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. 1999 Jul;195(Pt 1):39–44. doi: 10.1046/j.1469-7580.1999.19510039.x

Temporal expression of the matrix metalloproteinase MMP-2 correlates with fibronectin immunoreactivity during the development of the vascular system in the chick embryo chorioallantoic membrane

DOMENICO RIBATTI 1 ,✉, BEATRICE NICO 1 , ANGELO VACCA 2 , MONICA IURLARO 2 , LUISA RONCALI 1
PMCID: PMC1467963  PMID: 10473291

Abstract

In this study we have examined in the chick embryo chorioallantoic membrane (ChAM) the expression of the matrix metalloproteinase 2 (MMP-2) and have correlated this parameter with the expansion of the ChAM vasculature and with the expression of 3 extracellular matrix components (fibronectin, laminin and type IV collagen), which differentially modulate angiogenesis. In the early phases of ChAM development, between d 6 and d 8 of incubation, when the increase of the ChAM vasculature is maximal, higher values of MMP-2 and, respectively, of fibronectin immunoreactive area, are detectable. These results indicate that MMP-2 activity and fibronectin expression are 2 strictly related components of angiogenesis occurring in vivo.

Keywords: Angiogenesis, metalloproteinase 2

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Selected References

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  1. Albini A., Iwamoto Y., Kleinman H. K., Martin G. R., Aaronson S. A., Kozlowski J. M., McEwan R. N. A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 1987 Jun 15;47(12):3239–3245. [PubMed] [Google Scholar]
  2. Ausprunk D. H., Knighton D. R., Folkman J. Differentiation of vascular endothelium in the chick chorioallantois: a structural and autoradiographic study. Dev Biol. 1974 Jun;38(2):237–248. doi: 10.1016/0012-1606(74)90004-9. [DOI] [PubMed] [Google Scholar]
  3. Aznavoorian S., Murphy A. N., Stetler-Stevenson W. G., Liotta L. A. Molecular aspects of tumor cell invasion and metastasis. Cancer. 1993 Feb 15;71(4):1368–1383. doi: 10.1002/1097-0142(19930215)71:4<1368::aid-cncr2820710432>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  4. Brooks P. C., Strömblad S., Sanders L. C., von Schalscha T. L., Aimes R. T., Stetler-Stevenson W. G., Quigley J. P., Cheresh D. A. Localization of matrix metalloproteinase MMP-2 to the surface of invasive cells by interaction with integrin alpha v beta 3. Cell. 1996 May 31;85(5):683–693. doi: 10.1016/s0092-8674(00)81235-0. [DOI] [PubMed] [Google Scholar]
  5. Burton G. J., Palmer M. E. Development of the chick chorioallantoic capillary plexus under normoxic and normobaric hypoxic and hyperoxic conditions: a morphometric study. J Exp Zool. 1992 Jun 1;262(3):291–298. doi: 10.1002/jez.1402620309. [DOI] [PubMed] [Google Scholar]
  6. Burton G. J., Palmer M. E. The chorioallantoic capillary plexus of the chicken egg: a microvascular corrosion casting study. Scanning Microsc. 1989 Jun;3(2):549–558. [PubMed] [Google Scholar]
  7. Chen W. T., Chen J. M., Mueller S. C. Coupled expression and colocalization of 140K cell adhesion molecules, fibronectin, and laminin during morphogenesis and cytodifferentiation of chick lung cells. J Cell Biol. 1986 Sep;103(3):1073–1090. doi: 10.1083/jcb.103.3.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DeFouw D. O., Rizzo V. J., Steinfeld R., Feinberg R. N. Mapping of the microcirculation in the chick chorioallantoic membrane during normal angiogenesis. Microvasc Res. 1989 Sep;38(2):136–147. doi: 10.1016/0026-2862(89)90022-8. [DOI] [PubMed] [Google Scholar]
  9. Form D. M., Pratt B. M., Madri J. A. Endothelial cell proliferation during angiogenesis. In vitro modulation by basement membrane components. Lab Invest. 1986 Nov;55(5):521–530. [PubMed] [Google Scholar]
  10. Iruela-Arispe M. L., Lane T. F., Redmond D., Reilly M., Bolender R. P., Kavanagh T. J., Sage E. H. Expression of SPARC during development of the chicken chorioallantoic membrane: evidence for regulated proteolysis in vivo. Mol Biol Cell. 1995 Mar;6(3):327–343. doi: 10.1091/mbc.6.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Karelina T. V., Goldberg G. I., Eisen A. Z. Matrix metalloproteinases in blood vessel development in human fetal skin and in cutaneous tumors. J Invest Dermatol. 1995 Sep;105(3):411–417. doi: 10.1111/1523-1747.ep12321097. [DOI] [PubMed] [Google Scholar]
