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. 1998 Jul;193(Pt 1):23–34. doi: 10.1046/j.1469-7580.1998.19310023.x

Collagen fibre arrangement in the tibial plateau articular cartilage of man and other mammalian species

M J KÄÄB 1 ,✉, I AP GWYNN 2 , H P NÖTZLI 3
PMCID: PMC1467820  PMID: 9758134

Abstract

Experimental animal models are frequently used to study articular cartilage, but the relevance to man remains problematic. In this study animal models were compared by examination of the collagen fibre arrangement in the medial tibial plateau of human, cow, pig, dog, sheep, rabbit and rat specimens. 24 cartilage samples from each species were prepared and maximum cartilage thickness in the central tibial plateau measured. Samples were fixed, dehydrated, freeze-fractured and imaged by scanning electron microscopy (SEM). At low magnification, 2 different arrangements of collagen fibres were observed: leaf-like (human, pig, dog) and columnar (cow, sheep, rabbit, rat). The porcine collagen structure was the most similar to that of man. This arrangement was consistent from the radial to the upper zones. Under higher magnification at the surface of the leaves, the collagen was more randomly oriented, whereas the columns consisted of parallel collagen fibrils. The maximum thickness of cartilage did not correlate with the type of collagen arrangement but was correlated with the body weight of the species (r=0.785). When using animal models for investigating human articular cartilage function or pathology, the differences in arrangement of collagen fibres in tibial plateau cartilage between laboratory animals should be considered especially if morphological evaluation is planned.

