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. 2001 Nov;199(Pt 5):539–545. doi: 10.1046/j.1469-7580.2001.19950539.x

Fibrocartilage at the entheses of the suprascapular (superior transverse scapular) ligament of man—a ligament spanning two regions of a single bone

B MORIGGL 1 ,✉, P JAX 2 , S MILZ 1 , A BÜTTNER 3 , M BENJAMIN 4
PMCID: PMC1468365  PMID: 11760885

Abstract

The suprascapular ligament converts the suprascapular notch into a foramen separating the vessels and nerve of the same name. It connects 2 regions of the same bone and does not cross any joint, and no mechanical function has yet been attributed to it. Nevertheless, variations in its thickness and length, and its tendency to ossify, suggest that the ligament responds to changes in mechanical load. This should be reflected in the composition of the extracellular matrix. The primary purpose of the present study is to demonstrate that the suprascapular ligament has fibrocartilaginous entheses (i.e. insertion sites), even though there is no obvious change in insertional angle that directly results from joint movement. Such a change is more typical of tendons or ligaments that cross highly mobile joints. The complete ligament (including both entheses) was removed from 7 cadavers shortly after death and fixed in 90% methanol. Cryosections were immunolabelled with a panel of monoclonal antibodies against collagens (types I, II, III, VI), glycosaminoglycans (chondroitin 4 sulphate, chondroitin 6 sulphate, dermatan sulphate and keratan sulphates), proteoglycans (aggrecan and versican) and link protein. Both entheses were strongly fibrocartilaginous, and a moderately fibrocartilaginous matrix was also detected throughout the remainder of the ligament. The extracellular matrix of both entheses labelled strongly for type II collagen, aggrecan and link protein. The fibrocartilaginous character of the entheses suggests that the insertion sites of the ligament are subject to both compressive and tensile loading and are regions of stress concentration. This in turn probably reflects the complex shape of the scapula and the presence of a conspicuous indentation (the suprascapular notch) near the ligament. The loading patterns may reflect either the attachment of muscles and/or the forces transmitted to the suprascapular ligament from the neighbouring coracoclavicular ligament.

Keywords: Fibrocartilage, type II collagen, glycosaminoglycans, proteoglycans, aggrecan

