii (long head) and triceps brachii (long head) of the rat and rabbit were investigated using the following histochemical techniques: myosin ATPase, with preincubation at pH 10.4 and 4.35; succinate dehydrogenase (SDH) and glycogen phosphorylase. The muscle fibres were classified into slow-twitch (SO), fast-twitch glycolytic (FG), fast-twitch oxidative glycolytic (FOG and FOg) and fast-twitch oxidative fibres (FO). Significant differences in the regional distribution of muscle fibre types have been observed between the rat and the rabbit. In the rat, SO fibres were restricted to the deep regions of both biceps and triceps brachii, whereas FG fibres were located in the intermediate and superficial regions (the superficial regions contained the highest percentages of FG fibres). In the rabbit, SO and FG fibres were spread over the entire muscle, although SO and FG fibres were most abundant in the deep and superficial regions respectively. These findings indicate that the biceps and triceps brachii are more regionalised in the rat than in the rabbit.

Keywords: Skeletal muscle, muscle histochemistry

Full Text

The Full Text of this article is available as a PDF (509.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acosta L., Jr, Roy R. R. Fiber-type composition of selected hindlimb muscles of a primate (cynomolgus monkey). Anat Rec. 1987 Jun;218(2):136–141. doi: 10.1002/ar.1092180207. [DOI] [PubMed] [Google Scholar]
  2. Alnaqeeb M. A., Goldspink G. Changes in fibre type, number and diameter in developing and ageing skeletal muscle. J Anat. 1987 Aug;153:31–45. [PMC free article] [PubMed] [Google Scholar]
  3. Armstrong R. B., Saubert C. W., 4th, Seeherman H. J., Taylor C. R. Distribution of fiber types in locomotory muscles of dogs. Am J Anat. 1982 Jan;163(1):87–98. doi: 10.1002/aja.1001630107. [DOI] [PubMed] [Google Scholar]
  4. Brooke M. H., Kaiser K. K. Muscle fiber types: how many and what kind? Arch Neurol. 1970 Oct;23(4):369–379. doi: 10.1001/archneur.1970.00480280083010. [DOI] [PubMed] [Google Scholar]
  5. Chanaud C. M., Pratt C. A., Loeb G. E. Functionally complex muscles of the cat hindlimb. V. The roles of histochemical fiber-type regionalization and mechanical heterogeneity in differential muscle activation. Exp Brain Res. 1991;85(2):300–313. doi: 10.1007/BF00229408. [DOI] [PubMed] [Google Scholar]
  6. Collatos T. C., Edgerton V. R., Smith J. L., Botterman B. R. Contractile properties and fiber type compositions of flexors and extensors of elbow joint in cat: implications for motor control. J Neurophysiol. 1977 Nov;40(6):1292–1300. doi: 10.1152/jn.1977.40.6.1292. [DOI] [PubMed] [Google Scholar]
  7. Dum R. P., Kennedy T. T. Physiological and histochemical characteristics of motor units in cat tibialis anterior and extensor digitorum longus muscles. J Neurophysiol. 1980 Jun;43(6):1615–1630. doi: 10.1152/jn.1980.43.6.1615. [DOI] [PubMed] [Google Scholar]
  8. ERANKO O., PALKAMA A. Improved localization of phosphorylase by the use of polyvinyl pyrrolidone and high substrate concentration. J Histochem Cytochem. 1961 Sep;9:585–585. doi: 10.1177/9.5.585. [DOI] [PubMed] [Google Scholar]
  9. Füchtbauer E. M., Rowlerson A. M., Götz K., Friedrich G., Mabuchi K., Gergely J., Jockusch H. Direct correlation of parvalbumin levels with myosin isoforms and succinate dehydrogenase activity on frozen sections of rodent muscle. J Histochem Cytochem. 1991 Mar;39(3):355–361. doi: 10.1177/39.3.1825216. [DOI] [PubMed] [Google Scholar]
  10. Gonyea W. J., Marushia S. A., Dixon J. A. Morphological organization and contractile properties of the wrist flexor muscles in the cat. Anat Rec. 1981 Mar;199(3):321–339. doi: 10.1002/ar.1091990303. [DOI] [PubMed] [Google Scholar]
  11. Gonyea W., Bonde-Petersen F. Contraction properties and fiber types of some forelimb and hind limb muscles in the cat. Exp Neurol. 1977 Nov;57(2):637–644. doi: 10.1016/0014-4886(77)90095-4. [DOI] [PubMed] [Google Scholar]
  12. Gunn H. M. Differences in the histochemical properties of skeletal muscles of different breeds of horses and dogs. J Anat. 1978 Dec;127(Pt 3):615–634. [PMC free article] [PubMed] [Google Scholar]
  13. Guth L., Samaha F. J. Procedure for the histochemical demonstration of actomyosin ATPase. Exp Neurol. 1970 Aug;28(2):365–367. [PubMed] [Google Scholar]
  14. Hermanson J. W., Hegemann-Monachelli M. T., Daaod M. J., LaFramboise W. A. Correlation of myosin isoforms with anatomical divisions in equine musculus biceps brachii. Acta Anat (Basel) 1991;141(4):369–376. doi: 10.1159/000147149. [DOI] [PubMed] [Google Scholar]
