Abstract
Wet membranes in DNA hybridisation procedures can be very difficult to handle. This adds considerable time to the length of experiments and is a serious obstacle to the performance of large-scale investigations. A method is described to give rigidity to membranes, thereby making them easier to handle and in turn improving both the speed and efficiency of experimental work.
Full Text
The Full Text of this article is available as a PDF (26.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ioannou P. A., Amemiya C. T., Garnes J., Kroisel P. M., Shizuya H., Chen C., Batzer M. A., de Jong P. J. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nat Genet. 1994 Jan;6(1):84–89. doi: 10.1038/ng0194-84. [DOI] [PubMed] [Google Scholar]
- Maier E., Roest Crollius H., Lehrach H. Hybridisation techniques on gridded high density DNA and in situ colony filters based on fluorescence detection. Nucleic Acids Res. 1994 Aug 25;22(16):3423–3424. doi: 10.1093/nar/22.16.3423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCarthy L., Hunter K., Schalkwyk L., Riba L., Anson S., Mott R., Newell W., Bruley C., Bar I., Ramu E. Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5302–5306. doi: 10.1073/pnas.92.12.5302. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meier-Ewert S., Maier E., Ahmadi A., Curtis J., Lehrach H. An automated approach to generating expressed sequence catalogues. Nature. 1993 Jan 28;361(6410):375–376. doi: 10.1038/361375a0. [DOI] [PubMed] [Google Scholar]
- Nizetić D., Gellen L., Hamvas R. M., Mott R., Grigoriev A., Vatcheva R., Zehetner G., Yaspo M. L., Dutriaux A., Lopes C. An integrated YAC-overlap and 'cosmid-pocket' map of the human chromosome 21. Hum Mol Genet. 1994 May;3(5):759–770. doi: 10.1093/hmg/3.5.759. [DOI] [PubMed] [Google Scholar]