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. 1998 May;192(Pt 4):497–506. doi: 10.1046/j.1469-7580.1998.19240497.x

Surface ectodermal wound healing in the chick embryo

AARON LAWSON 1 ,✉, MARJORIE A ENGLAND 2
PMCID: PMC1467804  PMID: 9723977

Abstract

Wound healing has been studied in the surface ectoderm overlying the midbrain region of stages 16–20 chick embryos by light microscopy, scanning and transmission electron microscopy, and immunofluorescent techniques. The embryos were divided into 6 groups, i.e. stages 16–17 for groups I, V and VI, and stages 19–20 for groups II, III and IV. For groups I and II embryos, a longitudinal incision about 0.6 mm was made close to the dorsal midline and the embryos incubated for varying periods of time up to 24 h. To determine the role of actin in the process of healing, selected groups I and II embryos were stained with FITC phalloidin and the wound margins examined using a confocal microscope. Wounds of all embryos in group I and about 20% in group II healed completely within 24 h of reincubation. The process of healing involved a change in the shapes of the ectodermal cells at the wound ends. This appeared as a zipping-up of the wound from both ends. In about 80% of group II embryos where healing did not occur, wound gaping was marked. Intense actin staining (actin cable) was observed at the wound margins of groups I and II embryos suggesting that the actin purse-string mechanism may play a role during wound healing in this epithelial model. The role of tension in wound healing was also determined by placing 2 secondary wounds about 0.5–0.7 mm long close to, and at right angles to the ends of the primary wound in groups III and V embryos. The procedure decreased the tension within the ectodermal cells at the wound ends. Groups IV and VI embryos served as controls for groups III and V embryos, respectively. Healing of both primary and secondary wounds after reduction of tension was rapid. Most primary wounds in group V embryos healed completely within 3 h of reincubation and the rate of reepithelialisation after the reduction of tension was about 160% more than that in group VI (control) embryos. Similarly, most primary wounds in group III embryos were almost closed within 6 h of reincubation. Here, the rate of reepithelialisation was 80% more than that in group IV (controls). Thus tension is an important factor in wound healing in this model.

Keywords: Embryonic wound healing, ectoderm, actin

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Selected References

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  1. Bement W. M., Forscher P., Mooseker M. S. A novel cytoskeletal structure involved in purse string wound closure and cell polarity maintenance. J Cell Biol. 1993 May;121(3):565–578. doi: 10.1083/jcb.121.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brock J., Midwinter K., Lewis J., Martin P. Healing of incisional wounds in the embryonic chick wing bud: characterization of the actin purse-string and demonstration of a requirement for Rho activation. J Cell Biol. 1996 Nov;135(4):1097–1107. doi: 10.1083/jcb.135.4.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark B. J., Scothorne R. J. Variation in the response of chick embryos to incision of the roof plate of the neural tube at different developmental stages. J Anat. 1990 Feb;168:167–184. [PMC free article] [PubMed] [Google Scholar]
  4. Desmond M. E., Jacobson A. G. Embryonic brain enlargement requires cerebrospinal fluid pressure. Dev Biol. 1977 May;57(1):188–198. doi: 10.1016/0012-1606(77)90364-5. [DOI] [PubMed] [Google Scholar]
  5. England M. A., Cowper S. V. Wound healing in the early chick embryo studied by scanning electron microscopy. Anat Embryol (Berl) 1977 Dec 28;152(1):1–14. doi: 10.1007/BF00341431. [DOI] [PubMed] [Google Scholar]
  6. Kolega J. Effects of mechanical tension on protrusive activity and microfilament and intermediate filament organization in an epidermal epithelium moving in culture. J Cell Biol. 1986 Apr;102(4):1400–1411. doi: 10.1083/jcb.102.4.1400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lawson A., England M. A. Studies on wound healing in the neuroepithelium of the chick embryo. Anat Rec. 1992 Jun;233(2):291–300. doi: 10.1002/ar.1092330212. [DOI] [PubMed] [Google Scholar]
  8. Lawson A., England M. A. The effect of embryonic cerebrospinal fluid pressure and morphogenetic brain expansion on wound healing in the midbrain of the chick embryo. Anat Embryol (Berl) 1996 Jun;193(6):601–610. doi: 10.1007/BF00187932. [DOI] [PubMed] [Google Scholar]
  9. Mareel M. M., Vakaet L. C. Wound healing in the primitive deep layer of the young chick blastoderm. Virchows Arch B Cell Pathol. 1977 Dec 30;26(2):147–157. doi: 10.1007/BF02889544. [DOI] [PubMed] [Google Scholar]
  10. Martin P., Lewis J. Actin cables and epidermal movement in embryonic wound healing. Nature. 1992 Nov 12;360(6400):179–183. doi: 10.1038/360179a0. [DOI] [PubMed] [Google Scholar]
  11. Martin P., Nobes C. D. An early molecular component of the wound healing response in rat embryos--induction of c-fos protein in cells at the epidermal wound margin. Mech Dev. 1992 Sep;38(3):209–215. doi: 10.1016/0925-4773(92)90054-n. [DOI] [PubMed] [Google Scholar]
  12. Martin P., Nobes C., McCluskey J., Lewis J. Repair of excisional wounds in the embryo. Eye (Lond) 1994;8(Pt 2):155–160. doi: 10.1038/eye.1994.39. [DOI] [PubMed] [Google Scholar]
  13. Martin P. Wound healing--aiming for perfect skin regeneration. Science. 1997 Apr 4;276(5309):75–81. doi: 10.1126/science.276.5309.75. [DOI] [PubMed] [Google Scholar]
  14. Sherratt J. A., Lewis J. Stress-induced alignment of actin filaments and the mechanics of cytogel. Bull Math Biol. 1993 May;55(3):637–654. doi: 10.1007/BF02460654. [DOI] [PubMed] [Google Scholar]
  15. Smedley M. J., Stanisstreet M. Scanning electron microscopy of wound healing in rat embryos. J Embryol Exp Morphol. 1984 Oct;83:109–117. [PubMed] [Google Scholar]
  16. Stanisstreet M., Panayi M. Effects of colchicine, cytochalasin-B and papaverine on wound healing in Xenopus early embryos. Experientia. 1980 Sep 15;36(9):1110–1112. doi: 10.1007/BF01965999. [DOI] [PubMed] [Google Scholar]
  17. Stanisstreet M., Wakely J., England M. A. Scanning electron microscopy of wound healing in Xenopus and chicken embryos. J Embryol Exp Morphol. 1980 Oct;59:341–353. [PubMed] [Google Scholar]
  18. Whitby D. J., Ferguson M. W. Immunohistochemical localization of growth factors in fetal wound healing. Dev Biol. 1991 Sep;147(1):207–215. doi: 10.1016/s0012-1606(05)80018-1. [DOI] [PubMed] [Google Scholar]
  19. Whitby D. J., Ferguson M. W. The extracellular matrix of lip wounds in fetal, neonatal and adult mice. Development. 1991 Jun;112(2):651–668. doi: 10.1242/dev.112.2.651. [DOI] [PubMed] [Google Scholar]
  20. Whitby D. J., Longaker M. T., Harrison M. R., Adzick N. S., Ferguson M. W. Rapid epithelialisation of fetal wounds is associated with the early deposition of tenascin. J Cell Sci. 1991 Jul;99(Pt 3):583–586. doi: 10.1242/jcs.99.3.583. [DOI] [PubMed] [Google Scholar]

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