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. 1997 Jun;105(Suppl 4):801–806. doi: 10.1289/ehp.97105s4801

Biomarkers of human exposure to pesticides.

W A Anwar 1
PMCID: PMC1470029  PMID: 9255564

Abstract

For centuries, several hundred pesticides have been used to control insects. These pesticides differ greatly in their mode of action, uptake by the body, metabolism, elimination from the body, and toxicity to humans. Potential exposure from the environment can be estimated by environmental monitoring. Actual exposure (uptake) is measured by the biological monitoring of human tissues and body fluids. Biomarkers are used to detect the effects of pesticides before adverse clinical health effects occur. Pesticides and their metabolites are measured in biological samples, serum, fat, urine, blood, or breast milk by the usual analytical techniques. Biochemical responses to environmental chemicals provide a measure of toxic effect. A widely used biochemical biomarker, cholinesterase depression, measures exposure to organophosphorus insecticides. Techniques that measure DNA damage (e.g., detection of DNA adducts) provide a powerful tool in measuring environmental effects. Adducts to hemoglobin have been detected with several pesticides. Determination of chromosomal aberration rates in cultured lymphocytes is an established method of monitoring populations occupationally or environmentally exposed to known or suspected mutagenic-carcinogenic agents. There are several studies on the cytogenetic effects of work with pesticide formulations. The majority of these studies report increases in the frequency of chromosomal aberrations and/or sister chromatid exchanges among the exposed workers. Biomarkers will have a major impact on the study of environmental risk factors. The basic aim of scientists exploring these issues is to determine the nature and consequences of genetic change or variation, with the ultimate purpose of predicting or preventing disease.

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Selected References

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  1. Ahlborg U. G., Lindgren J. E., Mercier M. Metabolism of pentachlorophenol. Arch Toxicol. 1974;32(4):271–281. doi: 10.1007/BF00330109. [DOI] [PubMed] [Google Scholar]
  2. Alavanja M. C., Blair A., Masters M. N. Cancer mortality in the U.S. flour industry. J Natl Cancer Inst. 1990 May 16;82(10):840–848. doi: 10.1093/jnci/82.10.840. [DOI] [PubMed] [Google Scholar]
  3. Ames R. G., Brown S. K., Mengle D. C., Kahn E., Stratton J. W., Jackson R. J. Cholinesterase activity depression among California agricultural pesticide applicators. Am J Ind Med. 1989;15(2):143–150. doi: 10.1002/ajim.4700150203. [DOI] [PubMed] [Google Scholar]
  4. Autrup H. Transplacental transfer of genotoxins and transplacental carcinogenesis. Environ Health Perspect. 1993 Jul;101 (Suppl 2):33–38. doi: 10.1289/ehp.93101s233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barthel E. Increased risk of lung cancer in pesticide-exposed male agricultural workers. J Toxicol Environ Health. 1981 Nov-Dec;8(5-6):1027–1040. doi: 10.1080/15287398109530135. [DOI] [PubMed] [Google Scholar]
  6. Basri Ustünbas H., Oztürk M. A., Hasanoğlu E., Doğan M. Organochlorine pesticide residues in human milk in Kayseri. Hum Exp Toxicol. 1994 May;13(5):299–302. doi: 10.1177/096032719401300501. [DOI] [PubMed] [Google Scholar]
