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. 1997 Sep 15;25(18):3747–3748. doi: 10.1093/nar/25.18.3747

Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

Y Kinoshita 1, K Nishigaki 1, Y Husimi 1
PMCID: PMC146945  PMID: 9278501

Abstract

A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.

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Selected References

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