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. 2001 Apr;198(Pt 4):385–398. doi: 10.1046/j.1469-7580.2001.19840385.x

Reproduction in the Noughties: will the scientists have all the fun?

MARTIN H JOHNSON 1,✉
PMCID: PMC1468240  PMID: 11327201

Abstract

The past 20–30 years have seen major advances in our understanding of human reproduction and in our ability to manipulate it, as well as major social changes in human reproductive and sexual attitudes. Many of these advances and changes developed out of the first successful in vitro fertilisation (IVF) of the human oocyte. It is also the case that 30 years ago few foresaw what was to come, and many were at best doubtful and often were very critical of the scientific work which led to human IVF and to many of the subsequent developments. This lack of foresight provides us with a lesson about the dangers that we face in looking forward and attempting to predict the future. This review will try to convey, not comprehensively but through examples, the flavour of current activities in Assisted Reproduction clinics and research laboratories around the world and what is being talked about for the future in respect of emergent patient demands and anticipated clinical needs. This clinically driven approach will form the basis for consideration of some underlying scientific aspects of reproductive research, some of the ethicolegal issues that may arise, and the implications of this anticipated future for our current approaches to medical education. The future will be considered not simply in terms of the New Reproduction itself but also in its interaction with the opportunities and challenges presented by the New Genetics. It is perhaps in the interaction between these two fields of endeavour that some of the most difficult challenges ahead lie.

Keywords: Assisted reproduction, pre-implantation genetic diagnosis, cloning, HFEA, bioethics