  7. Blair A., Grauman D. J., Lubin J. H., Fraumeni J. F., Jr Lung cancer and other causes of death among licensed pesticide applicators. J Natl Cancer Inst. 1983 Jul;71(1):31–37. [PubMed] [Google Scholar]
  8. Blair D., Hoadley C., Hutson D. H. The distribution of dichlorvos in the tissues of mammals after its inhalation or intravenous administration. Toxicol Appl Pharmacol. 1975 Feb;31(2):243–253. doi: 10.1016/0041-008x(75)90160-x. [DOI] [PubMed] [Google Scholar]
  9. Brewster M. A., Hulka B. S., Lavy T. L. Biomarkers of pesticide exposure. Rev Environ Contam Toxicol. 1992;128:17–42. doi: 10.1007/978-1-4612-2964-3_2. [DOI] [PubMed] [Google Scholar]
  10. Burgess J. L., Bernstein J. N., Hurlbut K. Aldicarb poisoning. A case report with prolonged cholinesterase inhibition and improvement after pralidoxime therapy. Arch Intern Med. 1994 Jan 24;154(2):221–224. doi: 10.1001/archinte.154.2.221. [DOI] [PubMed] [Google Scholar]
  11. Cantor K. P., Blair A. Farming and mortality from multiple myeloma: a case-control study with the use of death certificates. J Natl Cancer Inst. 1984 Feb;72(2):251–255. [PubMed] [Google Scholar]
  12. Catenacci G., Maroni M., Cottica D., Pozzoli L. Assessment of human exposure to atrazine through the determination of free atrazine in urine. Bull Environ Contam Toxicol. 1990 Jan;44(1):1–7. doi: 10.1007/BF01702354. [DOI] [PubMed] [Google Scholar]
  13. Ciesielski S., Loomis D. P., Mims S. R., Auer A. Pesticide exposures, cholinesterase depression, and symptoms among North Carolina migrant farmworkers. Am J Public Health. 1994 Mar;84(3):446–451. doi: 10.2105/ajph.84.3.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Comer S. W., Staiff D. C., Armstrong J. F., Wolfe H. R. Exposure of workers to carbaryl. Bull Environ Contam Toxicol. 1975 Apr;13(4):385–391. doi: 10.1007/BF01721839. [DOI] [PubMed] [Google Scholar]
  15. Corrao G., Calleri M., Carle F., Russo R., Bosia S., Piccioni P. Cancer risk in a cohort of licensed pesticide users. Scand J Work Environ Health. 1989 Jun;15(3):203–209. doi: 10.5271/sjweh.1861. [DOI] [PubMed] [Google Scholar]
  16. Coye M. J., Lowe J. A., Maddy K. J. Biological monitoring of agricultural workers exposed to pesticides: II. Monitoring of intact pesticides and their metabolites. J Occup Med. 1986 Aug;28(8):628–636. doi: 10.1097/00043764-198608000-00019. [DOI] [PubMed] [Google Scholar]
  17. Curley A., Garrettson L. K. Acute chlordane poisoning. Clinical and chemical studies. Arch Environ Health. 1969 Feb;18(2):211–215. doi: 10.1080/00039896.1969.10665394. [DOI] [PubMed] [Google Scholar]
  18. D'Ercole A. J., Arthur R. D., Cain J. D., Barrentine B. F. Insecticide exposure of mothers and newborns in a rural agricultural area. Pediatrics. 1976 Jun;57(6):869–874. [PubMed] [Google Scholar]
  19. Das Y. T., Taskar P. K., Brown H. D., Chattopadhyay S. K. Exposure of professional pest control operator to dichlorvos (DDVP) and residue on house structures. Toxicol Lett. 1983 Jun;17(1-2):95–99. doi: 10.1016/0378-4274(83)90042-5. [DOI] [PubMed] [Google Scholar]
  20. Dulout F. N., Pastori M. C., Olivero O. A., González Cid M., Loria D., Matos E., Sobel N., de Bujan E. C., Albiano N. Sister-chromatid exchanges and chromosomal aberrations in a population exposed to pesticides. Mutat Res. 1985 Aug;143(4):237–244. doi: 10.1016/0165-7992(85)90087-9. [DOI] [PubMed] [Google Scholar]
