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. 2001 Oct;199(Pt 4):429–434. doi: 10.1046/j.1469-7580.2001.19940429.x

Comparing human skeletal muscle architectural parameters of cadavers with in vivo ultrasonographic measurements

D C MARTIN 1 ,✉, M K MEDRI 1 , R S CHOW 1 , V OXORN 3 , R N LEEKAM 2 , A M AGUR 1 , N H McKEE 1
PMCID: PMC1468353  PMID: 11693303

Abstract

The purpose of this study was to document and compare the architectural parameters (fibre bundle length, angle of pennation) of human skeletal muscle in cadaveric specimens and live subjects. The medial (MG) and lateral (LG) gastrocnemius, and posterior (PS) and anterior (AS) soleus were examined bilaterally in 5 cadavers (mean age 72.6, range 65–83 y) and 9 live subjects (mean age 76.3, range 70–92 y). Data were obtained from direct measurement of cadaveric specimens and from ultrasonographic scans of the live subjects. In cadaveric muscle, fibre bundles were isolated; their length was measured in millimetres and pennation angles were recorded in degrees. In live muscle, similar measurements were taken from ultrasonographic scans of relaxed and contracted muscle. For the scans of relaxed muscle, subjects were positioned prone with the foot at a 90° angle to the leg, and for scans of contracted muscle, subjects were asked to sustain full plantarflexion during the scanning process. Fibre bundle length and angle of pennation were compared at matched locations in both groups. It was found that the relationship between cadaveric and in vivo values for fibre length and angle of pennation varied between muscle parts. The cadaveric architectural parameters did not tend to lie consistently towards either extreme of relaxation or contraction. Rather, within MG, PS and AS, cadaveric fibre bundle lengths lay between those for relaxed and contracted in vivo muscle. Similarly both the anterior and posterior cadaveric fibre angles of pennation lay between the in vivo values within LG and PS. In summary, architectural characteristics of cadaveric muscle differ from both relaxed and contracted in vivo muscle. Therefore, when developing models of skeletal muscle based on cadaveric studies, the architectural differences between live and cadaveric tissue should be taken into consideration.