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Selected References

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  1. Baguisi A., Behboodi E., Melican D. T., Pollock J. S., Destrempes M. M., Cammuso C., Williams J. L., Nims S. D., Porter C. A., Midura P. Production of goats by somatic cell nuclear transfer. Nat Biotechnol. 1999 May;17(5):456–461. doi: 10.1038/8632. [DOI] [PubMed] [Google Scholar]
  2. Balaban B., Urman B., Isiklar A., Alatas C., Aksoy S., Mercan R., Nuhoglu A. Progression to the blastocyst stage of embryos derived from testicular round spermatids. Hum Reprod. 2000 Jun;15(6):1377–1382. doi: 10.1093/humrep/15.6.1377. [DOI] [PubMed] [Google Scholar]
  3. Bolton V. N. Controversies and opinions in embryo culture: two- to four-cell transfer vs blastocyst. J Assist Reprod Genet. 1992 Dec;9(6):506–508. doi: 10.1007/BF01204245. [DOI] [PubMed] [Google Scholar]
  4. Brinster R. L., Avarbock M. R. Germline transmission of donor haplotype following spermatogonial transplantation. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11303–11307. doi: 10.1073/pnas.91.24.11303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brinster R. L., Zimmermann J. W. Spermatogenesis following male germ-cell transplantation. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11298–11302. doi: 10.1073/pnas.91.24.11298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bush T. G., Savidge T. C., Freeman T. C., Cox H. J., Campbell E. A., Mucke L., Johnson M. H., Sofroniew M. V. Fulminant jejuno-ileitis following ablation of enteric glia in adult transgenic mice. Cell. 1998 Apr 17;93(2):189–201. doi: 10.1016/s0092-8674(00)81571-8. [DOI] [PubMed] [Google Scholar]
  7. Campbell K. H., McWhir J., Ritchie W. A., Wilmut I. Sheep cloned by nuclear transfer from a cultured cell line. Nature. 1996 Mar 7;380(6569):64–66. doi: 10.1038/380064a0. [DOI] [PubMed] [Google Scholar]
  8. Clouthier D. E., Avarbock M. R., Maika S. D., Hammer R. E., Brinster R. L. Rat spermatogenesis in mouse testis. Nature. 1996 May 30;381(6581):418–421. doi: 10.1038/381418a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cohen J., Scott R., Alikani M., Schimmel T., Munné S., Levron J., Wu L., Brenner C., Warner C., Willadsen S. Ooplasmic transfer in mature human oocytes. Mol Hum Reprod. 1998 Mar;4(3):269–280. doi: 10.1093/molehr/4.3.269. [DOI] [PubMed] [Google Scholar]
  10. Delhanty J. D., Harper J. C., Ao A., Handyside A. H., Winston R. M. Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients. Hum Genet. 1997 Jun;99(6):755–760. doi: 10.1007/s004390050443. [DOI] [PubMed] [Google Scholar]
  11. Doherty A. S., Mann M. R., Tremblay K. D., Bartolomei M. S., Schultz R. M. Differential effects of culture on imprinted H19 expression in the preimplantation mouse embryo. Biol Reprod. 2000 Jun;62(6):1526–1535. doi: 10.1095/biolreprod62.6.1526. [DOI] [PubMed] [Google Scholar]
  12. Edirisinghe W. R., Wales R. G., Pike I. L. Degradation of biochemical pools labelled with [14C]glucose during culture of 8-cell and morula--early blastocyst-stage mouse embryos in vitro and in vivo. J Reprod Fertil. 1984 Sep;72(1):59–65. doi: 10.1530/jrf.0.0720059. [DOI] [PubMed] [Google Scholar]
  13. Edwards B. E., Gearhart J. D., Wallach E. E. The human pluripotent stem cell: impact on medicine and society. Fertil Steril. 2000 Jul;74(1):1–7. doi: 10.1016/s0015-0282(00)00583-5. [DOI] [PubMed] [Google Scholar]
  14. Eppig J. J., O'Brien M. J. Comparison of preimplantation developmental competence after mouse oocyte growth and development in vitro and in vivo. Theriogenology. 1998 Jan 15;49(2):415–422. doi: 10.1016/s0093-691x(97)00413-5. [DOI] [PubMed] [Google Scholar]
  15. Eppig J. J., Wigglesworth K. Development of mouse and rat oocytes in chimeric reaggregated ovaries after interspecific exchange of somatic and germ cell components. Biol Reprod. 2000 Oct;63(4):1014–1023. doi: 10.1095/biolreprod63.4.1014. [DOI] [PubMed] [Google Scholar]
  16. França L. R., Ogawa T., Avarbock M. R., Brinster R. L., Russell L. D. Germ cell genotype controls cell cycle during spermatogenesis in the rat. Biol Reprod. 1998 Dec;59(6):1371–1377. doi: 10.1095/biolreprod59.6.1371. [DOI] [PubMed] [Google Scholar]
  17. Gardner D. K., Schoolcraft W. B. Culture and transfer of human blastocysts. Curr Opin Obstet Gynecol. 1999 Jun;11(3):307–311. doi: 10.1097/00001703-199906000-00013. [DOI] [PubMed] [Google Scholar]
  18. Handyside A. H., Kontogianni E. H., Hardy K., Winston R. M. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature. 1990 Apr 19;344(6268):768–770. doi: 10.1038/344768a0. [DOI] [PubMed] [Google Scholar]
  19. Hassold T., Hunt P. A., Sherman S. Trisomy in humans: incidence, origin and etiology. Curr Opin Genet Dev. 1993 Jun;3(3):398–403. doi: 10.1016/0959-437x(93)90111-2. [DOI] [PubMed] [Google Scholar]
  20. Johnson M. H. Genetics, the free market and reproductive medicine. Hum Reprod. 1997 Mar;12(3):408–410. doi: 10.1093/oxfordjournals.humrep.a019573. [DOI] [PubMed] [Google Scholar]
  21. Johnson M. H., Kubiak J. Z. Legal confusion over 'cloning' risks throwing baby out with bathwater. Nature. 2000 Oct 5;407(6804):559–559. doi: 10.1038/35036747. [DOI] [PubMed] [Google Scholar]
  22. Johnson M. H. Should the use of assisted reproduction techniques be deregulated? The UK experience: options for change. Hum Reprod. 1998 Jul;13(7):1769–1776. doi: 10.1093/humrep/13.7.1769. [DOI] [PubMed] [Google Scholar]
  23. Johnson M. H. The medical ethics of paid egg sharing in the UK. Hum Reprod. 1999 Jul;14(7):1912–1918. doi: 10.1093/humrep/14.7.1912. [DOI] [PubMed] [Google Scholar]