  21. Edwards J. W., Priestly B. G. Effect of occupational exposure to aldrin on urinary D-glucaric acid, plasma dieldrin, and lymphocyte sister chromatid exchange. Int Arch Occup Environ Health. 1994;66(4):229–234. doi: 10.1007/BF00454360. [DOI] [PubMed] [Google Scholar]
  22. Forni A. Chromosomal aberrations in monitoring exposure to mutagens-carcinogens. IARC Sci Publ. 1984;(59):325–337. [PubMed] [Google Scholar]
  23. Fürst P., Fürst C., Wilmers K. Human milk as a bioindicator for body burden of PCDDs, PCDFs, organochlorine pesticides, and PCBs. Environ Health Perspect. 1994 Jan;102 (Suppl 1):187–193. doi: 10.1289/ehp.102-1566908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Garry V. F., Danzl T. J., Tarone R., Griffith J., Cervenka J., Krueger L., Whorton E. B., Jr, Nelson R. L. Chromosome rearrangements in fumigant appliers: possible relationship to non-Hodgkin's lymphoma risk. Cancer Epidemiol Biomarkers Prev. 1992 May-Jun;1(4):287–291. [PubMed] [Google Scholar]
  25. Garry V. F., Griffith J., Danzl T. J., Nelson R. L., Whorton E. B., Krueger L. A., Cervenka J. Human genotoxicity: pesticide applicators and phosphine. Science. 1989 Oct 13;246(4927):251–255. doi: 10.1126/science.2799386. [DOI] [PubMed] [Google Scholar]
  26. Garry V. F., Kelly J. T., Sprafka J. M., Edwards S., Griffith J. Survey of health and use characterization of pesticide appliers in Minnesota. Arch Environ Health. 1994 Sep-Oct;49(5):337–343. doi: 10.1080/00039896.1994.9954984. [DOI] [PubMed] [Google Scholar]
  27. Gudumak V. S., Popovich P. I., Nigulianu V. I., Marchenko V. P., Dontsu O. N., Tsaran O. A. Diagnosticheskoe znachenie issledovaniia aminokislotnogo obmena pri dlitel'nom kontakte s pestitsidami. Klin Lab Diagn. 1994;(3):17–19. [PubMed] [Google Scholar]
  28. Hayes A. L., Wise R. A., Weir F. W. Assessment of occupational exposure to organophosphates in pest control operators. Am Ind Hyg Assoc J. 1980 Aug;41(8):568–575. doi: 10.1080/15298668091425293. [DOI] [PubMed] [Google Scholar]
  29. He F., Wang S., Liu L., Chen S., Zhang Z., Sun J. Clinical manifestations and diagnosis of acute pyrethroid poisoning. Arch Toxicol. 1989;63(1):54–58. doi: 10.1007/BF00334635. [DOI] [PubMed] [Google Scholar]
  30. Johansen H. R., Becher G., Polder A., Skaare J. U. Congener-specific determination of polychlorinated biphenyls and organochlorine pesticides in human milk from Norwegian mothers living in Oslo. J Toxicol Environ Health. 1994 Jun;42(2):157–171. doi: 10.1080/15287399409531870. [DOI] [PubMed] [Google Scholar]
  31. Kreiss K., Zack M. M., Kimbrough R. D., Needham L. L., Smrek A. L., Jones B. T. Cross-sectional study of a community with exceptional exposure to DDT. JAMA. 1981 May 15;245(19):1926–1930. [PubMed] [Google Scholar]
  32. Larsen B. R., Turrio-Baldassarri L., Nilsson T., Iacovella N., Di Domenico A., Montagna M., Facchetti S. Toxic PCB congeners and organochlorine pesticides in Italian human milk. Ecotoxicol Environ Saf. 1994 Jun;28(1):1–13. doi: 10.1006/eesa.1994.1029. [DOI] [PubMed] [Google Scholar]
  33. Lee M. H., Ransdell J. F. A farmworker death due to pesticide toxicity: a case report. J Toxicol Environ Health. 1984;14(2-3):239–246. doi: 10.1080/15287398409530576. [DOI] [PubMed] [Google Scholar]