Keywords: Fibre length, pennation angle, gastrocnemius, soleus, ultrasound

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Selected References

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  1. BENDALL J. R. The shortening of rabbit muscles during rigor mortis; its relation to the breakdown of adenosine triphosphate and creatine phosphate and to muscular contraction. J Physiol. 1951 Jun;114(1-2):71–88. doi: 10.1113/jphysiol.1951.sp004604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chow R. S., Medri M. K., Martin D. C., Leekam R. N., Agur A. M., McKee N. H. Sonographic studies of human soleus and gastrocnemius muscle architecture: gender variability. Eur J Appl Physiol. 2000 Jun;82(3):236–244. doi: 10.1007/s004210050677. [DOI] [PubMed] [Google Scholar]
  3. Cutts A. Shrinkage of muscle fibres during the fixation of cadaveric tissue. J Anat. 1988 Oct;160:75–78. [PMC free article] [PubMed] [Google Scholar]
  4. Cutts A. The range of sarcomere lengths in the muscles of the human lower limb. J Anat. 1988 Oct;160:79–88. [PMC free article] [PubMed] [Google Scholar]
  5. DAVIES R. E. A MOLECULAR THEORY OF MUSCLE CONTRACTION: CALCIUM-DEPENDENT CONTRACTIONS WITH HYDROGEN BOND FORMATION PLUS ATP-DEPENDENT EXTENSIONS OF PART OF THE MYOSIN-ACTIN CROSS-BRIDGES. Nature. 1963 Sep 14;199:1068–1074. doi: 10.1038/1991068a0. [DOI] [PubMed] [Google Scholar]
  6. Fenster A., Downey D. B. Three-dimensional ultrasound imaging. Annu Rev Biomed Eng. 2000;2:457–475. doi: 10.1146/annurev.bioeng.2.1.457. [DOI] [PubMed] [Google Scholar]
  7. Friederich J. A., Brand R. A. Muscle fiber architecture in the human lower limb. J Biomech. 1990;23(1):91–95. doi: 10.1016/0021-9290(90)90373-b. [DOI] [PubMed] [Google Scholar]
  8. Fukunaga T., Ichinose Y., Ito M., Kawakami Y., Fukashiro S. Determination of fascicle length and pennation in a contracting human muscle in vivo. J Appl Physiol (1985) 1997 Jan;82(1):354–358. doi: 10.1152/jappl.1997.82.1.354. [DOI] [PubMed] [Google Scholar]
  9. Fukunaga T., Kawakami Y., Kuno S., Funato K., Fukashiro S. Muscle architecture and function in humans. J Biomech. 1997 May;30(5):457–463. doi: 10.1016/s0021-9290(96)00171-6. [DOI] [PubMed] [Google Scholar]
  10. Herbert R. D., Gandevia S. C. Changes in pennation with joint angle and muscle torque: in vivo measurements in human brachialis muscle. J Physiol. 1995 Apr 15;484(Pt 2):523–532. doi: 10.1113/jphysiol.1995.sp020683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hooper A. C., Hegarty P. V. The percentage of passively contracted fibres in rigor skeletal muscles from different species. Comp Biochem Physiol A Comp Physiol. 1973 Jun 1;45(2):411–416. doi: 10.1016/0300-9629(73)90447-7. [DOI] [PubMed] [Google Scholar]
  12. Kawakami Y., Ichinose Y., Fukunaga T. Architectural and functional features of human triceps surae muscles during contraction. J Appl Physiol (1985) 1998 Aug;85(2):398–404. doi: 10.1152/jappl.1998.85.2.398. [DOI] [PubMed] [Google Scholar]
  13. LOCKER R. H. Striation patterns of ox muscle in rigor mortis. J Biophys Biochem Cytol. 1959 Dec;6:419–422. doi: 10.1083/jcb.6.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leekam R. N., Agur A. M., McKee N. H. Using sonography to diagnose injury of plantaris muscles and tendons. AJR Am J Roentgenol. 1999 Jan;172(1):185–189. doi: 10.2214/ajr.172.1.9888765. [DOI] [PubMed] [Google Scholar]
  15. Legreneur P., Morlon B., Van Hoecke J. Simulation of in situ soleus isometric force output as a function of neural excitation. J Biomech. 1996 Nov;29(11):1455–1462. doi: 10.1016/0021-9290(96)84541-6. [DOI] [PubMed] [Google Scholar]
  16. Maganaris C. N., Baltzopoulos V., Sargeant A. J. In vivo measurements of the triceps surae complex architecture in man: implications for muscle function. J Physiol. 1998 Oct 15;512(Pt 2):603–614. doi: 10.1111/j.1469-7793.1998.603be.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Narici M. Human skeletal muscle architecture studied in vivo by non-invasive imaging techniques: functional significance and applications. J Electromyogr Kinesiol. 1999 Apr;9(2):97–103. doi: 10.1016/s1050-6411(98)00041-8. [DOI] [PubMed] [Google Scholar]
  18. Oxorn V. M., Agur A. M., McKee N. H. Resolving discrepancies in image research: the importance of direct observation in the illustration of the human soleus muscle. J Biocommun. 1998;25(1):16–26. [PubMed] [Google Scholar]
  19. Rankin R. N., Fenster A., Downey D. B., Munk P. L., Levin M. F., Vellet A. D. Three-dimensional sonographic reconstruction: techniques and diagnostic applications. AJR Am J Roentgenol. 1993 Oct;161(4):695–702. doi: 10.2214/ajr.161.4.8372741. [DOI] [PubMed] [Google Scholar]
  20. Roberts N. Review of SMR Workshop on MRI and MRS of muscle. Magn Reson Med. 1995 Jul;34(1):1–3. doi: 10.1002/mrm.1910340102. [DOI] [PubMed] [Google Scholar]
  21. Rutherford O. M., Jones D. A. Measurement of fibre pennation using ultrasound in the human quadriceps in vivo. Eur J Appl Physiol Occup Physiol. 1992;65(5):433–437. doi: 10.1007/BF00243510. [DOI] [PubMed] [Google Scholar]
  22. Scott S. H., Engstrom C. M., Loeb G. E. Morphometry of human thigh muscles. Determination of fascicle architecture by magnetic resonance imaging. J Anat. 1993 Apr;182(Pt 2):249–257. [PMC free article] [PubMed] [Google Scholar]
  23. Spoor C. W., van Leeuwen J. L., van der Meulen W. J., Huson A. Active force-length relationship of human lower-leg muscles estimated from morphological data: a comparison of geometric muscle models. Eur J Morphol. 1991;29(3):137–160. [PubMed] [Google Scholar]
  24. Thorpe S. K., Li Y., Crompton R. H., Alexander R. M. Stresses in human leg muscles in running and jumping determined by force plate analysis and from published magnetic resonance images. J Exp Biol. 1998 Jan;201(Pt 1):63–70. doi: 10.1242/jeb.201.1.63. [DOI] [PubMed] [Google Scholar]
  25. Wickiewicz T. L., Roy R. R., Powell P. L., Edgerton V. R. Muscle architecture of the human lower limb. Clin Orthop Relat Res. 1983 Oct;(179):275–283. [PubMed] [Google Scholar]

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