  24. Johnson M. Cloning humans? Bioessays. 1997 Aug;19(8):737–739. doi: 10.1002/bies.950190815. [DOI] [PubMed] [Google Scholar]
  25. Kato Y., Rideout W. M., 3rd, Hilton K., Barton S. C., Tsunoda Y., Surani M. A. Developmental potential of mouse primordial germ cells. Development. 1999 May;126(9):1823–1832. doi: 10.1242/dev.126.9.1823. [DOI] [PubMed] [Google Scholar]
  26. McCreath K. J., Howcroft J., Campbell K. H., Colman A., Schnieke A. E., Kind A. J. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature. 2000 Jun 29;405(6790):1066–1069. doi: 10.1038/35016604. [DOI] [PubMed] [Google Scholar]
  27. McLaren A. Sex chimaerism and germ cell distribution in a series of chimaeric mice. J Embryol Exp Morphol. 1975 Feb;33(1):205–216. [PubMed] [Google Scholar]
  28. Nagano M., Shinohara T., Avarbock M. R., Brinster R. L. Retrovirus-mediated gene delivery into male germ line stem cells. FEBS Lett. 2000 Jun 9;475(1):7–10. doi: 10.1016/s0014-5793(00)01606-9. [DOI] [PubMed] [Google Scholar]
  29. Nasr-Esfahani M. H., Aitken J. R., Johnson M. H. Hydrogen peroxide levels in mouse oocytes and early cleavage stage embryos developed in vitro or in vivo. Development. 1990 Jun;109(2):501–507. doi: 10.1242/dev.109.2.501. [DOI] [PubMed] [Google Scholar]
  30. Ogawa T., Dobrinski I., Avarbock M. R., Brinster R. L. Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes. Biol Reprod. 1999 Feb;60(2):515–521. doi: 10.1095/biolreprod60.2.515. [DOI] [PubMed] [Google Scholar]
  31. Oktay K., Newton H., Gosden R. G. Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice. Fertil Steril. 2000 Mar;73(3):599–603. doi: 10.1016/s0015-0282(99)00548-8. [DOI] [PubMed] [Google Scholar]
  32. Perry A. C., Wakayama T., Kishikawa H., Kasai T., Okabe M., Toyoda Y., Yanagimachi R. Mammalian transgenesis by intracytoplasmic sperm injection. Science. 1999 May 14;284(5417):1180–1183. doi: 10.1126/science.284.5417.1180. [DOI] [PubMed] [Google Scholar]
  33. Pickering S. J., Taylor A., Johnson M. H., Braude P. R. An analysis of multinucleated blastomere formation in human embryos. Hum Reprod. 1995 Jul;10(7):1912–1922. doi: 10.1093/oxfordjournals.humrep.a136206. [DOI] [PubMed] [Google Scholar]
  34. Polejaeva I. A., Chen S. H., Vaught T. D., Page R. L., Mullins J., Ball S., Dai Y., Boone J., Walker S., Ayares D. L. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature. 2000 Sep 7;407(6800):86–90. doi: 10.1038/35024082. [DOI] [PubMed] [Google Scholar]
  35. Reik W., Römer I., Barton S. C., Surani M. A., Howlett S. K., Klose J. Adult phenotype in the mouse can be affected by epigenetic events in the early embryo. Development. 1993 Nov;119(3):933–942. doi: 10.1242/dev.119.3.933. [DOI] [PubMed] [Google Scholar]
  36. Rideout W. M., 3rd, Wakayama T., Wutz A., Eggan K., Jackson-Grusby L., Dausman J., Yanagimachi R., Jaenisch R. Generation of mice from wild-type and targeted ES cells by nuclear cloning. Nat Genet. 2000 Feb;24(2):109–110. doi: 10.1038/72753. [DOI] [PubMed] [Google Scholar]
  37. Rossant J., Spence A. Chimeras and mosaics in mouse mutant analysis. Trends Genet. 1998 Sep;14(9):358–363. doi: 10.1016/s0168-9525(98)01552-2. [DOI] [PubMed] [Google Scholar]
  38. Steptoe P. C., Edwards R. G. Birth after the reimplantation of a human embryo. Lancet. 1978 Aug 12;2(8085):366–366. doi: 10.1016/s0140-6736(78)92957-4. [DOI] [PubMed] [Google Scholar]
  39. Sztein J. M., O'Brien M. J., Farley J. S., Mobraaten L. E., Eppig J. J. Rescue of oocytes from antral follicles of cryopreserved mouse ovaries: competence to undergo maturation, embryogenesis, and development to term. Hum Reprod. 2000 Mar;15(3):567–571. doi: 10.1093/humrep/15.3.567. [DOI] [PubMed] [Google Scholar]
  40. Tamashiro K. L., Wakayama T., Blanchard R. J., Blanchard D. C., Yanagimachi R. Postnatal growth and behavioral development of mice cloned from adult cumulus cells. Biol Reprod. 2000 Jul;63(1):328–334. doi: 10.1095/biolreprod63.1.328. [DOI] [PubMed] [Google Scholar]
  41. Tilghman S. M. The sins of the fathers and mothers: genomic imprinting in mammalian development. Cell. 1999 Jan 22;96(2):185–193. doi: 10.1016/s0092-8674(00)80559-0. [DOI] [PubMed] [Google Scholar]
  42. Van Blerkom J., Barton S. C., Johnson M. H. Molecular differentiation in the preimplantation mouse embryo. Nature. 1976 Jan 29;259(5541):319–321. doi: 10.1038/259319a0. [DOI] [PubMed] [Google Scholar]
  43. Verlinsky Y., Rechitsky S., Verlinsky O., Ivachnenko V., Lifchez A., Kaplan B., Moise J., Valle J., Borkowski A., Nefedova J. Prepregnancy testing for single-gene disorders by polar body analysis. Genet Test. 1999;3(2):185–190. doi: 10.1089/gte.1999.3.185. [DOI] [PubMed] [Google Scholar]
  44. Vernet M., Cavard C., Zider A., Fergelot P., Grimber G., Briand P. In vitro manipulation of early mouse embryos induces HIV1-LTRlacZ transgene expression. Development. 1993 Dec;119(4):1293–1300. doi: 10.1242/dev.119.4.1293. [DOI] [PubMed] [Google Scholar]
  45. Wells D. N., Misica P. M., Tervit H. R. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol Reprod. 1999 Apr;60(4):996–1005. doi: 10.1095/biolreprod60.4.996. [DOI] [PubMed] [Google Scholar]
  46. Young L. E., Sinclair K. D., Wilmut I. Large offspring syndrome in cattle and sheep. Rev Reprod. 1998 Sep;3(3):155–163. doi: 10.1530/ror.0.0030155. [DOI] [PubMed] [Google Scholar]
  47. Zhang J., Wang C. W., Krey L., Liu H., Meng L., Blaszczyk A., Adler A., Grifo J. In vitro maturation of human preovulatory oocytes reconstructed by germinal vesicle transfer. Fertil Steril. 1999 Apr;71(4):726–731. doi: 10.1016/s0015-0282(98)00549-4. [DOI] [PubMed] [Google Scholar]

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