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. 1998 Nov;193(Pt 4):587–597. doi: 10.1046/j.1469-7580.1998.19340587.x

Developmental changes in mucosubstances revealed by immunostaining with antimucus monoclonal antibodies and lectin staining in the epithelium lining the segment from gizzard to duodenum of the chick embryo

SUSUMU MATSUSHITA 1 ,✉, YASUO ISHII 2 ,* , SADAO YASUGI 2
PMCID: PMC1467884  PMID: 10029192

Abstract

The mucosubstances in the epithelium lining the segment from gizzard to duodenum during development of the chick embryo was studied histochemically using monoclonal antibodies against gizzard mucus and lectins, with attention to the regional differentiation of the epithelium in this segment. The anterior limit of epithelial CdxA mRNA expression detected by in situ hybridisation, which served as the position of the gizzard-duodenal boundary, was clearly found from d 3. Granules positive for some antibodies or lectins were found in the region ranging from the posterior part of the gizzard to the duodenum at d 3, which was followed by an increase in the number of granules and a gradual enlargement of the granule-positive area to the anterior part of the gizzard over 4–6 d. From d 4, the epithelia of the gizzard body and of the pyloric or duodenal region came to be differently stained with some antibodies or lectins. From d 10, each region showed a specific pattern of staining. The epithelia of the gizzard body and pyloric region contained abundant mucus granules with a different staining pattern. In the duodenum the number of stained granules was low except in occasional goblet cells. Thus the epithelia of the gizzard body, pyloric region and duodenum may produce different mucosubstances and the regional differentiation in these epithelia may start at rather early stages soon after the formation of digestive tube.

Keywords: Glycoconjugates, gastrointestinal tract, gizzard-duodenal boundary

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Selected References

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  1. AITKEN R. N. A histochemical study of the stomach and intestine of the chicken. J Anat. 1958 Jul;92(3):453–466. [PMC free article] [PubMed] [Google Scholar]
  2. Allison R. T. The effects of various fixatives on subsequent lectin binding to tissue sections. Histochem J. 1987 Feb;19(2):65–74. doi: 10.1007/BF01682750. [DOI] [PubMed] [Google Scholar]
  3. Brown K. M. Sucrase activity in the intestine of the chick: normal development and influence of hydrocortisone, actinomycin D, cycloheximide, and puromycin. J Exp Zool. 1971 Aug;177(4):493–506. doi: 10.1002/jez.1401770410. [DOI] [PubMed] [Google Scholar]
  4. Callaghan J. M., Toh B. H., Pettitt J. M., Humphris D. C., Gleeson P. A. Poly-N-acetyllactosamine-specific tomato lectin interacts with gastric parietal cells. Identification of a tomato-lectin binding 60-90 X 10(3) Mr membrane glycoprotein of tubulovesicles. J Cell Sci. 1990 Apr;95(Pt 4):563–576. doi: 10.1242/jcs.95.4.563. [DOI] [PubMed] [Google Scholar]
  5. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  6. Gheri G., Bryk S. G., Sgambati E. Identification of sugar residues in secretory glycoconjugates of the lining and glandular epithelium of the chick embryo gizzard using lectin histochemistry. Acta Histochem. 1994 Dec;96(4):387–397. doi: 10.1016/S0065-1281(11)80023-1. [DOI] [PubMed] [Google Scholar]
  7. Haffen K., Kedinger M., Simon-Assmann P. Mesenchyme-dependent differentiation of epithelial progenitor cells in the gut. J Pediatr Gastroenterol Nutr. 1987 Jan-Feb;6(1):14–23. doi: 10.1097/00005176-198701000-00005. [DOI] [PubMed] [Google Scholar]
  8. Hijmans J. C., McCarty K. S. Induction of invertase activity by hydrocortisone in chick embryo duodenum cultures. Proc Soc Exp Biol Med. 1966 Dec;123(3):633–637. doi: 10.3181/00379727-123-31563. [DOI] [PubMed] [Google Scholar]
  9. Ishii Y., Fukuda K., Saiga H., Matsushita S., Yasugi S. Early specification of intestinal epithelium in the chicken embryo: a study on the localization and regulation of CdxA expression. Dev Growth Differ. 1997 Oct;39(5):643–653. doi: 10.1046/j.1440-169x.1997.t01-4-00012.x. [DOI] [PubMed] [Google Scholar]
  10. Ishizuya-Oka A., Mizuno T. Intestinal cytodifferentiation in vitro of chick stomach endoderm induced by the duodenal mesenchyme. J Embryol Exp Morphol. 1984 Aug;82:163–176. [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Matsushita S. Development of sucrase in the chick small intestine. J Exp Zool. 1985 Mar;233(3):377–383. doi: 10.1002/jez.1402330306. [DOI] [PubMed] [Google Scholar]
  13. Mizuno T., Yasugi S. Susceptibility of epithelia to directive influences of mesenchymes during organogenesis: uncoupling of morphogenesis and cytodifferentiation. Cell Differ Dev. 1990 Sep;31(3):151–159. doi: 10.1016/0922-3371(90)90117-f. [DOI] [PubMed] [Google Scholar]
  14. Sgambati E., Gheri Bryk S., Gheri G. Histochemical study of the epithelial mucins in the gizzard of the chick embryo. Ital J Anat Embryol. 1996 Jul-Sep;101(3):173–185. [PubMed] [Google Scholar]
  15. TONER P. G. DEVELOPMENT OF THE ACID SECRETORY POTENTIAL IN THE CHICK EMBRYO PROVENTRICULUS. J Anat. 1965 Apr;99:389–398. [PMC free article] [PubMed] [Google Scholar]
  16. Toner P. G. Ultrastructure of the developing gizzard epithelium in the chick embryo. Z Zellforsch Mikrosk Anat. 1966;73(2):220–233. doi: 10.1007/BF00334865. [DOI] [PubMed] [Google Scholar]
  17. VAN ALTEN P. J., FENNELL R. A. Histogenesis and histochemistry of the digestive tract of chick embryos. Anat Rec. 1957 Apr;127(4):677–695. doi: 10.1002/ar.1091270405. [DOI] [PubMed] [Google Scholar]
  18. Wu A. M., Shen F., Herp A., Wu J. H. Interaction of hamster submaxillary sialyl-Tn and Tn glycoproteins with Gal, GalNAc and GlcNAc specific lectins. Mol Immunol. 1994 Apr;31(6):485–490. doi: 10.1016/0161-5890(94)90067-1. [DOI] [PubMed] [Google Scholar]
  19. Wu A. M., WU J. H., Watkins W. M., Chen C. P., Tsai M. C. Binding properties of a blood group Le(a+) active sialoglycoprotein, purified from human ovarian cyst, with applied lectins. Biochim Biophys Acta. 1996 Jun 7;1316(2):139–144. doi: 10.1016/0925-4439(96)00016-6. [DOI] [PubMed] [Google Scholar]

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