Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1997 Sep 1;25(17):3486–3489. doi: 10.1093/nar/25.17.3486

The binding of two dimers of IciA protein to the dnaA promoter 1P element enhances the binding of RNA polymerase to the dnaA promoter 1P.

Y Lee 1, H Lee 1, J Yim 1, D Hwang 1
PMCID: PMC146913  PMID: 9254708

Abstract

Transcription of the dnaA gene from the promoter 1P has been shown to be activated in vitro and in vivo by the binding of IciA protein to two sites on the dnaA promoter region [Lee, Y. S., Kim, H., and Hwang, D. S. (1996) Mol. Microbiol . 19, 389-396; Lee, Y. S., and Hwang, D. S. (1997) J. Biol. Chem. 272, 83-88]. In vitro transcription assays using DNA fragments carrying variable combinations of two IciA binding sites revealed that IciA binding site I (IciA I site), which is located upstream of the promoter 1P, is responsible for the transcriptional activation. Binding of one dimeric IciA protein to the IciA I site is followed by binding of the second dimer. Two dimers of IciA protein, rather than one dimer, on the IciA I site appeared to enhance the binding of RNA polymerase to the promoter 1P, resulting in the activation of transcription from the promoter 1P.

Full Text

The Full Text of this article is available as a PDF (99.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atlung T., Clausen E. S., Hansen F. G. Autoregulation of the dnaA gene of Escherichia coli K12. Mol Gen Genet. 1985;200(3):442–450. doi: 10.1007/BF00425729. [DOI] [PubMed] [Google Scholar]
  2. Atlung T., Løbner-Olesen A., Hansen F. G. Overproduction of DnaA protein stimulates initiation of chromosome and minichromosome replication in Escherichia coli. Mol Gen Genet. 1987 Jan;206(1):51–59. doi: 10.1007/BF00326535. [DOI] [PubMed] [Google Scholar]
  3. Bramhill D., Kornberg A. A model for initiation at origins of DNA replication. Cell. 1988 Sep 23;54(7):915–918. doi: 10.1016/0092-8674(88)90102-x. [DOI] [PubMed] [Google Scholar]
  4. Bramhill D., Kornberg A. Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome. Cell. 1988 Mar 11;52(5):743–755. doi: 10.1016/0092-8674(88)90412-6. [DOI] [PubMed] [Google Scholar]
  5. Braun R. E., O'Day K., Wright A. Autoregulation of the DNA replication gene dnaA in E. coli K-12. Cell. 1985 Jan;40(1):159–169. doi: 10.1016/0092-8674(85)90319-8. [DOI] [PubMed] [Google Scholar]
  6. Fisher R. F., Long S. R. Interactions of NodD at the nod Box: NodD binds to two distinct sites on the same face of the helix and induces a bend in the DNA. J Mol Biol. 1993 Oct 5;233(3):336–348. doi: 10.1006/jmbi.1993.1515. [DOI] [PubMed] [Google Scholar]
  7. Hansen E. B., Hansen F. G., von Meyenburg K. The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12. Nucleic Acids Res. 1982 Nov 25;10(22):7373–7385. doi: 10.1093/nar/10.22.7373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hansen F. G., Hansen E. B., Atlung T. The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli. EMBO J. 1982;1(9):1043–1048. doi: 10.1002/j.1460-2075.1982.tb01294.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hansen F. G., Koefoed S., Sørensen L., Atlung T. Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli. EMBO J. 1987 Jan;6(1):255–258. doi: 10.1002/j.1460-2075.1987.tb04747.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hryniewicz M. M., Kredich N. M. Stoichiometry of binding of CysB to the cysJIH, cysK, and cysP promoter regions of Salmonella typhimurium. J Bacteriol. 1994 Jun;176(12):3673–3682. doi: 10.1128/jb.176.12.3673-3682.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hwang D. S., Kornberg A. A novel protein binds a key origin sequence to block replication of an E. coli minichromosome. Cell. 1990 Oct 19;63(2):325–331. doi: 10.1016/0092-8674(90)90165-b. [DOI] [PubMed] [Google Scholar]
  12. Hwang D. S., Thöny B., Kornberg A. IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication. J Biol Chem. 1992 Feb 5;267(4):2209–2213. [PubMed] [Google Scholar]
  13. Kücherer C., Lother H., Kölling R., Schauzu M. A., Messer W. Regulation of transcription of the chromosomal dnaA gene of Escherichia coli. Mol Gen Genet. 1986 Oct;205(1):115–121. doi: 10.1007/BF02428040. [DOI] [PubMed] [Google Scholar]
  14. Lee Y. S., Hwang D. S. Occlusion of RNA polymerase by oligomerization of DnaA protein over the dnaA promoter of Escherichia coli. J Biol Chem. 1997 Jan 3;272(1):83–88. [PubMed] [Google Scholar]
  15. Lee Y. S., Kim H., Hwang D. S. Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli. Mol Microbiol. 1996 Jan;19(2):389–396. doi: 10.1046/j.1365-2958.1996.485902.x. [DOI] [PubMed] [Google Scholar]
  16. Løbner-Olesen A., Skarstad K., Hansen F. G., von Meyenburg K., Boye E. The DnaA protein determines the initiation mass of Escherichia coli K-12. Cell. 1989 Jun 2;57(5):881–889. doi: 10.1016/0092-8674(89)90802-7. [DOI] [PubMed] [Google Scholar]
  17. Parsek M. R., Kivisaar M., Chakrabarty A. M. Differential DNA bending introduced by the Pseudomonas putida LysR-type regulator, CatR, at the plasmid-borne pheBA and chromosomal catBC promoters. Mol Microbiol. 1995 Mar;15(5):819–828. doi: 10.1111/j.1365-2958.1995.tb02352.x. [DOI] [PubMed] [Google Scholar]
  18. Skarstad K., Løbner-Olesen A., Atlung T., von Meyenburg K., Boye E. Initiation of DNA replication in Escherichia coli after overproduction of the DnaA protein. Mol Gen Genet. 1989 Jul;218(1):50–56. doi: 10.1007/BF00330564. [DOI] [PubMed] [Google Scholar]
  19. Thöny B., Hwang D. S., Fradkin L., Kornberg A. iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4066–4070. doi: 10.1073/pnas.88.10.4066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wang L., Helmann J. D., Winans S. C. The A. tumefaciens transcriptional activator OccR causes a bend at a target promoter, which is partially relaxed by a plant tumor metabolite. Cell. 1992 May 15;69(4):659–667. doi: 10.1016/0092-8674(92)90229-6. [DOI] [PubMed] [Google Scholar]
  21. Wang Q. P., Kaguni J. M. Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Mol Gen Genet. 1987 Oct;209(3):518–525. doi: 10.1007/BF00331158. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES