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. 1996 Jul;104(7):774–778. doi: 10.1289/ehp.96104774

Alterations of cytochrome P450-dependent monooxygenase activities in Eriocheir japonicus in response to water pollution.

M Ishizuka 1, H Hoshi 1, N Minamoto 1, M Masuda 1, A Kazusaka 1, S Fujita 1
PMCID: PMC1469413  PMID: 8841764

Abstract

Eriocheir japonicus, fresh-water crabs inhabiting rivers and estuaries in Japan, were investigated for cytochrome P450 (CYP)-dependent drug-metabolizing enzyme activities to see if these activities reflect the river pollution gradient. From the laboratory dose-response experiments, we found that the polycyclic aromatic hydrocarbon (PAH) 3-methylcholanthrene induced total CYP contents, ethoxycoumarin O-deethylase activity, and bunitrolol 4-hydroxylase activity in crab hepatopancreas. In the field studies, crabs collected from the river with the highest concentration of PAHs exhibited the highest levels of CYP, the highest activities of benzo[a]pyrene 3-hydroxylase, imipramine 2-hydroxylase, bunitrolol 4-hydroxylase, ethoxycoumarin O-deethylase, and the ability to metabolically activate benzo[a]pyrene, but erythromycin N-demethylase activity was not induced. The correlation between PAH levels and drug-metabolizing enzyme activities in female crabs were not as marked as in male crabs. The levels and activities of CYP did not appear to reflect the concentrations of organochlorines and polychlorinated biphenyl congeners (PCBs) studied in the fat of crab hepatopancreas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames B. N., Mccann J., Yamasaki E. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res. 1975 Dec;31(6):347–364. doi: 10.1016/0165-1161(75)90046-1. [DOI] [PubMed] [Google Scholar]
  2. Batel R., Bihari N., Zahn R. K. 3-Methylcholanthrene does induce mixed function oxidase activity in hepatopancreas of spiny crab Maja crispata. Comp Biochem Physiol C. 1988;90(2):435–438. doi: 10.1016/0742-8413(88)90023-0. [DOI] [PubMed] [Google Scholar]
  3. Bonfils C., Dalet C., Dalet-Beluche I., Maurel P. Cytochrome P-450 isozyme LM3b from rabbit liver microsomes. Induction by triacetyloleandomycin purification and characterization. J Biol Chem. 1983 May 10;258(9):5358–5362. [PubMed] [Google Scholar]
  4. Chiba M., Nishihara E., Fujita S., Suzuki T. Position selective sex difference in imipramine metabolism in rat liver microsomes. Biochem Pharmacol. 1985 Mar 15;34(6):898–900. doi: 10.1016/0006-2952(85)90773-7. [DOI] [PubMed] [Google Scholar]
  5. Goksøyr A., Husøy A. M., Larsen H. E., Klungsøyr J., Wilhelmsen S., Maage A., Brevik E. M., Andersson T., Celander M., Pesonen M. Environmental contaminants and biochemical responses in flatfish from the Hvaler Archipelago in Norway. Arch Environ Contam Toxicol. 1991 Nov;21(4):486–496. doi: 10.1007/BF01183869. [DOI] [PubMed] [Google Scholar]
  6. Greenlee W. F., Poland A. An improved assay of 7-ethoxycoumarin O-deethylase activity: induction of hepatic enzyme activity in C57BL/6J and DBA/2J mice by phenobarbital, 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin. J Pharmacol Exp Ther. 1978 Jun;205(3):596–605. [PubMed] [Google Scholar]
  7. Haasch M. L., Graf W. K., Quardokus E. M., Mayer R. T., Lech J. J. Use of 7-alkoxyphenoxazones, 7-alkoxycoumarins and 7-alkoxyquinolines as fluorescent substrates for rainbow trout hepatic microsomes after treatment with various inducers. Biochem Pharmacol. 1994 Mar 2;47(5):893–903. doi: 10.1016/0006-2952(94)90490-1. [DOI] [PubMed] [Google Scholar]