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Selected References

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  1. Arnold G. Festigkeit und Kraft-Längenäderungs-Verhalten der Strecksehnen des menschlichen fusses. Res Exp Med (Berl) 1974;164(2):123–136. doi: 10.1007/BF01851653. [DOI] [PubMed] [Google Scholar]
  2. Asher R. A., Scheibe R. J., Keiser H. D., Bignami A. On the existence of a cartilage-like proteoglycan and link proteins in the central nervous system. Glia. 1995 Apr;13(4):294–308. doi: 10.1002/glia.440130406. [DOI] [PubMed] [Google Scholar]
  3. Asher R., Perides G., Vanderhaeghen J. J., Bignami A. Extracellular matrix of central nervous system white matter: demonstration of an hyaluronate-protein complex. J Neurosci Res. 1991 Mar;28(3):410–421. doi: 10.1002/jnr.490280314. [DOI] [PubMed] [Google Scholar]
  4. Benjamin M., Evans E. J., Copp L. The histology of tendon attachments to bone in man. J Anat. 1986 Dec;149:89–100. [PMC free article] [PubMed] [Google Scholar]
  5. Benjamin M., Ralphs J. R. Fibrocartilage in tendons and ligaments--an adaptation to compressive load. J Anat. 1998 Nov;193(Pt 4):481–494. doi: 10.1046/j.1469-7580.1998.19340481.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooper R. R., Misol S. Tendon and ligament insertion. A light and electron microscopic study. J Bone Joint Surg Am. 1970 Jan;52(1):1–20. [PubMed] [Google Scholar]
  7. Evans E. J., Benjamin M., Pemberton D. J. Fibrocartilage in the attachment zones of the quadriceps tendon and patellar ligament of man. J Anat. 1990 Aug;171:155–162. [PMC free article] [PubMed] [Google Scholar]
  8. GARDNER E., GRAY D. J. Prenatal development of the human shoulder and acromioclavicular joints. Am J Anat. 1953 Mar;92(2):219–276. doi: 10.1002/aja.1000920203. [DOI] [PubMed] [Google Scholar]
  9. Hayashi K., Ishidou Y., Yonemori K., Nagamine T., Origuchi N., Maeda S., Imamura T., Kato M., Yoshida H., Sampath T. K. Expression and localization of bone morphogenetic proteins (BMPs) and BMP receptors in ossification of the ligamentum flavum. Bone. 1997 Jul;21(1):23–30. doi: 10.1016/s8756-3282(97)00080-x. [DOI] [PubMed] [Google Scholar]
  10. Hessle H., Engvall E. Type VI collagen. Studies on its localization, structure, and biosynthetic form with monoclonal antibodies. J Biol Chem. 1984 Mar 25;259(6):3955–3961. [PubMed] [Google Scholar]
  11. Holmdahl R., Rubin K., Klareskog L., Larsson E., Wigzell H. Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies. Arthritis Rheum. 1986 Mar;29(3):400–410. doi: 10.1002/art.1780290314. [DOI] [PubMed] [Google Scholar]
  12. Hoshi K., Amizuka N., Sakou T., Kurokawa T., Ozawa H. Fibroblasts of spinal ligaments pathologically differentiate into chondrocytes induced by recombinant human bone morphogenetic protein-2: morphological examinations for ossification of spinal ligaments. Bone. 1997 Aug;21(2):155–162. doi: 10.1016/s8756-3282(97)00106-3. [DOI] [PubMed] [Google Scholar]
  13. KNESE K. H., BIERMANN H. Die Knochenbildung an Sehnen- und Bandansătzen im Bereich ursprünglich chondraier Apophysen. Z Zellforsch Mikrosk Anat. 1958;49(2):142–187. [PubMed] [Google Scholar]
  14. Loren G. J., Lieber R. L. Tendon biomechanical properties enhance human wrist muscle specialization. J Biomech. 1995 Jul;28(7):791–799. doi: 10.1016/0021-9290(94)00137-s. [DOI] [PubMed] [Google Scholar]
  15. Milz S., Valassis G., Büttner A., Maier M., Putz R., Ralphs J. R., Benjamin M. Fibrocartilage in the transverse ligament of the human acetabulum. J Anat. 2001 Feb;198(Pt 2):223–228. doi: 10.1046/j.1469-7580.2001.19820223.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moriggl B. Grundlagen, Möglichkeiten und Grenzen der Sonographie osteofibröser Kanäle im Schulterbereich. Teil 1. Ann Anat. 1997 Aug;179(4):355–373. [PubMed] [Google Scholar]
  17. Moriggl B. Grundlagen, Möglichkeiten und Grenzen der Sonographie osteofibröser Kanäle im Schulterbereich. Teil 2. Ann Anat. 1997 Aug;179(4):375–392. [PubMed] [Google Scholar]
  18. Rufai A., Ralphs J. R., Benjamin M. Structure and histopathology of the insertional region of the human Achilles tendon. J Orthop Res. 1995 Jul;13(4):585–593. doi: 10.1002/jor.1100130414. [DOI] [PubMed] [Google Scholar]
  19. SCAPINELLI R. SESAMOID BONES IN THE LIGAMENTUM NUCHAE OF MAN. J Anat. 1963 Jul;97:417–422. [PMC free article] [PubMed] [Google Scholar]
  20. SCHNEIDER H. Zur Struktur der Sehnenansatzzonen. Z Anat Entwicklungsgesch. 1956;119(5):431–456. [PubMed] [Google Scholar]
  21. Shaibani A., Workman R., Rothschild B. M. The significance of enthesopathy as a skeletal phenomenon. Clin Exp Rheumatol. 1993 Jul-Aug;11(4):399–403. [PubMed] [Google Scholar]
  22. Yonemori K., Imamura T., Ishidou Y., Okano T., Matsunaga S., Yoshida H., Kato M., Sampath T. K., Miyazono K., ten Dijke P. Bone morphogenetic protein receptors and activin receptors are highly expressed in ossified ligament tissues of patients with ossification of the posterior longitudinal ligament. Am J Pathol. 1997 Apr;150(4):1335–1347. [PMC free article] [PubMed] [Google Scholar]

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