  12. Matrisian L. M. The matrix-degrading metalloproteinases. Bioessays. 1992 Jul;14(7):455–463. doi: 10.1002/bies.950140705. [DOI] [PubMed] [Google Scholar]
  13. Mayer B. W., Jr, Hay E. D., Hynes R. O. Immunocytochemical localization of fibronectin in embryonic chick trunk and area vasculosa. Dev Biol. 1981 Mar;82(2):267–286. doi: 10.1016/0012-1606(81)90451-6. [DOI] [PubMed] [Google Scholar]
  14. Mignatti P., Rifkin D. B. Biology and biochemistry of proteinases in tumor invasion. Physiol Rev. 1993 Jan;73(1):161–195. doi: 10.1152/physrev.1993.73.1.161. [DOI] [PubMed] [Google Scholar]
  15. Nicosia R. F., Madri J. A. The microvascular extracellular matrix. Developmental changes during angiogenesis in the aortic ring-plasma clot model. Am J Pathol. 1987 Jul;128(1):78–90. [PMC free article] [PubMed] [Google Scholar]
  16. Pepper M. S., Montesano R., Mandriota S. J., Orci L., Vassalli J. D. Angiogenesis: a paradigm for balanced extracellular proteolysis during cell migration and morphogenesis. Enzyme Protein. 1996;49(1-3):138–162. doi: 10.1159/000468622. [DOI] [PubMed] [Google Scholar]
  17. Ribatti D. A morphometric study of the expansion of the chick area vasculosa in shell-less culture. J Anat. 1995 Jun;186(Pt 3):639–644. [PMC free article] [PubMed] [Google Scholar]
  18. Ribatti D., Bertossi M., Nico B., Vacca A., Ria R., Riva A., Roncali L., Presta M. Role of basic fibroblast growth factor in the formation of the capillary plexus in the chick embryo chorioallantoic membrane. An in situ hybridization, immunohistochemical and ultrastructural study. J Submicrosc Cytol Pathol. 1998 Jan;30(1):127–136. [PubMed] [Google Scholar]
  19. Ribatti D., Nico B., Bertossi M., Roncali L., Presta M. Basic fibroblast growth factor-induced angiogenesis in the chick embryo chorioallantoic membrane: an electron microscopy study. Microvasc Res. 1997 Mar;53(2):187–190. doi: 10.1006/mvre.1996.1993. [DOI] [PubMed] [Google Scholar]
  20. Ribatti D., Urbinati C., Nico B., Rusnati M., Roncali L., Presta M. Endogenous basic fibroblast growth factor is implicated in the vascularization of the chick embryo chorioallantoic membrane. Dev Biol. 1995 Jul;170(1):39–49. doi: 10.1006/dbio.1995.1193. [DOI] [PubMed] [Google Scholar]
  21. Ribatti D., Vacca A., Costantino F., Minischetti M., Locci P., Becchetti E., Roncali L., Dammacco F. Exogenous heparin induces fibronectin overexpression parallel to angiogenesis in the extracellular matrix of the chick embryo chorioallantoic membrane. Tissue Cell. 1997 Apr;29(2):131–136. doi: 10.1016/s0040-8166(97)80014-6. [DOI] [PubMed] [Google Scholar]
  22. Ribatti D., Vacca A., Nico B., Fanelli M., Roncali L., Dammacco F. Angiogenesis spectrum in the stroma of B-cell non-Hodgkin's lymphomas. An immunohistochemical and ultrastructural study. Eur J Haematol. 1996 Jan-Feb;56(1-2):45–53. doi: 10.1111/j.1600-0609.1996.tb00293.x. [DOI] [PubMed] [Google Scholar]
  23. Risau W., Lemmon V. Changes in the vascular extracellular matrix during embryonic vasculogenesis and angiogenesis. Dev Biol. 1988 Feb;125(2):441–450. doi: 10.1016/0012-1606(88)90225-4. [DOI] [PubMed] [Google Scholar]
  24. Sariola H., Kuusela P., Ekblom P. Cellular origin of fibronectin in interspecies hybrid kidneys. J Cell Biol. 1984 Dec;99(6):2099–2107. doi: 10.1083/jcb.99.6.2099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schlatter P., König M. F., Karlsson L. M., Burri P. H. Quantitative study of intussusceptive capillary growth in the chorioallantoic membrane (CAM) of the chicken embryo. Microvasc Res. 1997 Jul;54(1):65–73. doi: 10.1006/mvre.1997.2022. [DOI] [PubMed] [Google Scholar]
  26. Shumko J. Z., Defouw D. O., Feinberg R. N. Vascular histodifferentiation in the chick chorioallantoic membrane: a morphometric study. Anat Rec. 1988 Feb;220(2):179–189. doi: 10.1002/ar.1092200209. [DOI] [PubMed] [Google Scholar]
  27. Vacca A., Di Loreto M., Ribatti D., Di Stefano R., Gadaleta-Caldarola G., Iodice G., Caloro D., Dammacco F. Bone marrow of patients with active multiple myeloma: angiogenesis and plasma cell adhesion molecules LFA-1, VLA-4, LAM-1, and CD44. Am J Hematol. 1995 Sep;50(1):9–14. doi: 10.1002/ajh.2830500103. [DOI] [PubMed] [Google Scholar]

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