  34. MacMahon B., Monson R. R., Wang H. H., Zheng T. Z. A second follow-up of mortality in a cohort of pesticide applicators. J Occup Med. 1988 May;30(5):429–432. doi: 10.1097/00043764-198805000-00010. [DOI] [PubMed] [Google Scholar]
  35. McConnell R., Magnotti R. Screening for insecticide overexposure under field conditions: a reevaluation of the tintometric cholinesterase kit. Am J Public Health. 1994 Mar;84(3):479–481. doi: 10.2105/ajph.84.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Morgan D. P., Lin L. I., Saikaly H. H. Morbidity and mortality in workers occupationally exposed to pesticides. Arch Environ Contam Toxicol. 1980;9(3):349–382. doi: 10.1007/BF01057414. [DOI] [PubMed] [Google Scholar]
  37. Mortensen M. L. Management of acute childhood poisonings caused by selected insecticides and herbicides. Pediatr Clin North Am. 1986 Apr;33(2):421–445. doi: 10.1016/s0031-3955(16)35012-x. [DOI] [PubMed] [Google Scholar]
  38. Nehéz M., Boros P., Ferke A., Mohos J., Palotás M., Vetró G., Zimányi M., Dési I. Cytogenetic examination of people working with agrochemicals in the southern region of Hungary. Regul Toxicol Pharmacol. 1988 Mar;8(1):37–44. doi: 10.1016/0273-2300(88)90005-0. [DOI] [PubMed] [Google Scholar]
  39. Persson B., Dahlander A. M., Fredriksson M., Brage H. N., Ohlson C. G., Axelson O. Malignant lymphomas and occupational exposures. Br J Ind Med. 1989 Aug;46(8):516–520. doi: 10.1136/oem.46.8.516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Páldy A., Puskás N., Vincze K., Hadházi M. Cytogenetic studies on rural populations exposed to pesticides. Mutat Res. 1987 Mar;187(3):127–132. doi: 10.1016/0165-1218(87)90079-6. [DOI] [PubMed] [Google Scholar]
  41. Restrepo M., Muñoz N., Day N. E., Parra J. E., de Romero L., Nguyen-Dinh X. Prevalence of adverse reproductive outcomes in a population occupationally exposed to pesticides in Colombia. Scand J Work Environ Health. 1990 Aug;16(4):232–238. doi: 10.5271/sjweh.1790. [DOI] [PubMed] [Google Scholar]
  42. Rita P., Reddy P. P., Reddy S. V. Monitoring of workers occupationally exposed to pesticides in grape gardens of Andhra Pradesh. Environ Res. 1987 Oct;44(1):1–5. doi: 10.1016/s0013-9351(87)80081-6. [DOI] [PubMed] [Google Scholar]
  43. Rupa D. S., Reddy P. P., Reddi O. S. Analysis of sister-chromatid exchanges, cell kinetics and mitotic index in lymphocytes of smoking pesticide sprayers. Mutat Res. 1989 Jun;223(2):253–258. doi: 10.1016/0165-1218(89)90053-0. [DOI] [PubMed] [Google Scholar]
  44. Rupa D. S., Reddy P. P., Reddi O. S. Chromosomal aberrations in peripheral lymphocytes of cotton field workers exposed to pesticides. Environ Res. 1989 Jun;49(1):1–6. doi: 10.1016/s0013-9351(89)80017-9. [DOI] [PubMed] [Google Scholar]
  45. Rupa D. S., Reddy P. P., Reddi O. S. Frequencies of chromosomal aberrations in smokers exposed to pesticides in cotton fields. Mutat Res. 1989 Jan;222(1):37–41. doi: 10.1016/0165-1218(89)90033-5. [DOI] [PubMed] [Google Scholar]
  46. Sabbioni G., Neumann H. G. Biomonitoring of arylamines: hemoglobin adducts of urea and carbamate pesticides. Carcinogenesis. 1990 Jan;11(1):111–115. doi: 10.1093/carcin/11.1.111. [DOI] [PubMed] [Google Scholar]
  47. Saito I., Kawamura N., Uno K., Hisanaga N., Takeuchi Y., Ono Y., Iwata M., Gotoh M., Okutani H., Matsumoto T. Relationship between chlordane and its metabolites in blood of pest control operators and spraying conditions. Int Arch Occup Environ Health. 1986;58(2):91–97. doi: 10.1007/BF00380759. [DOI] [PubMed] [Google Scholar]