  8. Hayatsu H., Oka T., Wakata A., Ohara Y., Hayatsu T., Kobayashi H., Arimoto S. Adsorption of mutagens to cotton bearing covalently bound trisulfo-copper-phthalocyanine. Mutat Res. 1983 Mar;119(3):233–238. doi: 10.1016/0165-7992(83)90166-5. [DOI] [PubMed] [Google Scholar]
  9. Ishida R., Fujita S., Suzuki T. Bunitrolol metabolism and its inhibition by cimetidine. J Pharm Pharmacol. 1988 Jan;40(1):64–65. doi: 10.1111/j.2042-7158.1988.tb05155.x. [DOI] [PubMed] [Google Scholar]
  10. James M. O. Cytochrome P450 monooxygenases in crustaceans. Xenobiotica. 1989 Oct;19(10):1063–1076. doi: 10.3109/00498258909043162. [DOI] [PubMed] [Google Scholar]
  11. Kannan K., Tanabe S., Quynh H. T., Hue N. D., Tatsukawa R. Residue pattern and dietary intake of persistent organochlorine compounds in foodstuffs from Vietnam. Arch Environ Contam Toxicol. 1992 May;22(4):367–374. doi: 10.1007/BF00212555. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. McFarland V. A., Clarke J. U. Environmental occurrence, abundance, and potential toxicity of polychlorinated biphenyl congeners: considerations for a congener-specific analysis. Environ Health Perspect. 1989 May;81:225–239. doi: 10.1289/ehp.8981225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murk A., Morse D., Boon J., Brouwer A. In vitro metabolism of 3,3',4,4'-tetrachlorobiphenyl in relation to ethoxyresorufin-O-deethylase activity in liver microsomes of some wildlife species and rat. Eur J Pharmacol. 1994 Apr 4;270(2-3):253–261. doi: 10.1016/0926-6917(94)90069-8. [DOI] [PubMed] [Google Scholar]
  15. Nebert D. W., Gelboin H. V. Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. I. Assay and properties of induced enzyme. J Biol Chem. 1968 Dec 10;243(23):6242–6249. [PubMed] [Google Scholar]
  16. OMURA T., SATO R. THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. J Biol Chem. 1964 Jul;239:2370–2378. [PubMed] [Google Scholar]
  17. Pohl R. J., Bend J. R., Guarino A. M., Fouts J. R. Hepatic microsomal mixed-function oxidase activity of several marine species from coastal Maine. Drug Metab Dispos. 1974 Nov-Dec;2(6):545–555. [PubMed] [Google Scholar]
  18. Porte C., Solé M., Albaigés J., Livingstone D. R. Responses of mixed-function oxygenase and antioxidase enzyme system of Mytilus sp. to organic pollution. Comp Biochem Physiol C. 1991;100(1-2):183–186. doi: 10.1016/0742-8413(91)90150-r. [DOI] [PubMed] [Google Scholar]
  19. Schlenk D., Ronis M. J., Miranda C. L., Buhler D. R. Channel catfish liver monooxygenases. Immunological characterization of constitutive cytochromes P450 and the absence of active flavin-containing monooxygenases. Biochem Pharmacol. 1993 Jan 7;45(1):217–221. doi: 10.1016/0006-2952(93)90395-d. [DOI] [PubMed] [Google Scholar]
  20. Stegeman J. J., Lech J. J. Cytochrome P-450 monooxygenase systems in aquatic species: carcinogen metabolism and biomarkers for carcinogen and pollutant exposure. Environ Health Perspect. 1991 Jan;90:101–109. doi: 10.1289/ehp.90-1519513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Uemura T., Chiesara E. NADH-dependent aryl hydrocarbon hydroxylase in rat liver mitochondrial outer membrane. Eur J Biochem. 1976 Jul 1;66(2):293–307. doi: 10.1111/j.1432-1033.1976.tb10519.x. [DOI] [PubMed] [Google Scholar]

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