Abstract
The thigh muscles of New Zealand White (NZW) rabbits are frequently used in experimental surgery, particularly for evaluation after reinnervation or ischaemia. Although histomorphometric analyses are regularly performed, morphological data for untreated thigh muscles in previously unoperated animals are not available. Specimens from the rectus femoris (RF), vastus medialis (VM) and adductor magnus (AM) muscles from both thighs were harvested in 7 untreated rabbits and were processed for histomorphometric evaluation. The right RF and VM were harvested in a further 5 rabbit hindlimbs after experimental denervation and reinnervation of the contralateral RF and subsequently processed for histomorphometric analysis. Muscle fibre type distribution, diameter and connective tissue content were evaluated on serial transverse cryosections reacted for ATPase and NADH tetrazolium reductase activity and statistical analysis was performed. In all untreated animals RF revealed the highest proportion of type I muscle fibres (right: 8.4±4%, left: 11.4±4.9%), whereas VM showed the highest percentage of IIa fibres (right: 31.9±5.5%, left: 28.3±7.8%) and AM the highest proportion of IIb/d fibres (right: 80.5±8.6%, left: 84.4±6.3%). Fibre type distribution and diameter in rabbits after contralateral experimental operations revealed a statistically significant difference from the data obtained in bilaterally untreated animals. Knowledge of the morphology of untreated muscles is fundamental to the understanding of changes induced by intervention to the ipsi and/or contralateral thigh muscles.
Keywords: Myology, muscle fibre type distribution
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- Dahl H. A., Roald L. How unequivocal is the muscle fibre type concept? Anat Embryol (Berl) 1991;184(3):269–273. doi: 10.1007/BF01673261. [DOI] [PubMed] [Google Scholar]
- Frey M., Giovanoli P., Deutinger M., Meuli-Simmen C. Scutuloauricularis muscle of the rabbit: a new model for free functional muscle transplantation in the mimic system. Microsurgery. 1996;17(5):254–258. doi: 10.1002/(SICI)1098-2752(1996)17:5<254::AID-MICR3>3.0.CO;2-J. [DOI] [PubMed] [Google Scholar]
- Frey M., Giovanoli P., Meuli-Simmen C. The qualification of different free muscle transplants to reconstruct mimic function: an experimental study in rabbits. Plast Reconstr Surg. 1998 Jun;101(7):1774–1783. doi: 10.1097/00006534-199806000-00002. [DOI] [PubMed] [Google Scholar]
- Fuentes I., Cobos A. R., Segade L. A. Muscle fibre types and their distribution in the biceps and triceps brachii of the rat and rabbit. J Anat. 1998 Feb;192(Pt 2):203–210. doi: 10.1046/j.1469-7580.1998.19220203.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guth L., Samaha F. J. Procedure for the histochemical demonstration of actomyosin ATPase. Exp Neurol. 1970 Aug;28(2):365–367. [PubMed] [Google Scholar]
- Hickey M. J., Hurley J. V., Angel M. F., O'Brien B. M. The response of the rabbit rectus femoris muscle to ischemia and reperfusion. J Surg Res. 1992 Oct;53(4):369–377. doi: 10.1016/0022-4804(92)90063-6. [DOI] [PubMed] [Google Scholar]
- Huk I., Nanobashvili J., Neumayer C., Punz A., Mueller M., Afkhampour K., Mittlboeck M., Losert U., Polterauer P., Roth E. L-arginine treatment alters the kinetics of nitric oxide and superoxide release and reduces ischemia/reperfusion injury in skeletal muscle. Circulation. 1997 Jul 15;96(2):667–675. doi: 10.1161/01.cir.96.2.667. [DOI] [PubMed] [Google Scholar]
- Hämäläinen N., Pette D. The histochemical profiles of fast fiber types IIB, IID, and IIA in skeletal muscles of mouse, rat, and rabbit. J Histochem Cytochem. 1993 May;41(5):733–743. doi: 10.1177/41.5.8468455. [DOI] [PubMed] [Google Scholar]
- Koller R., Rab M., Todoroff B. P., Neumayer C., Haslik W., Stöhr H. G., Frey M. The influence of the graft length on the functional and morphological result after nerve grafting: an experimental study in rabbits. Br J Plast Surg. 1997 Dec;50(8):609–614. doi: 10.1016/s0007-1226(97)90506-3. [DOI] [PubMed] [Google Scholar]
- Lexell J., Jarvis J. C., Currie J., Downham D. Y., Salmons S. Fibre type composition of rabbit tibialis anterior and extensor digitorum longus muscles. J Anat. 1994 Aug;185(Pt 1):95–101. [PMC free article] [PubMed] [Google Scholar]
- Nygaard E., Sanchez J. Intramuscular variation of fiber types in the brachial biceps and the lateral vastus muscles of elderly men: how representative is a small biopsy sample? Anat Rec. 1982 Aug;203(4):451–459. doi: 10.1002/ar.1092030404. [DOI] [PubMed] [Google Scholar]
- Reichmann H., Srihari T., Pette D. Ipsi- and contralateral fibre transformations by cross-reinnervation. A principle of symmetry. Pflugers Arch. 1983 May;397(3):202–208. doi: 10.1007/BF00584358. [DOI] [PubMed] [Google Scholar]
- Srihari T., Seedorf U., Pette D. Ipsi-and contralateral changes in rabbit soleus myosins by cross-reinnervation. Pflugers Arch. 1981 Jun;390(3):246–249. doi: 10.1007/BF00658269. [DOI] [PubMed] [Google Scholar]