  15. Hermanson J. W., Hurley K. J. Architectural and histochemical analysis of the biceps brachii muscle of the horse. Acta Anat (Basel) 1990;137(2):146–156. doi: 10.1159/000146875. [DOI] [PubMed] [Google Scholar]
  16. Kugler P., Wrobel K. H. Meldola blue: a new electron carrier for the histochemical demonstration of dehydrogenases (SDH, LDH, G-6-PDH). Histochemistry. 1978 Dec 29;59(2):97–109. doi: 10.1007/BF00518505. [DOI] [PubMed] [Google Scholar]
  17. Latorre R., Gil F., Vázquez J. M., Moreno F., Mascarello F., Ramirez G. Skeletal muscle fibre types in the dog. J Anat. 1993 Jun;182(Pt 3):329–337. [PMC free article] [PubMed] [Google Scholar]
  18. Lexell J., Jarvis J. C., Currie J., Downham D. Y., Salmons S. Fibre type composition of rabbit tibialis anterior and extensor digitorum longus muscles. J Anat. 1994 Aug;185(Pt 1):95–101. [PMC free article] [PubMed] [Google Scholar]
  19. Lind A., Kernell D. Myofibrillar ATPase histochemistry of rat skeletal muscles: a "two-dimensional" quantitative approach. J Histochem Cytochem. 1991 May;39(5):589–597. doi: 10.1177/39.5.1826695. [DOI] [PubMed] [Google Scholar]
  20. Maltin C. A., Delday M. I., Baillie A. G., Grubb D. A., Garlick P. J. Fiber-type composition of nine rat muscles. I. Changes during the first year of life. Am J Physiol. 1989 Dec;257(6 Pt 1):E823–E827. doi: 10.1152/ajpendo.1989.257.6.E823. [DOI] [PubMed] [Google Scholar]
  21. Maxwell L. C., Barclay J. K., Mohrman D. E., Faulkner J. A. Physiological characteristics of skeletal muscles of dogs and cats. Am J Physiol. 1977 Jul;233(1):C14–C18. doi: 10.1152/ajpcell.1977.233.1.C14. [DOI] [PubMed] [Google Scholar]
  22. McIntosh J. S., Ringqvist M., Schmidt E. M. Fiber type composition of monkey forearm muscle. Anat Rec. 1985 Apr;211(4):403–409. doi: 10.1002/ar.1092110405. [DOI] [PubMed] [Google Scholar]
  23. Meijer A. E. Improved histochemical method for the demonstration of the activity of alpha-glucan phosphorylase. I. The use of glucosyl acceptor dextran. Histochemie. 1968;12(3):244–252. doi: 10.1007/BF00306002. [DOI] [PubMed] [Google Scholar]
  24. Nygaard E., Sanchez J. Intramuscular variation of fiber types in the brachial biceps and the lateral vastus muscles of elderly men: how representative is a small biopsy sample? Anat Rec. 1982 Aug;203(4):451–459. doi: 10.1002/ar.1092030404. [DOI] [PubMed] [Google Scholar]
  25. Peter J. B., Barnard R. J., Edgerton V. R., Gillespie C. A., Stempel K. E. Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochemistry. 1972 Jul 4;11(14):2627–2633. doi: 10.1021/bi00764a013. [DOI] [PubMed] [Google Scholar]
  26. Pullen A. H. The distribution and relative sizes of three histochemical fibre types in the rat tibialis anterior muscle. J Anat. 1977 Feb;123(Pt 1):1–19. [PMC free article] [PubMed] [Google Scholar]
  27. Shorey C. D., Cleland K. W. Morphometric analysis of frozen transverse sections of human skeletal muscle taken post-mortem. Acta Anat (Basel) 1988;131(1):30–34. doi: 10.1159/000146481. [DOI] [PubMed] [Google Scholar]
  28. Spurway N. C., Murray M. G., Gilmour W. H., Montgomery I. Quantitative skeletal muscle histochemistry of four east African ruminants. J Anat. 1996 Apr;188(Pt 2):455–472. [PMC free article] [PubMed] [Google Scholar]
  29. Spurway N. Interrelationship between myosin-based and metabolism-based classifications of skeletal muscle fibers. J Histochem Cytochem. 1981 Jan;29(1):87–90. doi: 10.1177/29.1.7204949. [DOI] [PubMed] [Google Scholar]
  30. Suzuki A. Composition of myofiber types in limb muscles of the house shrew (Suncus murinus): lack of type I myofibers. Anat Rec. 1990 Sep;228(1):23–30. doi: 10.1002/ar.1092280105. [DOI] [PubMed] [Google Scholar]
  31. Suzuki A., Tamate H. Distribution of myofiber types in the hip and thigh musculature of sheep. Anat Rec. 1988 May;221(1):494–502. doi: 10.1002/ar.1092210106. [DOI] [PubMed] [Google Scholar]
  32. WACHSTEIN M., MEISEL E. The distribution of histochemically demonstrable succinic dehydrogenase and of mitochondria in tongue and skeletal muscles. J Biophys Biochem Cytol. 1955 Nov 25;1(6):483–488. doi: 10.1083/jcb.1.6.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Williams K., Dhoot G. K. Heterogeneity and distribution of fast myosin heavy chains in some adult vertebrate skeletal muscles. Histochemistry. 1992 May;97(4):361–370. doi: 10.1007/BF00270039. [DOI] [PubMed] [Google Scholar]
  34. Yellin H. A histochemical study of muscle spindles and their relationship to extrafusal fiber types in the rat. Am J Anat. 1969 May;125(1):31–45. doi: 10.1002/aja.1001250103. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Anatomy are provided here courtesy of Anatomical Society of Great Britain and Ireland

RESOURCES