  48. Slomka M. B., Hine C. H. Clinical pharmacology of dichlorvos. Acta Pharmacol Toxicol (Copenh) 1981;49 (Suppl 5):105–108. doi: 10.1111/j.1600-0773.1981.tb03259.x. [DOI] [PubMed] [Google Scholar]
  49. Sorsa M. Monitoring of sister chromatid exchange and micronuclei as biological endpoints. IARC Sci Publ. 1984;(59):339–349. [PubMed] [Google Scholar]
  50. Steenland K., Carrano A., Clapp D., Ratcliffe J., Ashworth L., Meinhardt T. Cytogenetic studies in humans after short-term exposure to ethylene dibromide. J Occup Med. 1985 Oct;27(10):729–732. [PubMed] [Google Scholar]
  51. Steenland K., Carrano A., Ratcliffe J., Clapp D., Ashworth L., Meinhardt T. A cytogenetic study of papaya workers exposed to ethylene dibromide. Mutat Res. 1986 Jun;170(3):151–160. doi: 10.1016/0165-1218(86)90029-7. [DOI] [PubMed] [Google Scholar]
  52. Vainio H. Molecular approaches in toxicology: change in perspective. J Occup Environ Med. 1995 Jan;37(1):14–18. doi: 10.1097/00043764-199501000-00003. [DOI] [PubMed] [Google Scholar]
  53. Van Damme K., Casteleyn L., Heseltine E., Huici A., Sorsa M., van Larebeke N., Vineis P. Individual susceptibility and prevention of occupational diseases: scientific and ethical issues. J Occup Environ Med. 1995 Jan;37(1):91–99. doi: 10.1097/00043764-199501000-00013. [DOI] [PubMed] [Google Scholar]
  54. Walker C. H. Biochemical responses as indicators of toxic effects of chemicals in ecosystems. Toxicol Lett. 1992 Dec;64-65 Spec No:527–533. doi: 10.1016/0378-4274(92)90228-c. [DOI] [PubMed] [Google Scholar]
  55. Wiklund K., Dich J., Holm L. E., Eklund G. Risk of cancer in pesticide applicators in Swedish agriculture. Br J Ind Med. 1989 Nov;46(11):809–814. doi: 10.1136/oem.46.11.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  56. Wolfe H. R., Durham W. F., Armstrong J. F. Urinary excretion of insecticide metabolites. Excretion of para-nitrophenol and DDA as indicators of exposure to parathion. Arch Environ Health. 1970 Dec;21(6):711–716. doi: 10.1080/00039896.1970.10667324. [DOI] [PubMed] [Google Scholar]
  57. Woodruff T., Wolff M. S., Davis D. L., Hayward D. Organochlorine exposure estimation in the study of cancer etiology. Environ Res. 1994 Apr;65(1):132–144. doi: 10.1006/enrs.1994.1026. [DOI] [PubMed] [Google Scholar]
  58. Yoder J., Watson M., Benson W. W. Lymphocyte chromosome analysis of agricultural workers during extensive occupational exposure to pesticides. Mutat Res. 1973 Dec;21(6):335–340. [PubMed] [Google Scholar]
  59. Zahm S. H., Weisenburger D. D., Babbitt P. A., Saal R. C., Vaught J. B., Cantor K. P., Blair A. A case-control study of non-Hodgkin's lymphoma and the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in eastern Nebraska. Epidemiology. 1990 Sep;1(5):349–356. doi: 10.1097/00001648-199009000-00004. [DOI] [PubMed] [Google Scholar]
  60. al-Saleh I. A. Pesticides: a review article. J Environ Pathol Toxicol Oncol. 1994;13(3):151–161. [PubMed] [Google Scholar]
  61. van der Rhee H. J., Farquhar J. A., Vermeulen N. P. Efficacy and transdermal absorption of permethrin in scabies patients. Acta Derm Venereol. 1989;69(2):170–173. [PubMed] [Google Scholar]

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