Keywords: Collagen, knee joint

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Selected References

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  1. Aston J. E., Bentley G. Repair of articular surfaces by allografts of articular and growth-plate cartilage. J Bone Joint Surg Br. 1986 Jan;68(1):29–35. doi: 10.1302/0301-620X.68B1.3941138. [DOI] [PubMed] [Google Scholar]
  2. Athanasiou K. A., Rosenwasser M. P., Buckwalter J. A., Malinin T. I., Mow V. C. Interspecies comparisons of in situ intrinsic mechanical properties of distal femoral cartilage. J Orthop Res. 1991 May;9(3):330–340. doi: 10.1002/jor.1100090304. [DOI] [PubMed] [Google Scholar]
  3. Biewener A. A. Scaling body support in mammals: limb posture and muscle mechanics. Science. 1989 Jul 7;245(4913):45–48. doi: 10.1126/science.2740914. [DOI] [PubMed] [Google Scholar]
  4. Bullough P. G., Yawitz P. S., Tafra L., Boskey A. L. Topographical variations in the morphology and biochemistry of adult canine tibial plateau articular cartilage. J Orthop Res. 1985;3(1):1–16. doi: 10.1002/jor.1100030101. [DOI] [PubMed] [Google Scholar]
  5. Clark J. M. The organisation of collagen fibrils in the superficial zones of articular cartilage. J Anat. 1990 Aug;171:117–130. [PMC free article] [PubMed] [Google Scholar]
  6. Clark J. M. The organization of collagen in cryofractured rabbit articular cartilage: a scanning electron microscopic study. J Orthop Res. 1985;3(1):17–29. doi: 10.1002/jor.1100030102. [DOI] [PubMed] [Google Scholar]
  7. Clark J. M. Variation of collagen fiber alignment in a joint surface: a scanning electron microscope study of the tibial plateau in dog, rabbit, and man. J Orthop Res. 1991 Mar;9(2):246–257. doi: 10.1002/jor.1100090213. [DOI] [PubMed] [Google Scholar]
  8. Clarke I. C. Articular cartilage: a review and scanning electron microscope study. 1. The interterritorial fibrillar architecture. J Bone Joint Surg Br. 1971 Nov;53(4):732–750. [PubMed] [Google Scholar]
  9. Hoch D. H., Grodzinsky A. J., Koob T. J., Albert M. L., Eyre D. R. Early changes in material properties of rabbit articular cartilage after meniscectomy. J Orthop Res. 1983;1(1):4–12. doi: 10.1002/jor.1100010102. [DOI] [PubMed] [Google Scholar]
  10. Jeffery A. K., Blunn G. W., Archer C. W., Bentley G. Three-dimensional collagen architecture in bovine articular cartilage. J Bone Joint Surg Br. 1991 Sep;73(5):795–801. doi: 10.1302/0301-620X.73B5.1894669. [DOI] [PubMed] [Google Scholar]
  11. Kobayashi S., Yonekubo S., Kurogouchi Y. Cryoscanning electron microscopic study of the surface amorphous layer of articular cartilage. J Anat. 1995 Oct;187(Pt 2):429–444. [PMC free article] [PubMed] [Google Scholar]
  12. Kobayashi S., Yonekubo S., Kurogouchi Y. Cryoscanning electron microscopy of loaded articular cartilage with special reference to the surface amorphous layer. J Anat. 1996 Apr;188(Pt 2):311–322. [PMC free article] [PubMed] [Google Scholar]
  13. Korkala O., Karaharju E., Grönblad M., Aalto K. Articular cartilage after meniscectomy. Rabbit knees studied with the scanning electron microscope. Acta Orthop Scand. 1984 Jun;55(3):273–277. doi: 10.3109/17453678408992355. [DOI] [PubMed] [Google Scholar]
  14. Lane J. M., Weiss C. Review of articular cartilage collagen research. Arthritis Rheum. 1975 Nov-Dec;18(6):553–562. doi: 10.1002/art.1780180605. [DOI] [PubMed] [Google Scholar]
  15. Marshall K. W., Chan A. D. Arthroscopic anterior cruciate ligament transection induces canine osteoarthritis. J Rheumatol. 1996 Feb;23(2):338–343. [PubMed] [Google Scholar]
  16. Minns R. J., Steven F. S. The collagen fibril organization in human articular cartilage. J Anat. 1977 Apr;123(Pt 2):437–457. [PMC free article] [PubMed] [Google Scholar]
  17. Moran M. E., Kim H. K., Salter R. B. Biological resurfacing of full-thickness defects in patellar articular cartilage of the rabbit. Investigation of autogenous periosteal grafts subjected to continuous passive motion. J Bone Joint Surg Br. 1992 Sep;74(5):659–667. doi: 10.1302/0301-620X.74B5.1527109. [DOI] [PubMed] [Google Scholar]
  18. Mow V. C., Holmes M. H., Lai W. M. Fluid transport and mechanical properties of articular cartilage: a review. J Biomech. 1984;17(5):377–394. doi: 10.1016/0021-9290(84)90031-9. [DOI] [PubMed] [Google Scholar]
  19. Quacci D., Dell'Orbo C., Diaz G. Collagen fibril ultrastructure alters after glycanolytic digestion. Ann Anat. 1992 Dec;174(6):569–574. doi: 10.1016/s0940-9602(11)80324-9. [DOI] [PubMed] [Google Scholar]
  20. Richards R. G., Käb M. J. Microwave-enhanced fixation of rabbit articular cartilage. J Microsc. 1996 Mar;181(Pt 3):269–276. doi: 10.1046/j.1365-2818.1996.125406.x. [DOI] [PubMed] [Google Scholar]
  21. Setton L. A., Mow V. C., Müller F. J., Pita J. C., Howell D. S. Mechanical properties of canine articular cartilage are significantly altered following transection of the anterior cruciate ligament. J Orthop Res. 1994 Jul;12(4):451–463. doi: 10.1002/jor.1100120402. [DOI] [PubMed] [Google Scholar]
  22. Simon W. H. Scale effects in animal joints. I. Articular cartilage thickness and compressive stress. Arthritis Rheum. 1970 May-Jun;13(3):244–256. doi: 10.1002/art.1780130305. [DOI] [PubMed] [Google Scholar]
  23. Simon W. H. Scale effects in animal joints. II. Thickness and elasticity in the deformability of articular cartilage. Arthritis Rheum. 1971 Jul-Aug;14(4):493–502. doi: 10.1002/art.1780140409. [DOI] [PubMed] [Google Scholar]
  24. Speer D. P., Dahners L. The collagenous architecture of articular cartilage. Correlation of scanning electron microscopy and polarized light microscopy observations. Clin Orthop Relat Res. 1979 Mar-Apr;(139):267–275. [PubMed] [Google Scholar]
  25. Stockwell R. A. The interrelationship of cell density and cartilage thickness in mammalian articular cartilage. J Anat. 1971 Sep;109(Pt 3):411–421. [PMC free article] [PubMed] [Google Scholar]
  26. Teshima R., Otsuka T., Takasu N., Yamagata N., Yamamoto K. Structure of the most superficial layer of articular cartilage. J Bone Joint Surg Br. 1995 May;77(3):460–464. [PubMed] [Google Scholar]
  27. Torzilli P. A., Dethmers D. A., Rose D. E., Schryuer H. F. Movement of interstitial water through loaded articular cartilage. J Biomech. 1983;16(3):169–179. doi: 10.1016/0021-9290(83)90124-0. [DOI] [PubMed] [Google Scholar]
  28. Wakitani S., Kimura T., Hirooka A., Ochi T., Yoneda M., Yasui N., Owaki H., Ono K. Repair of rabbit articular surfaces with allograft chondrocytes embedded in collagen gel. J Bone Joint Surg Br. 1989 Jan;71(1):74–80. doi: 10.1302/0301-620X.71B1.2915011. [DOI] [PubMed] [Google Scholar]
  29. White S. H., Ludkowski P. F., Goodfellow J. W. Anteromedial osteoarthritis of the knee. J Bone Joint Surg Br. 1991 Jul;73(4):582–586. doi: 10.1302/0301-620X.73B4.2071640. [DOI] [PubMed] [Google Scholar]
  30. Zambrano N. Z., Montes G. S., Shigihara K. M., Sanchez E. M., Junqueira L. C. Collagen arrangement in cartilages. Acta Anat (Basel) 1982;113(1):26–38. doi: 10.1159/000145534. [DOI] [PubMed] [Google Scholar]
  31. de Bont L. G., Liem R. S., Havinga P., Boering G., van der Korst J. Collagenous network in cartilage of human femoral condyles. A light microscopic and scanning electron microscopic study. Acta Anat (Basel) 1986;126(1):41–47. doi: 10.1159/000146184. [DOI] [PubMed] [Google Scholar]

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