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Nucleic Acids Research logoLink to Nucleic Acids Research
. 1997 Sep 15;25(18):3749–3750. doi: 10.1093/nar/25.18.3749

Multiplex genotyping of PCR products with MassTag-labeled primers.

L A Haff 1, I P Smirnov 1
PMCID: PMC146949  PMID: 9278502

Abstract

A simple mass spectrometric based assay, the PinPoint assay, has previously been described for typing single nucleotide polymorphisms. The identity of a polymorphism is determined by mass differences of single base extended genotyping primers as determined by MALDI-TOF mass spectrometry. A simple method for multiplexing the assay is described, employing multiple primers with 5'oligo(dT) sequences (MassTags) which serve to mass discriminate the peaks of multiple extended and non-extended primers. The assay is extremely rapid and requires no labeling reagents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Haff L. A., Smirnov I. P. Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry. Genome Res. 1997 Apr;7(4):378–388. doi: 10.1101/gr.7.4.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Miki Y., Swensen J., Shattuck-Eidens D., Futreal P. A., Harshman K., Tavtigian S., Liu Q., Cochran C., Bennett L. M., Ding W. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science. 1994 Oct 7;266(5182):66–71. doi: 10.1126/science.7545954. [DOI] [PubMed] [Google Scholar]
  3. Nikiforov T. T., Rendle R. B., Goelet P., Rogers Y. H., Kotewicz M. L., Anderson S., Trainor G. L., Knapp M. R. Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms. Nucleic Acids Res. 1994 Oct 11;22(20):4167–4175. doi: 10.1093/nar/22.20.4167. [DOI] [PMC free article] [PubMed] [Google Scholar]

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